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Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria.
Adv Med Sci. 2008; 53(1):80-6.AM

Abstract

PURPOSE

Chlamydia trachomatis and Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological diagnosis. To determine the occurrence of Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum in patients with sterile pyuria.

MATERIAL/METHODS

Sterile pyuria urine samples collected during the period from February 2006 to April 2007 were tested by polymerase chain reaction (PCR) for the presence of C. trachomatis, M. hominis, M. genitalium, and U. urealyticum using specific primers for each species. A total of 200 sterile pyuria samples selected from about 2400 urine samples attending the genitourinary clinic at Al-Shifa hospital, Gaza, during the period February 2006 to April 2007 and were analyzed for routine urine examination and cultured on MacConkey agar, blood agar, and sabouraud agar to detect the presence of bacteria and Candida. The 200 samples (96 male, 104 female; aged >or=18 years) containing more than 10 leukocytes / HPF and negative for culture (showing no significant growth after 24 hr) were tested by PCR for C. trachomatis and M. hominis, M. genitalium, and U. urealyticum.

RESULTS

C. trachomatis was detected in 20 samples (10%), U. urealyticum in 10 samples (5%), M. hominis in 6 samples (3%) and M. genitalium in 2 samples (1%). The difference in occurrence of C. trachomatis was statistically insignificant between males and females (P=0.509), but it was significant (P=0.008) for U. urealyticum. M. hominis was detected only in samples collected from female patients. On the other hand, M. genitalium was detected only in men.

CONCLUSION

PCR testing of sterile pyuria showed a significant number of C. trachomatis, Mycoplasma, and Ureaplasma infections. Consequently, PCR is recommended for the detection of those microorganisms in the urine samples of sterile pyuria patients.

Authors+Show Affiliations

Department of Medical Technology, Islamic University of Gaza, Palestine.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18614434

Citation

Nassar, Fadel A., et al. "Detection of Chlamydia Trachomatis and Mycoplasma Hominis, Genitalium and Ureaplasma Urealyticum By Polymerase Chain Reaction in Patients With Sterile Pyuria." Advances in Medical Sciences, vol. 53, no. 1, 2008, pp. 80-6.
Nassar FA, Abu-Elamreen FH, Shubair ME, et al. Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria. Adv Med Sci. 2008;53(1):80-6.
Nassar, F. A., Abu-Elamreen, F. H., Shubair, M. E., & Sharif, F. A. (2008). Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria. Advances in Medical Sciences, 53(1), 80-6. https://doi.org/10.2478/v10039-008-0020-1
Nassar FA, et al. Detection of Chlamydia Trachomatis and Mycoplasma Hominis, Genitalium and Ureaplasma Urealyticum By Polymerase Chain Reaction in Patients With Sterile Pyuria. Adv Med Sci. 2008;53(1):80-6. PubMed PMID: 18614434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Chlamydia trachomatis and Mycoplasma hominis, genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria. AU - Nassar,Fadel A, AU - Abu-Elamreen,Farid H, AU - Shubair,Mohammad E, AU - Sharif,Fadel A, PY - 2008/7/11/pubmed PY - 2008/11/1/medline PY - 2008/7/11/entrez SP - 80 EP - 6 JF - Advances in medical sciences JO - Adv Med Sci VL - 53 IS - 1 N2 - PURPOSE: Chlamydia trachomatis and Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum are associated with various diseases of the urogenital tract, but they are usually not detected by routine microbiological diagnosis. To determine the occurrence of Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, and Ureaplasma urealyticum in patients with sterile pyuria. MATERIAL/METHODS: Sterile pyuria urine samples collected during the period from February 2006 to April 2007 were tested by polymerase chain reaction (PCR) for the presence of C. trachomatis, M. hominis, M. genitalium, and U. urealyticum using specific primers for each species. A total of 200 sterile pyuria samples selected from about 2400 urine samples attending the genitourinary clinic at Al-Shifa hospital, Gaza, during the period February 2006 to April 2007 and were analyzed for routine urine examination and cultured on MacConkey agar, blood agar, and sabouraud agar to detect the presence of bacteria and Candida. The 200 samples (96 male, 104 female; aged >or=18 years) containing more than 10 leukocytes / HPF and negative for culture (showing no significant growth after 24 hr) were tested by PCR for C. trachomatis and M. hominis, M. genitalium, and U. urealyticum. RESULTS: C. trachomatis was detected in 20 samples (10%), U. urealyticum in 10 samples (5%), M. hominis in 6 samples (3%) and M. genitalium in 2 samples (1%). The difference in occurrence of C. trachomatis was statistically insignificant between males and females (P=0.509), but it was significant (P=0.008) for U. urealyticum. M. hominis was detected only in samples collected from female patients. On the other hand, M. genitalium was detected only in men. CONCLUSION: PCR testing of sterile pyuria showed a significant number of C. trachomatis, Mycoplasma, and Ureaplasma infections. Consequently, PCR is recommended for the detection of those microorganisms in the urine samples of sterile pyuria patients. SN - 1896-1126 UR - https://www.unboundmedicine.com/medline/citation/18614434/Detection_of_Chlamydia_trachomatis_and_Mycoplasma_hominis_genitalium_and_Ureaplasma_urealyticum_by_polymerase_chain_reaction_in_patients_with_sterile_pyuria_ L2 - http://www.diseaseinfosearch.org/result/9709 DB - PRIME DP - Unbound Medicine ER -