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Comparison of two extraction procedures for determination of drugs of abuse in human saliva by high-performance liquid chromatography.
J Appl Toxicol. 2008 Nov; 28(8):998-1003.JA

Abstract

High performance liquid chromatography in combination with diode array detection (HPLC-DAD) was used to determine morphine, 6-acetylmorphine, cocaine, benzoylecgonine, cocaethylene, methadone and 2-ethylene-1,5-dimethyl-3,3,-diphenylpyrrolidine in human saliva. For comparison, samples were prepared by either liquid-liquid extraction in Toxitubes A or microwave-assisted extraction (MAE), by mixing 1 ml of saliva with 10 ml of chloroform and operating at 100 degrees C for 10 min. Acetonitrile and 0.02 m phosphate buffer at pH 6.5 were used as mobile phase in HPLC in gradient mode. The detector response was linear over the drug concentration range of 0.05-2.0 microg ml(-1) in human saliva. The analytical method was validated by determining its precision and accuracy (n = 5), which were lower than 5% as relative standard deviation and 6% as relative error. Limits of detection ranged from 10 to 35 ng ml(-1); mean recoveries of drugs were from 53 to 95% with Toxitubes A and from 83 to 100% with MAE at two different concentrations (0.1 and 1.0 microg ml(-1)). The proposed method was applied to 24 saliva samples from individuals poisoned with opiates and/or cocaine.

Authors+Show Affiliations

Institute of Legal Medicine, Forensic Toxicology Service, Faculty of Medicine, Santiago de Compostela, Spain. imlpuri@usc.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18615842

Citation

Fernández, P, et al. "Comparison of Two Extraction Procedures for Determination of Drugs of Abuse in Human Saliva By High-performance Liquid Chromatography." Journal of Applied Toxicology : JAT, vol. 28, no. 8, 2008, pp. 998-1003.
Fernández P, Morales L, Vázquez C, et al. Comparison of two extraction procedures for determination of drugs of abuse in human saliva by high-performance liquid chromatography. J Appl Toxicol. 2008;28(8):998-1003.
Fernández, P., Morales, L., Vázquez, C., Lago, M., & Bermejo, A. M. (2008). Comparison of two extraction procedures for determination of drugs of abuse in human saliva by high-performance liquid chromatography. Journal of Applied Toxicology : JAT, 28(8), 998-1003. https://doi.org/10.1002/jat.1365
Fernández P, et al. Comparison of Two Extraction Procedures for Determination of Drugs of Abuse in Human Saliva By High-performance Liquid Chromatography. J Appl Toxicol. 2008;28(8):998-1003. PubMed PMID: 18615842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of two extraction procedures for determination of drugs of abuse in human saliva by high-performance liquid chromatography. AU - Fernández,P, AU - Morales,L, AU - Vázquez,C, AU - Lago,M, AU - Bermejo,A M, PY - 2008/7/11/pubmed PY - 2009/1/6/medline PY - 2008/7/11/entrez SP - 998 EP - 1003 JF - Journal of applied toxicology : JAT JO - J Appl Toxicol VL - 28 IS - 8 N2 - High performance liquid chromatography in combination with diode array detection (HPLC-DAD) was used to determine morphine, 6-acetylmorphine, cocaine, benzoylecgonine, cocaethylene, methadone and 2-ethylene-1,5-dimethyl-3,3,-diphenylpyrrolidine in human saliva. For comparison, samples were prepared by either liquid-liquid extraction in Toxitubes A or microwave-assisted extraction (MAE), by mixing 1 ml of saliva with 10 ml of chloroform and operating at 100 degrees C for 10 min. Acetonitrile and 0.02 m phosphate buffer at pH 6.5 were used as mobile phase in HPLC in gradient mode. The detector response was linear over the drug concentration range of 0.05-2.0 microg ml(-1) in human saliva. The analytical method was validated by determining its precision and accuracy (n = 5), which were lower than 5% as relative standard deviation and 6% as relative error. Limits of detection ranged from 10 to 35 ng ml(-1); mean recoveries of drugs were from 53 to 95% with Toxitubes A and from 83 to 100% with MAE at two different concentrations (0.1 and 1.0 microg ml(-1)). The proposed method was applied to 24 saliva samples from individuals poisoned with opiates and/or cocaine. SN - 0260-437X UR - https://www.unboundmedicine.com/medline/citation/18615842/Comparison_of_two_extraction_procedures_for_determination_of_drugs_of_abuse_in_human_saliva_by_high_performance_liquid_chromatography_ L2 - https://doi.org/10.1002/jat.1365 DB - PRIME DP - Unbound Medicine ER -