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Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole-3-acetic acid in plants.
Phytochem Anal. 2008 Nov-Dec; 19(6):560-7.PA

Abstract

INTRODUCTION

Jasmonic acid (JA), abscisic acid (ABA) and indole-3-acetic acid (IAA) are important plant hormones. Plant hormones are difficult to analyse because they occur in small concentrations and other substances in the plant interfere with their detection.

OBJECTIVE

To develop a new, inexpensive procedure for the rapid extraction and purification of IAA, ABA and JA from various plant species.

METHODOLOGY

Samples were prepared by extraction of plant tissues with methanol and ethyl acetate. Then the extracts were further purified and enriched with C(18) cartridges. The final extracts were derivatised with diazomethane and then measured by GC-MS. The results of the new methodology were compared with those of the Creelman and Mullet procedure.

RESULTS

Sequential elution of the assimilates from the C(18)cartridges revealed that IAA and ABA eluted in 40% methanol, while JA subsequently eluted in 60% methanol. The new plant hormone extraction and purification procedure produced results that were comparable to those obtained with the Creelman and Mullet's procedure. This new procedure requires only 0.5 g leaf samples to quantify these compounds with high reliability and can simultaneously determine the concentrations of the three plant hormones.

CONCLUSION

A simple, inexpensive method was developed for determining endogenous IAA, ABA and JA concentrations in plant tissue.

Authors+Show Affiliations

Department of Life Science, Hebei Normal University of Science and Technology, Changli, Hebei 066600, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18618474

Citation

Zhang, Feng Juan, et al. "Study On the Extraction, Purification and Quantification of Jasmonic Acid, Abscisic Acid and Indole-3-acetic Acid in Plants." Phytochemical Analysis : PCA, vol. 19, no. 6, 2008, pp. 560-7.
Zhang FJ, Jin YJ, Xu XY, et al. Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole-3-acetic acid in plants. Phytochem Anal. 2008;19(6):560-7.
Zhang, F. J., Jin, Y. J., Xu, X. Y., Lu, R. C., & Chen, H. J. (2008). Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole-3-acetic acid in plants. Phytochemical Analysis : PCA, 19(6), 560-7. https://doi.org/10.1002/pca.1085
Zhang FJ, et al. Study On the Extraction, Purification and Quantification of Jasmonic Acid, Abscisic Acid and Indole-3-acetic Acid in Plants. Phytochem Anal. 2008 Nov-Dec;19(6):560-7. PubMed PMID: 18618474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole-3-acetic acid in plants. AU - Zhang,Feng Juan, AU - Jin,You Ju, AU - Xu,Xing You, AU - Lu,Rong Chun, AU - Chen,Hua Jun, PY - 2008/7/12/pubmed PY - 2009/1/10/medline PY - 2008/7/12/entrez SP - 560 EP - 7 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 19 IS - 6 N2 - INTRODUCTION: Jasmonic acid (JA), abscisic acid (ABA) and indole-3-acetic acid (IAA) are important plant hormones. Plant hormones are difficult to analyse because they occur in small concentrations and other substances in the plant interfere with their detection. OBJECTIVE: To develop a new, inexpensive procedure for the rapid extraction and purification of IAA, ABA and JA from various plant species. METHODOLOGY: Samples were prepared by extraction of plant tissues with methanol and ethyl acetate. Then the extracts were further purified and enriched with C(18) cartridges. The final extracts were derivatised with diazomethane and then measured by GC-MS. The results of the new methodology were compared with those of the Creelman and Mullet procedure. RESULTS: Sequential elution of the assimilates from the C(18)cartridges revealed that IAA and ABA eluted in 40% methanol, while JA subsequently eluted in 60% methanol. The new plant hormone extraction and purification procedure produced results that were comparable to those obtained with the Creelman and Mullet's procedure. This new procedure requires only 0.5 g leaf samples to quantify these compounds with high reliability and can simultaneously determine the concentrations of the three plant hormones. CONCLUSION: A simple, inexpensive method was developed for determining endogenous IAA, ABA and JA concentrations in plant tissue. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/18618474/Study_on_the_extraction_purification_and_quantification_of_jasmonic_acid_abscisic_acid_and_indole_3_acetic_acid_in_plants_ L2 - https://doi.org/10.1002/pca.1085 DB - PRIME DP - Unbound Medicine ER -