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Characterization of midgut trypsinogen-like cDNA and enzymatic activity in Plutella xylostella parasitized by Cotesia vestalis or Diadegma semiclausum.
Arch Insect Biochem Physiol. 2009 Jan; 70(1):3-17.AI

Abstract

Protein digestion in insects is a result of the action of a complex of proteinases present in the midgut. In this report we describe the cloning and sequencing of a trypsin cDNA from larvae of the lepidopteran herbivore Plutella xylostella. We investigated the expression of this gene and enzymatic activity of its translation product with N-a-benzoyl-l-arginine p-nitroanilide (BApNA) as substrate in P. xylostella larvae that were either unparasitized or parasitized by Cotesia vestalis or Diadegma semiclausum parasitoids. The full cDNA sequence consisted of an open reading frame (ORF) encoding 273 amino acid residues including 23 residues of a signal peptide, and the predicted mature trypsinogen-like enzyme had a molecular mass of 26.5 kDa. The amino acid sequence of this trypsinogen-like enzyme protein and phylogenetic relationship with other published trypsin enzyme proteins suggested that it may be a new proteinase in the trypsin protein family. Parasitism of D. semiclausum did not significantly change the mRNA transcript level or BApNAase activity in host larvae. By contrast, parasitization by C. vestalis induced higher transcript levels coupled with a higher level of BApNAase activity. The BApNAase activity in the midgut of nonparasitized or parasitized P. xylostella larvae increased to a maximum level at pH 12, and the parasitism by both C. vestalis and D. semiclausum increased sensitivity of the enzyme to pH values ranging from 2 to 9.5. These parasitoid-induced changes may represent host manipulation by the developing parasitoid larva.

Authors+Show Affiliations

Institute of Insect Sciences, Zhejiang University, Hangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18618770

Citation

Shi, Min, et al. "Characterization of Midgut Trypsinogen-like cDNA and Enzymatic Activity in Plutella Xylostella Parasitized By Cotesia Vestalis or Diadegma Semiclausum." Archives of Insect Biochemistry and Physiology, vol. 70, no. 1, 2009, pp. 3-17.
Shi M, Huang F, Chen YF, et al. Characterization of midgut trypsinogen-like cDNA and enzymatic activity in Plutella xylostella parasitized by Cotesia vestalis or Diadegma semiclausum. Arch Insect Biochem Physiol. 2009;70(1):3-17.
Shi, M., Huang, F., Chen, Y. F., Meng, X. F., & Chen, X. X. (2009). Characterization of midgut trypsinogen-like cDNA and enzymatic activity in Plutella xylostella parasitized by Cotesia vestalis or Diadegma semiclausum. Archives of Insect Biochemistry and Physiology, 70(1), 3-17. https://doi.org/10.1002/arch.20249
Shi M, et al. Characterization of Midgut Trypsinogen-like cDNA and Enzymatic Activity in Plutella Xylostella Parasitized By Cotesia Vestalis or Diadegma Semiclausum. Arch Insect Biochem Physiol. 2009;70(1):3-17. PubMed PMID: 18618770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of midgut trypsinogen-like cDNA and enzymatic activity in Plutella xylostella parasitized by Cotesia vestalis or Diadegma semiclausum. AU - Shi,Min, AU - Huang,Fang, AU - Chen,Ya-Feng, AU - Meng,Xiang-Feng, AU - Chen,Xue-Xin, PY - 2008/7/12/entrez PY - 2008/7/12/pubmed PY - 2009/2/6/medline SP - 3 EP - 17 JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 70 IS - 1 N2 - Protein digestion in insects is a result of the action of a complex of proteinases present in the midgut. In this report we describe the cloning and sequencing of a trypsin cDNA from larvae of the lepidopteran herbivore Plutella xylostella. We investigated the expression of this gene and enzymatic activity of its translation product with N-a-benzoyl-l-arginine p-nitroanilide (BApNA) as substrate in P. xylostella larvae that were either unparasitized or parasitized by Cotesia vestalis or Diadegma semiclausum parasitoids. The full cDNA sequence consisted of an open reading frame (ORF) encoding 273 amino acid residues including 23 residues of a signal peptide, and the predicted mature trypsinogen-like enzyme had a molecular mass of 26.5 kDa. The amino acid sequence of this trypsinogen-like enzyme protein and phylogenetic relationship with other published trypsin enzyme proteins suggested that it may be a new proteinase in the trypsin protein family. Parasitism of D. semiclausum did not significantly change the mRNA transcript level or BApNAase activity in host larvae. By contrast, parasitization by C. vestalis induced higher transcript levels coupled with a higher level of BApNAase activity. The BApNAase activity in the midgut of nonparasitized or parasitized P. xylostella larvae increased to a maximum level at pH 12, and the parasitism by both C. vestalis and D. semiclausum increased sensitivity of the enzyme to pH values ranging from 2 to 9.5. These parasitoid-induced changes may represent host manipulation by the developing parasitoid larva. SN - 1520-6327 UR - https://www.unboundmedicine.com/medline/citation/18618770/Characterization_of_midgut_trypsinogen_like_cDNA_and_enzymatic_activity_in_Plutella_xylostella_parasitized_by_Cotesia_vestalis_or_Diadegma_semiclausum_ L2 - https://doi.org/10.1002/arch.20249 DB - PRIME DP - Unbound Medicine ER -