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Down-regulation of outer membrane proteins by noncoding RNAs: unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX regulatory case.
J Mol Biol. 2008 Oct 31; 383(1):1-9.JM

Abstract

The sigma(E) (extracytoplasmic stress response sigma factor in Escherichia coli) signaling system of Gram-negative bacteria plays an essential role in the maintenance of the extracytoplasmic compartment. Upon induction of this system, approximately 100 genes are up-regulated. The majority of these genes encode proteins that participate in the synthesis, assembly, and homeostasis of outer membrane proteins and lipopolysaccharides. A second aspect of the sigma(E) response is a regulatory loop that prevents expression of the major porins. Misfolding or overproduction of most of these porins is sufficient to trigger the envelope stress response. Recent work indicates that small Hfq-binding RNAs play a major role in maintaining envelope homeostasis and, so far, two sigma(E)-dependent small noncoding RNAs (sRNAs), MicA and RybB, have been shown to facilitate rapid removal of multiple omp transcripts in response to elevated activity of the alternative sigma factor. Here we report the identification of the sRNA (CyaR, cyclic AMP-activated RNA) that promotes decay of the ompX mRNA. The transcription of the cyaR gene is stringently controlled by cAMP-cAMP receptor protein and, unexpectedly, cyaR expression is also up-regulated, directly or indirectly, by sigma(E). In addition, this work identified MicA as a factor that cooperates in the negative control of ompX expression. The conservation of CyaR, MicA, RybB, and their targets suggests that the omp mRNA-sRNA regulatory network is an integral part of the envelope stress response in many enterobacteria.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18619465

Citation

Johansen, Jesper, et al. "Down-regulation of Outer Membrane Proteins By Noncoding RNAs: Unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX Regulatory Case." Journal of Molecular Biology, vol. 383, no. 1, 2008, pp. 1-9.
Johansen J, Eriksen M, Kallipolitis B, et al. Down-regulation of outer membrane proteins by noncoding RNAs: unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX regulatory case. J Mol Biol. 2008;383(1):1-9.
Johansen, J., Eriksen, M., Kallipolitis, B., & Valentin-Hansen, P. (2008). Down-regulation of outer membrane proteins by noncoding RNAs: unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX regulatory case. Journal of Molecular Biology, 383(1), 1-9. https://doi.org/10.1016/j.jmb.2008.06.058
Johansen J, et al. Down-regulation of Outer Membrane Proteins By Noncoding RNAs: Unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX Regulatory Case. J Mol Biol. 2008 Oct 31;383(1):1-9. PubMed PMID: 18619465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Down-regulation of outer membrane proteins by noncoding RNAs: unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX regulatory case. AU - Johansen,Jesper, AU - Eriksen,Maiken, AU - Kallipolitis,Birgitte, AU - Valentin-Hansen,Poul, Y1 - 2008/06/28/ PY - 2008/03/16/received PY - 2008/06/18/revised PY - 2008/06/23/accepted PY - 2008/7/16/pubmed PY - 2008/11/13/medline PY - 2008/7/16/entrez SP - 1 EP - 9 JF - Journal of molecular biology JO - J Mol Biol VL - 383 IS - 1 N2 - The sigma(E) (extracytoplasmic stress response sigma factor in Escherichia coli) signaling system of Gram-negative bacteria plays an essential role in the maintenance of the extracytoplasmic compartment. Upon induction of this system, approximately 100 genes are up-regulated. The majority of these genes encode proteins that participate in the synthesis, assembly, and homeostasis of outer membrane proteins and lipopolysaccharides. A second aspect of the sigma(E) response is a regulatory loop that prevents expression of the major porins. Misfolding or overproduction of most of these porins is sufficient to trigger the envelope stress response. Recent work indicates that small Hfq-binding RNAs play a major role in maintaining envelope homeostasis and, so far, two sigma(E)-dependent small noncoding RNAs (sRNAs), MicA and RybB, have been shown to facilitate rapid removal of multiple omp transcripts in response to elevated activity of the alternative sigma factor. Here we report the identification of the sRNA (CyaR, cyclic AMP-activated RNA) that promotes decay of the ompX mRNA. The transcription of the cyaR gene is stringently controlled by cAMP-cAMP receptor protein and, unexpectedly, cyaR expression is also up-regulated, directly or indirectly, by sigma(E). In addition, this work identified MicA as a factor that cooperates in the negative control of ompX expression. The conservation of CyaR, MicA, RybB, and their targets suggests that the omp mRNA-sRNA regulatory network is an integral part of the envelope stress response in many enterobacteria. SN - 1089-8638 UR - https://www.unboundmedicine.com/medline/citation/18619465/Down_regulation_of_outer_membrane_proteins_by_noncoding_RNAs:_unraveling_the_cAMP_CRP__and_sigmaE_dependent_CyaR_ompX_regulatory_case_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(08)00774-2 DB - PRIME DP - Unbound Medicine ER -