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Wound closure and metabolic parameter variability in a db/db mouse model for diabetic ulcers.
J Surg Res 2009; 151(1):100-7JS

Abstract

BACKGROUND

Diabetic foot ulcers are a major cause of nontraumatic lower extremity amputations. Wound-healing researchers commonly use db/db mice as a model for diabetes, while the excisional wound correlates well with chronic foot ulcers. Recent clinical trials identified a correlation between glycemic control and cardiovascular complications in diabetic patients. The purpose of this study was to determine if the severity of diabetes was related to poor wound healing and the broad wound closure variability observed in diabetic db/db mice.

MATERIALS AND METHODS

Adult female C57BLKS/J, db+/-, and db/db mice were anesthetized followed by creation of a 1.5 x 1.5 cm full-thickness excisional wound. Wound closure was measured on postoperative days (PODs) 1, 5, 7, 10, 14, and 21. Weight, fasting blood glucose, and fasting insulin were also measured during the study.

RESULTS

By POD 21 both wild-type and db+/- mice demonstrated complete wound closure. In db/db mice open wounds were still present at POD 21. There was a broad range of percent wound closure from 24 to 81% with a mean of 55%. Despite strong correlations between diabetic parameters, there was no significant correlation between wound closure rate and severity of diabetes.

CONCLUSIONS

Diabetic db/db mice exhibit a significant impairment of healing in the excisional wound model. The variability of wound closure for individual mice did not correlate with severity of obesity, hyperglycemia, hyperinsulinemia, or insulin resistance. An extensive evaluation of basic diabetes parameters does not provide significant insight into the wound-healing process in the db/db mouse model.

Authors+Show Affiliations

Department of OB/GYN, Columbia University Medical Center, New York, New York, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18619614

Citation

Trousdale, Rhonda K., et al. "Wound Closure and Metabolic Parameter Variability in a Db/db Mouse Model for Diabetic Ulcers." The Journal of Surgical Research, vol. 151, no. 1, 2009, pp. 100-7.
Trousdale RK, Jacobs S, Simhaee DA, et al. Wound closure and metabolic parameter variability in a db/db mouse model for diabetic ulcers. J Surg Res. 2009;151(1):100-7.
Trousdale, R. K., Jacobs, S., Simhaee, D. A., Wu, J. K., & Lustbader, J. W. (2009). Wound closure and metabolic parameter variability in a db/db mouse model for diabetic ulcers. The Journal of Surgical Research, 151(1), pp. 100-7. doi:10.1016/j.jss.2008.01.023.
Trousdale RK, et al. Wound Closure and Metabolic Parameter Variability in a Db/db Mouse Model for Diabetic Ulcers. J Surg Res. 2009;151(1):100-7. PubMed PMID: 18619614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Wound closure and metabolic parameter variability in a db/db mouse model for diabetic ulcers. AU - Trousdale,Rhonda K, AU - Jacobs,Sharone', AU - Simhaee,David A, AU - Wu,June K, AU - Lustbader,Joyce W, Y1 - 2008/03/03/ PY - 2007/10/12/received PY - 2007/12/10/revised PY - 2008/01/16/accepted PY - 2008/7/16/entrez PY - 2008/7/16/pubmed PY - 2009/1/15/medline SP - 100 EP - 7 JF - The Journal of surgical research JO - J. Surg. Res. VL - 151 IS - 1 N2 - BACKGROUND: Diabetic foot ulcers are a major cause of nontraumatic lower extremity amputations. Wound-healing researchers commonly use db/db mice as a model for diabetes, while the excisional wound correlates well with chronic foot ulcers. Recent clinical trials identified a correlation between glycemic control and cardiovascular complications in diabetic patients. The purpose of this study was to determine if the severity of diabetes was related to poor wound healing and the broad wound closure variability observed in diabetic db/db mice. MATERIALS AND METHODS: Adult female C57BLKS/J, db+/-, and db/db mice were anesthetized followed by creation of a 1.5 x 1.5 cm full-thickness excisional wound. Wound closure was measured on postoperative days (PODs) 1, 5, 7, 10, 14, and 21. Weight, fasting blood glucose, and fasting insulin were also measured during the study. RESULTS: By POD 21 both wild-type and db+/- mice demonstrated complete wound closure. In db/db mice open wounds were still present at POD 21. There was a broad range of percent wound closure from 24 to 81% with a mean of 55%. Despite strong correlations between diabetic parameters, there was no significant correlation between wound closure rate and severity of diabetes. CONCLUSIONS: Diabetic db/db mice exhibit a significant impairment of healing in the excisional wound model. The variability of wound closure for individual mice did not correlate with severity of obesity, hyperglycemia, hyperinsulinemia, or insulin resistance. An extensive evaluation of basic diabetes parameters does not provide significant insight into the wound-healing process in the db/db mouse model. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/18619614/Wound_closure_and_metabolic_parameter_variability_in_a_db/db_mouse_model_for_diabetic_ulcers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(08)00047-4 DB - PRIME DP - Unbound Medicine ER -