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The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome.
BMC Genomics. 2008 Jul 11; 9:327.BG

Abstract

BACKGROUND

The hypercellulolytic mutant Hypocrea jecorina (anamorph Trichoderma reesei) RUT C30 is the H. jecorina strain most frequently used for cellulase fermentations and has also often been employed for basic research on cellulase regulation. This strain has been reported to contain a truncated carbon catabolite repressor gene cre1 and is consequently carbon catabolite derepressed. To date this and an additional frame-shift mutation in the glycoprotein-processing beta-glucosidase II encoding gene are the only known genetic differences in strain RUT C30.

RESULTS

In the present paper we show that H. jecorina RUT C30 lacks an 85 kb genomic fragment, and consequently misses additional 29 genes comprising transcription factors, enzymes of the primary metabolism and transport proteins. This loss is already present in the ancestor of RUT C30--NG 14--and seems to have occurred in a palindromic AT-rich repeat (PATRR) typically inducing chromosomal translocations, and is not linked to the cre1 locus. The mutation of the cre1 locus has specifically occurred in RUT C30. Some of the genes that are lacking in RUT C30 could be correlated with pronounced alterations in its phenotype, such as poor growth on alpha-linked oligo- and polyglucosides (loss of maltose permease), or disturbance of osmotic homeostasis.

CONCLUSION

Our data place a general caveat on the use of H. jecorina RUT C30 for further basic research.

Authors+Show Affiliations

Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/166-5, A-1060 Wien, Austria. vseidl@mail.zserv.tuwien.ac.atNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18620557

Citation

Seidl, Verena, et al. "The Hypocrea Jecorina (Trichoderma Reesei) Hypercellulolytic Mutant RUT C30 Lacks a 85 Kb (29 Gene-encoding) Region of the Wild-type Genome." BMC Genomics, vol. 9, 2008, p. 327.
Seidl V, Gamauf C, Druzhinina IS, et al. The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome. BMC Genomics. 2008;9:327.
Seidl, V., Gamauf, C., Druzhinina, I. S., Seiboth, B., Hartl, L., & Kubicek, C. P. (2008). The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome. BMC Genomics, 9, 327. https://doi.org/10.1186/1471-2164-9-327
Seidl V, et al. The Hypocrea Jecorina (Trichoderma Reesei) Hypercellulolytic Mutant RUT C30 Lacks a 85 Kb (29 Gene-encoding) Region of the Wild-type Genome. BMC Genomics. 2008 Jul 11;9:327. PubMed PMID: 18620557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Hypocrea jecorina (Trichoderma reesei) hypercellulolytic mutant RUT C30 lacks a 85 kb (29 gene-encoding) region of the wild-type genome. AU - Seidl,Verena, AU - Gamauf,Christian, AU - Druzhinina,Irina S, AU - Seiboth,Bernhard, AU - Hartl,Lukas, AU - Kubicek,Christian P, Y1 - 2008/07/11/ PY - 2007/10/28/received PY - 2008/07/11/accepted PY - 2008/7/16/pubmed PY - 2008/8/20/medline PY - 2008/7/16/entrez SP - 327 EP - 327 JF - BMC genomics JO - BMC Genomics VL - 9 N2 - BACKGROUND: The hypercellulolytic mutant Hypocrea jecorina (anamorph Trichoderma reesei) RUT C30 is the H. jecorina strain most frequently used for cellulase fermentations and has also often been employed for basic research on cellulase regulation. This strain has been reported to contain a truncated carbon catabolite repressor gene cre1 and is consequently carbon catabolite derepressed. To date this and an additional frame-shift mutation in the glycoprotein-processing beta-glucosidase II encoding gene are the only known genetic differences in strain RUT C30. RESULTS: In the present paper we show that H. jecorina RUT C30 lacks an 85 kb genomic fragment, and consequently misses additional 29 genes comprising transcription factors, enzymes of the primary metabolism and transport proteins. This loss is already present in the ancestor of RUT C30--NG 14--and seems to have occurred in a palindromic AT-rich repeat (PATRR) typically inducing chromosomal translocations, and is not linked to the cre1 locus. The mutation of the cre1 locus has specifically occurred in RUT C30. Some of the genes that are lacking in RUT C30 could be correlated with pronounced alterations in its phenotype, such as poor growth on alpha-linked oligo- and polyglucosides (loss of maltose permease), or disturbance of osmotic homeostasis. CONCLUSION: Our data place a general caveat on the use of H. jecorina RUT C30 for further basic research. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/18620557/The_Hypocrea_jecorina__Trichoderma_reesei__hypercellulolytic_mutant_RUT_C30_lacks_a_85_kb__29_gene_encoding__region_of_the_wild_type_genome_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-9-327 DB - PRIME DP - Unbound Medicine ER -