Tags

Type your tag names separated by a space and hit enter

Detection of ciguatoxin in fish tissue using sandwich ELISA and neuroblastoma cell bioassay.
J Clin Lab Anal. 2008; 22(4):246-53.JC

Abstract

The applicability of a new enzyme-linked immunoassay (ELISA) for detecting ciguatoxin (CTX) in fish tissue was evaluated by testing three fish species commonly implicated in ciguatera fish poisoning in Hawaii. A total of 164 individual almaco jack (Seriola rivoliana) and greater amberjack (S. dumerili) and a total of 175 individuals of the blue-spotted grouper (Cephalopholis argus) were caught at various locations in the Hawaiian Islands. Muscle tissue from each individual was assessed for the presence of CTX using two methods: a semi-quantitative ELISA that was recently developed for detecting picogram levels of CTX in fish extract and a neuroblastoma (NB) cell assay commonly used to screen for marine toxins in fish. Results of the tests were highly correlated, with the ELISA indicating the presence of CTX in 9.4% of all fish samples, and the NB assay indicating toxicity in 6.8% of the fish samples. We conclude that the ELISA produces reliable and accurate results that are consistent with those provided by the accepted NB assay and that the ELISA has potential for future applications in screening fish populations for CTX.

Authors+Show Affiliations

Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA. empey@hawaii.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18623118

Citation

Empey Campora, Cara, et al. "Detection of Ciguatoxin in Fish Tissue Using Sandwich ELISA and Neuroblastoma Cell Bioassay." Journal of Clinical Laboratory Analysis, vol. 22, no. 4, 2008, pp. 246-53.
Empey Campora C, Dierking J, Tamaru CS, et al. Detection of ciguatoxin in fish tissue using sandwich ELISA and neuroblastoma cell bioassay. J Clin Lab Anal. 2008;22(4):246-53.
Empey Campora, C., Dierking, J., Tamaru, C. S., Hokama, Y., & Vincent, D. (2008). Detection of ciguatoxin in fish tissue using sandwich ELISA and neuroblastoma cell bioassay. Journal of Clinical Laboratory Analysis, 22(4), 246-53. https://doi.org/10.1002/jcla.20250
Empey Campora C, et al. Detection of Ciguatoxin in Fish Tissue Using Sandwich ELISA and Neuroblastoma Cell Bioassay. J Clin Lab Anal. 2008;22(4):246-53. PubMed PMID: 18623118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of ciguatoxin in fish tissue using sandwich ELISA and neuroblastoma cell bioassay. AU - Empey Campora,Cara, AU - Dierking,Jan, AU - Tamaru,Clyde S, AU - Hokama,Yoshitsugi, AU - Vincent,Douglas, PY - 2008/7/16/pubmed PY - 2008/9/5/medline PY - 2008/7/16/entrez SP - 246 EP - 53 JF - Journal of clinical laboratory analysis JO - J Clin Lab Anal VL - 22 IS - 4 N2 - The applicability of a new enzyme-linked immunoassay (ELISA) for detecting ciguatoxin (CTX) in fish tissue was evaluated by testing three fish species commonly implicated in ciguatera fish poisoning in Hawaii. A total of 164 individual almaco jack (Seriola rivoliana) and greater amberjack (S. dumerili) and a total of 175 individuals of the blue-spotted grouper (Cephalopholis argus) were caught at various locations in the Hawaiian Islands. Muscle tissue from each individual was assessed for the presence of CTX using two methods: a semi-quantitative ELISA that was recently developed for detecting picogram levels of CTX in fish extract and a neuroblastoma (NB) cell assay commonly used to screen for marine toxins in fish. Results of the tests were highly correlated, with the ELISA indicating the presence of CTX in 9.4% of all fish samples, and the NB assay indicating toxicity in 6.8% of the fish samples. We conclude that the ELISA produces reliable and accurate results that are consistent with those provided by the accepted NB assay and that the ELISA has potential for future applications in screening fish populations for CTX. SN - 1098-2825 UR - https://www.unboundmedicine.com/medline/citation/18623118/Detection_of_ciguatoxin_in_fish_tissue_using_sandwich_ELISA_and_neuroblastoma_cell_bioassay_ L2 - https://doi.org/10.1002/jcla.20250 DB - PRIME DP - Unbound Medicine ER -