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The response of the yeast Saccharomyces cerevisiae to sudden vs. gradual changes in environmental stress monitored by expression of the stress response protein Hsp12p.
FEMS Yeast Res. 2008 Sep; 8(6):829-38.FY

Abstract

The response of the yeast Saccharomyces cerevisiae to sudden vs. gradual changes in different environmental stress conditions during both respiratory growth and aerobic fermentative growth in the presence of excess glucose was investigated by monitoring the level and rate of expression of the stress response protein Hsp12p using the fluorescent fusion construct Hsp12p-Gfp2p. The initial expression level and the rate of Hsp12p synthesis was significantly greater under glucose-limited conditions in the chemostat (D<0.14 h(-1)) compared with when excess glucose was present in the auxostat. Decreasing the dilution rate and the glucose concentration further in the A-stat resulted in increased Hsp12p expression, which was more marked when a rapid rather than a gradual change was affected. Common stress factors such as NaCl, ethanol and elevated temperature caused stress responses in both D-stat and auxo-accelerostat culture. The magnitude of the stress response depended on the stress factor, cultivation conditions as well as the rate of change of the stress factor. The rate of Hsp12p synthesis increased due to all applied stresses, with the observed increase between 2 and 20 times lower when the stress was applied gradually rather than rapidly. The results suggested that the Hsp12p expression rate is a good indicator of applied stress in S. cerevisiae.

Authors+Show Affiliations

Competence Centre of Food and Fermentation Technologies, Akadeemia tee, Tallinn, Estonia. ildar.nisamedtinov@ttu.eeNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

18625028

Citation

Nisamedtinov, Ildar, et al. "The Response of the Yeast Saccharomyces Cerevisiae to Sudden Vs. Gradual Changes in Environmental Stress Monitored By Expression of the Stress Response Protein Hsp12p." FEMS Yeast Research, vol. 8, no. 6, 2008, pp. 829-38.
Nisamedtinov I, Lindsey GG, Karreman R, et al. The response of the yeast Saccharomyces cerevisiae to sudden vs. gradual changes in environmental stress monitored by expression of the stress response protein Hsp12p. FEMS Yeast Res. 2008;8(6):829-38.
Nisamedtinov, I., Lindsey, G. G., Karreman, R., Orumets, K., Koplimaa, M., Kevvai, K., & Paalme, T. (2008). The response of the yeast Saccharomyces cerevisiae to sudden vs. gradual changes in environmental stress monitored by expression of the stress response protein Hsp12p. FEMS Yeast Research, 8(6), 829-38. https://doi.org/10.1111/j.1567-1364.2008.00391.x
Nisamedtinov I, et al. The Response of the Yeast Saccharomyces Cerevisiae to Sudden Vs. Gradual Changes in Environmental Stress Monitored By Expression of the Stress Response Protein Hsp12p. FEMS Yeast Res. 2008;8(6):829-38. PubMed PMID: 18625028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The response of the yeast Saccharomyces cerevisiae to sudden vs. gradual changes in environmental stress monitored by expression of the stress response protein Hsp12p. AU - Nisamedtinov,Ildar, AU - Lindsey,George G, AU - Karreman,Robert, AU - Orumets,Kerti, AU - Koplimaa,Mariane, AU - Kevvai,Kaspar, AU - Paalme,Toomas, Y1 - 2008/07/08/ PY - 2008/7/16/pubmed PY - 2008/12/17/medline PY - 2008/7/16/entrez SP - 829 EP - 38 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 6 N2 - The response of the yeast Saccharomyces cerevisiae to sudden vs. gradual changes in different environmental stress conditions during both respiratory growth and aerobic fermentative growth in the presence of excess glucose was investigated by monitoring the level and rate of expression of the stress response protein Hsp12p using the fluorescent fusion construct Hsp12p-Gfp2p. The initial expression level and the rate of Hsp12p synthesis was significantly greater under glucose-limited conditions in the chemostat (D<0.14 h(-1)) compared with when excess glucose was present in the auxostat. Decreasing the dilution rate and the glucose concentration further in the A-stat resulted in increased Hsp12p expression, which was more marked when a rapid rather than a gradual change was affected. Common stress factors such as NaCl, ethanol and elevated temperature caused stress responses in both D-stat and auxo-accelerostat culture. The magnitude of the stress response depended on the stress factor, cultivation conditions as well as the rate of change of the stress factor. The rate of Hsp12p synthesis increased due to all applied stresses, with the observed increase between 2 and 20 times lower when the stress was applied gradually rather than rapidly. The results suggested that the Hsp12p expression rate is a good indicator of applied stress in S. cerevisiae. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/18625028/The_response_of_the_yeast_Saccharomyces_cerevisiae_to_sudden_vs__gradual_changes_in_environmental_stress_monitored_by_expression_of_the_stress_response_protein_Hsp12p_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2008.00391.x DB - PRIME DP - Unbound Medicine ER -