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Regulation of soluble and membrane-bound trehalase activity and expression of the enzyme in the larval midgut of the bamboo borer Omphisa fuscidentalis.
Insect Biochem Mol Biol. 2008 Aug; 38(8):788-95.IB

Abstract

Diapausing larvae of Omphisa fuscidentalis contain soluble and membrane-bound trehalase in the midgut. Soluble trehalase activity accounts for three-fourths of the total trehalase activity in midgut homogenates. The exposure of diapausing larvae to juvenile hormone analog (JHA) induced pupation, accompanied by an increase in soluble trehalase activity at the beginning of the prepupal period. Injection of 20-hydroxyecdysone (20E) increased the level of soluble trehalase activity 5 days postinjection in a dose-dependent manner. In contrast, no increase in membrane-bound trehalase activity was observed under the same conditions. We cloned the cDNAs that encode the soluble and membrane-bound forms of trehalase in O. fuscidentalis trehalase-1 (OfTreh-1) and trehalase-2 (OfTreh-2), respectively. Treh-1 encodes a 581-aa protein while Treh-2 encodes a 648-aa protein with one putative transmembrane domain near the C-terminus. The mRNA expression level of Treh-1 was 27-fold higher than that of Treh-2 in diapausing larval midgut. Following the exposure of diapausing larvae to JHA, Treh-1 mRNA expression increased gradually until the prepupal period whereupon it increased dramatically; in contrast, the mRNA expression of Treh-2 remained at its initial level. Similarly, 20E upregulated Treh-1 expression but had no effect on Treh-2 expression. Taken together, these results suggest that an increase in the soluble trehalase activity at pupation is caused by upregulation of Treh-1 gene. Moreover, membrane-bound trehalase does not appear to be involved in the dynamic changes in the hemolymph trehalose concentration that occur during the larval-pupal transformation.

Authors+Show Affiliations

Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18625402

Citation

Tatun, Nujira, et al. "Regulation of Soluble and Membrane-bound Trehalase Activity and Expression of the Enzyme in the Larval Midgut of the Bamboo Borer Omphisa Fuscidentalis." Insect Biochemistry and Molecular Biology, vol. 38, no. 8, 2008, pp. 788-95.
Tatun N, Singtripop T, Tungjitwitayakul J, et al. Regulation of soluble and membrane-bound trehalase activity and expression of the enzyme in the larval midgut of the bamboo borer Omphisa fuscidentalis. Insect Biochem Mol Biol. 2008;38(8):788-95.
Tatun, N., Singtripop, T., Tungjitwitayakul, J., & Sakurai, S. (2008). Regulation of soluble and membrane-bound trehalase activity and expression of the enzyme in the larval midgut of the bamboo borer Omphisa fuscidentalis. Insect Biochemistry and Molecular Biology, 38(8), 788-95. https://doi.org/10.1016/j.ibmb.2008.05.003
Tatun N, et al. Regulation of Soluble and Membrane-bound Trehalase Activity and Expression of the Enzyme in the Larval Midgut of the Bamboo Borer Omphisa Fuscidentalis. Insect Biochem Mol Biol. 2008;38(8):788-95. PubMed PMID: 18625402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of soluble and membrane-bound trehalase activity and expression of the enzyme in the larval midgut of the bamboo borer Omphisa fuscidentalis. AU - Tatun,Nujira, AU - Singtripop,Tippawan, AU - Tungjitwitayakul,Jatuporn, AU - Sakurai,Sho, Y1 - 2008/05/22/ PY - 2008/01/30/received PY - 2008/04/14/revised PY - 2008/05/15/accepted PY - 2008/7/16/pubmed PY - 2008/10/15/medline PY - 2008/7/16/entrez SP - 788 EP - 95 JF - Insect biochemistry and molecular biology JO - Insect Biochem. Mol. Biol. VL - 38 IS - 8 N2 - Diapausing larvae of Omphisa fuscidentalis contain soluble and membrane-bound trehalase in the midgut. Soluble trehalase activity accounts for three-fourths of the total trehalase activity in midgut homogenates. The exposure of diapausing larvae to juvenile hormone analog (JHA) induced pupation, accompanied by an increase in soluble trehalase activity at the beginning of the prepupal period. Injection of 20-hydroxyecdysone (20E) increased the level of soluble trehalase activity 5 days postinjection in a dose-dependent manner. In contrast, no increase in membrane-bound trehalase activity was observed under the same conditions. We cloned the cDNAs that encode the soluble and membrane-bound forms of trehalase in O. fuscidentalis trehalase-1 (OfTreh-1) and trehalase-2 (OfTreh-2), respectively. Treh-1 encodes a 581-aa protein while Treh-2 encodes a 648-aa protein with one putative transmembrane domain near the C-terminus. The mRNA expression level of Treh-1 was 27-fold higher than that of Treh-2 in diapausing larval midgut. Following the exposure of diapausing larvae to JHA, Treh-1 mRNA expression increased gradually until the prepupal period whereupon it increased dramatically; in contrast, the mRNA expression of Treh-2 remained at its initial level. Similarly, 20E upregulated Treh-1 expression but had no effect on Treh-2 expression. Taken together, these results suggest that an increase in the soluble trehalase activity at pupation is caused by upregulation of Treh-1 gene. Moreover, membrane-bound trehalase does not appear to be involved in the dynamic changes in the hemolymph trehalose concentration that occur during the larval-pupal transformation. SN - 0965-1748 UR - https://www.unboundmedicine.com/medline/citation/18625402/Regulation_of_soluble_and_membrane_bound_trehalase_activity_and_expression_of_the_enzyme_in_the_larval_midgut_of_the_bamboo_borer_Omphisa_fuscidentalis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(08)00087-8 DB - PRIME DP - Unbound Medicine ER -