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[Observation on the accumulative analgesic effect of electroacupuncture and the expression of protein kinase A in hypothalamus and hippocampus in chronic pain or/and ovariectomized rats].
Zhen Ci Yan Jiu. 2008 Apr; 33(2):80-7.ZC

Abstract

OBJECTIVE

To observe the accumulative analgesic effect of electroacupuncture (EA) and the simultaneous changes of protein kinase A (PKA) expression in hypothalamus and hippocampus in rats with chronic constrictive injury (CCI) or/and learning-memory impairment.

METHODS

Sixty-eight female Wistar rats were randomized into normal control, CCI, CCI + EA 2 d, CCI + EA 2 w, OVX + CCI, OVX + CCI + EA 2 d and OVX + CCI + EA 2 w groups, with 10 cases in each except OVX + CCI group (n=8). CCI pain model was established by ligature of the right sciatic nerve with surgical suture. EA (2/15 Hz, 1-2 mA, 30 min) was daily applied to bilateral "Zusanli" (ST 36) and "Yanglingquan" (GB 34) for 2 days (2 d) and 2 weeks (2 w) respectively. Neuro-memory impairment was induced by OVX plus subcutaneous injection of D-galactose. Hyperalgesic scores (HS) were detected with radiation-heat irradiation and PKA activity was detected by immunohistochemical method.

RESULTS

After CCI, HS of all groups increased significantly in comparison with normal control group (P<0.05). In simple CCI rats, compared with CCI group, HS of both CCI + EA 2 d and CCI + EA 2 w groups decreased markedly (P<0.05), and that of CCI + EA 2 w group was evidently lower than that of CCI + EA 2 d group on the 18th day after CCI (P<0.05). In memory impairment rats, HS of both OVX + CCI + EA 2 d and OVX + CCI + EA 2 w groups was significantly lower than that of OVX+ CCI group (P<0.05); and no significant difference was found between OVX + CCI + EA 2 w and OVX + CCI + EA 2 d groups in HS (P>0.05). Results of immunohistochemistry showed that the integral grey values (IGV) of PKA of paraventricular nucleus (PVN), arcuate nucleus (ARC) and supraoptic nucleus (SON) of hypothalamus in CCI + EA 2 w group were significantly lower than those in normal control and CCI + EA 2 d groups (P<0.05). IGV of PVN and SON in OVX + CCI + EA 2 w group were also markedly lower than those in OVX + CCI group (P<0.05), displaying upregulation and cumulative increase of PKA expression by EA 2 w. IGV of PKA, ARC and SON in OVX + CCI + EA 2 w group were markedly higher than those in CCI + EA 2 w group (P<0.05), suggesting an apparent attenuation of the cumulative upregulation effect of PKA expression after learning-memory injury. The changing tendency of PKA expression in hippocampal CA1 region was similar to that of SON after repeated EA of ST36 and GB34.

CONCLUSION

Repeated EA has a cumulative analgesic effect in CCI rats, which is probably related to its resultant upregulation of PKA expression in neurons of hypothalamus and hippocampus and the animals' neuro-memory.

Authors+Show Affiliations

Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18630581

Citation

Wang, Jun-Ying, et al. "[Observation On the Accumulative Analgesic Effect of Electroacupuncture and the Expression of Protein Kinase a in Hypothalamus and Hippocampus in Chronic Pain Or/and Ovariectomized Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 33, no. 2, 2008, pp. 80-7.
Wang JY, Chen SP, Li YH, et al. [Observation on the accumulative analgesic effect of electroacupuncture and the expression of protein kinase A in hypothalamus and hippocampus in chronic pain or/and ovariectomized rats]. Zhen Ci Yan Jiu. 2008;33(2):80-7.
Wang, J. Y., Chen, S. P., Li, Y. H., Meng, F. Y., Gao, Y. H., & Liu, J. L. (2008). [Observation on the accumulative analgesic effect of electroacupuncture and the expression of protein kinase A in hypothalamus and hippocampus in chronic pain or/and ovariectomized rats]. Zhen Ci Yan Jiu = Acupuncture Research, 33(2), 80-7.
Wang JY, et al. [Observation On the Accumulative Analgesic Effect of Electroacupuncture and the Expression of Protein Kinase a in Hypothalamus and Hippocampus in Chronic Pain Or/and Ovariectomized Rats]. Zhen Ci Yan Jiu. 2008;33(2):80-7. PubMed PMID: 18630581.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Observation on the accumulative analgesic effect of electroacupuncture and the expression of protein kinase A in hypothalamus and hippocampus in chronic pain or/and ovariectomized rats]. AU - Wang,Jun-Ying, AU - Chen,Shu-Ping, AU - Li,Yan-Hua, AU - Meng,Fan-Ying, AU - Gao,Yong-Hui, AU - Liu,Jun-Ling, PY - 2008/7/18/pubmed PY - 2008/7/30/medline PY - 2008/7/18/entrez SP - 80 EP - 7 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 33 IS - 2 N2 - OBJECTIVE: To observe the accumulative analgesic effect of electroacupuncture (EA) and the simultaneous changes of protein kinase A (PKA) expression in hypothalamus and hippocampus in rats with chronic constrictive injury (CCI) or/and learning-memory impairment. METHODS: Sixty-eight female Wistar rats were randomized into normal control, CCI, CCI + EA 2 d, CCI + EA 2 w, OVX + CCI, OVX + CCI + EA 2 d and OVX + CCI + EA 2 w groups, with 10 cases in each except OVX + CCI group (n=8). CCI pain model was established by ligature of the right sciatic nerve with surgical suture. EA (2/15 Hz, 1-2 mA, 30 min) was daily applied to bilateral "Zusanli" (ST 36) and "Yanglingquan" (GB 34) for 2 days (2 d) and 2 weeks (2 w) respectively. Neuro-memory impairment was induced by OVX plus subcutaneous injection of D-galactose. Hyperalgesic scores (HS) were detected with radiation-heat irradiation and PKA activity was detected by immunohistochemical method. RESULTS: After CCI, HS of all groups increased significantly in comparison with normal control group (P<0.05). In simple CCI rats, compared with CCI group, HS of both CCI + EA 2 d and CCI + EA 2 w groups decreased markedly (P<0.05), and that of CCI + EA 2 w group was evidently lower than that of CCI + EA 2 d group on the 18th day after CCI (P<0.05). In memory impairment rats, HS of both OVX + CCI + EA 2 d and OVX + CCI + EA 2 w groups was significantly lower than that of OVX+ CCI group (P<0.05); and no significant difference was found between OVX + CCI + EA 2 w and OVX + CCI + EA 2 d groups in HS (P>0.05). Results of immunohistochemistry showed that the integral grey values (IGV) of PKA of paraventricular nucleus (PVN), arcuate nucleus (ARC) and supraoptic nucleus (SON) of hypothalamus in CCI + EA 2 w group were significantly lower than those in normal control and CCI + EA 2 d groups (P<0.05). IGV of PVN and SON in OVX + CCI + EA 2 w group were also markedly lower than those in OVX + CCI group (P<0.05), displaying upregulation and cumulative increase of PKA expression by EA 2 w. IGV of PKA, ARC and SON in OVX + CCI + EA 2 w group were markedly higher than those in CCI + EA 2 w group (P<0.05), suggesting an apparent attenuation of the cumulative upregulation effect of PKA expression after learning-memory injury. The changing tendency of PKA expression in hippocampal CA1 region was similar to that of SON after repeated EA of ST36 and GB34. CONCLUSION: Repeated EA has a cumulative analgesic effect in CCI rats, which is probably related to its resultant upregulation of PKA expression in neurons of hypothalamus and hippocampus and the animals' neuro-memory. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/18630581/[Observation_on_the_accumulative_analgesic_effect_of_electroacupuncture_and_the_expression_of_protein_kinase_A_in_hypothalamus_and_hippocampus_in_chronic_pain_or/and_ovariectomized_rats]_ L2 - https://medlineplus.gov/pain.html DB - PRIME DP - Unbound Medicine ER -