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A unique caleosin in oil bodies of lily pollen.
Plant Cell Physiol. 2008 Sep; 49(9):1390-5.PC

Abstract

In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.

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Authors+Show Affiliations

Jiang PL
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.
Jauh GY
No affiliation info available
Wang CS
No affiliation info available
Tzen JT
No affiliation info available

MeSH

Amino Acid SequenceCalcium-Binding ProteinsCloning, MolecularElectrophoresis, Polyacrylamide GelGenes, PlantLiliumMolecular Sequence DataPhylogenyPlant OilsPlant ProteinsPollenRNA, PlantSequence AlignmentSequence Homology, Amino AcidSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18632804

Citation

Jiang, Pei-Luen, et al. "A Unique Caleosin in Oil Bodies of Lily Pollen." Plant & Cell Physiology, vol. 49, no. 9, 2008, pp. 1390-5.
Jiang PL, Jauh GY, Wang CS, et al. A unique caleosin in oil bodies of lily pollen. Plant Cell Physiol. 2008;49(9):1390-5.
Jiang, P. L., Jauh, G. Y., Wang, C. S., & Tzen, J. T. (2008). A unique caleosin in oil bodies of lily pollen. Plant & Cell Physiology, 49(9), 1390-5. https://doi.org/10.1093/pcp/pcn103
Jiang PL, et al. A Unique Caleosin in Oil Bodies of Lily Pollen. Plant Cell Physiol. 2008;49(9):1390-5. PubMed PMID: 18632804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A unique caleosin in oil bodies of lily pollen. AU - Jiang,Pei-Luen, AU - Jauh,Guang-Yuh, AU - Wang,Co-Shing, AU - Tzen,Jason T C, Y1 - 2008/07/16/ PY - 2008/7/18/pubmed PY - 2008/11/15/medline PY - 2008/7/18/entrez SP - 1390 EP - 5 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 49 IS - 9 N2 - In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/18632804/A_unique_caleosin_in_oil_bodies_of_lily_pollen_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcn103 DB - PRIME DP - Unbound Medicine ER -