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Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2008 Aug; 22(16):2447-52.RC

Abstract

High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MS(n)) was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS(1-3) in positive ion mode. The MS(n) spectra of the [M+H](+) ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-beta-D-quinovopyranoside and solagenin 6-O-[beta-D-xylopyranosyl-(1 --> 3)-O-beta-D-quinovopyranoside].

Authors+Show Affiliations

Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18634112

Citation

Lu, Yuanyuan, et al. "Characterization of Spirostanol Saponins in Solanum Torvum By High-performance Liquid Chromatography/evaporative Light Scattering Detector/electrospray Ionization With Multi-stage Tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 16, 2008, pp. 2447-52.
Lu Y, Luo J, Xu D, et al. Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry. Rapid Commun Mass Spectrom. 2008;22(16):2447-52.
Lu, Y., Luo, J., Xu, D., Huang, X., & Kong, L. (2008). Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 22(16), 2447-52. https://doi.org/10.1002/rcm.3630
Lu Y, et al. Characterization of Spirostanol Saponins in Solanum Torvum By High-performance Liquid Chromatography/evaporative Light Scattering Detector/electrospray Ionization With Multi-stage Tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2008;22(16):2447-52. PubMed PMID: 18634112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry. AU - Lu,Yuanyuan, AU - Luo,Jianguang, AU - Xu,Deran, AU - Huang,Xuefeng, AU - Kong,Lingyi, PY - 2008/7/18/pubmed PY - 2008/9/13/medline PY - 2008/7/18/entrez SP - 2447 EP - 52 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 16 N2 - High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MS(n)) was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS(1-3) in positive ion mode. The MS(n) spectra of the [M+H](+) ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-beta-D-quinovopyranoside and solagenin 6-O-[beta-D-xylopyranosyl-(1 --> 3)-O-beta-D-quinovopyranoside]. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18634112/Characterization_of_spirostanol_saponins_in_Solanum_torvum_by_high_performance_liquid_chromatography/evaporative_light_scattering_detector/electrospray_ionization_with_multi_stage_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3630 DB - PRIME DP - Unbound Medicine ER -