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Evidence that inhibition of spinal nitric oxide production contributes to the antinociceptive effects of emulsified isoflurane on formalin-induced pain in rats.
Clin Exp Pharmacol Physiol. 2008 Oct; 35(10):1245-51.CE

Abstract

The aim of the present study was to investigate the contribution of spinal nitric oxide (NO) to the antinociceptive effects of emulsified isofluane in rats. The formalin test was used to assess nociceptive responses. Immunocytochemistry and histochemistry were performed to determine the effects of emulsified isoflurane on formalin-induced changes in Fos-like immunoreactive (Fos-LI)- and nicotinamide adenine dinucleotide phosphatediaphorase (NADPH-d)-positive neurons, respectively. The results showed that emulsified isofluane, administered intraperitoneally, significantly decreased the formalin-induced paw licking time and that this was attenuated by pretreatment with intrathecal injection of the NO precursor L-arginine. Furthermore, Fos-LI- and NADPH-d-positive neurons were mainly found in the ipsilateral dorsal horn after injection of formalin, some of which were Fos-LI/NADPH-d double-labelled neurons. Administration of emulsified isofluane significantly decreased Fos-LI- and NADPH-d-positive, as well as Fos-LI/NADPH-d double-labelled, neurons. Finally, emulsified isofluane produced a significant reduction of NOS activity and a decrease of NO production in the spinal cord of formalin-treated rats. In conclusion, the results suggest that inhibition of spinal NO production contributes to the antinociceptive effects of emulsified isofluane on formalin-induced pain in rats.

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Authors+Show Affiliations

Ge ZJ
Department of Anaesthesiology, Affiliated Hospital of Jiangsu University, Yixing People's Hospital, Yixing City, Jiangsu, China.
Tan YF
No affiliation info available
Zhao YP
No affiliation info available
Cui GX
No affiliation info available

MeSH

AnalgesicsAnimalsEmulsifying AgentsFemaleInjections, SpinalIsofluraneMaleNitric OxidePainPain MeasurementRatsRats, Sprague-DawleySpinal Cord

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18637015

Citation

Ge, Zhi-Jun, et al. "Evidence That Inhibition of Spinal Nitric Oxide Production Contributes to the Antinociceptive Effects of Emulsified Isoflurane On Formalin-induced Pain in Rats." Clinical and Experimental Pharmacology & Physiology, vol. 35, no. 10, 2008, pp. 1245-51.
Ge ZJ, Tan YF, Zhao YP, et al. Evidence that inhibition of spinal nitric oxide production contributes to the antinociceptive effects of emulsified isoflurane on formalin-induced pain in rats. Clin Exp Pharmacol Physiol. 2008;35(10):1245-51.
Ge, Z. J., Tan, Y. F., Zhao, Y. P., & Cui, G. X. (2008). Evidence that inhibition of spinal nitric oxide production contributes to the antinociceptive effects of emulsified isoflurane on formalin-induced pain in rats. Clinical and Experimental Pharmacology & Physiology, 35(10), 1245-51. https://doi.org/10.1111/j.1440-1681.2008.05001.x
Ge ZJ, et al. Evidence That Inhibition of Spinal Nitric Oxide Production Contributes to the Antinociceptive Effects of Emulsified Isoflurane On Formalin-induced Pain in Rats. Clin Exp Pharmacol Physiol. 2008;35(10):1245-51. PubMed PMID: 18637015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence that inhibition of spinal nitric oxide production contributes to the antinociceptive effects of emulsified isoflurane on formalin-induced pain in rats. AU - Ge,Zhi-Jun, AU - Tan,Yong-Fei, AU - Zhao,Yan-Ping, AU - Cui,Guo-Xing, Y1 - 2008/07/09/ PY - 2008/7/19/pubmed PY - 2009/6/20/medline PY - 2008/7/19/entrez SP - 1245 EP - 51 JF - Clinical and experimental pharmacology & physiology JO - Clin Exp Pharmacol Physiol VL - 35 IS - 10 N2 - The aim of the present study was to investigate the contribution of spinal nitric oxide (NO) to the antinociceptive effects of emulsified isofluane in rats. The formalin test was used to assess nociceptive responses. Immunocytochemistry and histochemistry were performed to determine the effects of emulsified isoflurane on formalin-induced changes in Fos-like immunoreactive (Fos-LI)- and nicotinamide adenine dinucleotide phosphatediaphorase (NADPH-d)-positive neurons, respectively. The results showed that emulsified isofluane, administered intraperitoneally, significantly decreased the formalin-induced paw licking time and that this was attenuated by pretreatment with intrathecal injection of the NO precursor L-arginine. Furthermore, Fos-LI- and NADPH-d-positive neurons were mainly found in the ipsilateral dorsal horn after injection of formalin, some of which were Fos-LI/NADPH-d double-labelled neurons. Administration of emulsified isofluane significantly decreased Fos-LI- and NADPH-d-positive, as well as Fos-LI/NADPH-d double-labelled, neurons. Finally, emulsified isofluane produced a significant reduction of NOS activity and a decrease of NO production in the spinal cord of formalin-treated rats. In conclusion, the results suggest that inhibition of spinal NO production contributes to the antinociceptive effects of emulsified isofluane on formalin-induced pain in rats. SN - 1440-1681 UR - https://www.unboundmedicine.com/medline/citation/18637015/Evidence_that_inhibition_of_spinal_nitric_oxide_production_contributes_to_the_antinociceptive_effects_of_emulsified_isoflurane_on_formalin_induced_pain_in_rats_ L2 - https://doi.org/10.1111/j.1440-1681.2008.05001.x DB - PRIME DP - Unbound Medicine ER -