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[Expression Fas and FasL antigen protein of lymphocytes T and B in hypertrophied adenoid in children with otitis media with effusion].
Otolaryngol Pol. 2008; 62(1):59-64.OP

Abstract

INTRODUCTION

Apoptosis of lymphocytes can be induced by different factors mainly however the activation of antigen Fas (CD95) and his ligand FasL (CD95L) route. Apoptosis induced by Fas-FasL has large significance in elimination of lymphocytes T in final phase immune response designate AICD (activation induced cell death). Lymphocytes B undergo apoptosis induced by Fas-FasL in germinal center, which has special meaning in elimination of cells about low specificity and weak affinity of receptor BCR (B-cell antigen receptor).

AIM OF STUDY

The aim of this study was evaluation of percentage of apoptotic lymphocytes and expression of Fas and FasL in CD4+, CD8+, CD19+ lymphocytes in hypertrophied adenoid and hypertrophied adenoid and otitis media with effusion.

METHODS

CD4+Fas+, CD8+Fas+, CD19+Fas+CD4+FasL+, CD8+FasL+, CD19+FasL+ cells subpopulation were identified using monoclonal antibodies and flow cytometry method.

RESULTS

The percentage of lymphocytes CD4+ Fas+, CD8+Fas+, CD19+Fas+ was higher in hypertrophied adenoid and otitis media with effusion compored to the control group. Their was no significant difference the percentage CD4+ Fas+, CD8+Fas+, CD19+Fas+CD4+FasL+, CD8+FasL+, CD19+FasL+ in hypertrophied adenoid and otitis media with effusion then the control group. The percentage of apoptotic lymphocytes was higher in hypertrophied adenoid and otitis media with effusion compored to the control group.

CONCLUSIONS

The susceptibility the individual subpopulation of lymphocytes in hypertrophied adenoid seems to influence on change of relations of quantitative lymphocytes and upset the immunological function of tonsils which can influence on course otitis media with effusion at children.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zelazowska-Rutkowska B
Zakład Laboratoryjnej Diagnostyki Pediatrycznej AM w Białymstoku. zelazowskab@wp.pl
Wysocka J
No affiliation info available
Ratomski K
No affiliation info available
Skotnicka B
No affiliation info available
Zak J
No affiliation info available
Mrugacz M
No affiliation info available

MeSH

AdenoidectomyAdenoidsAdolescentAntigens, CDApoptosisB-LymphocytesChildChild, PreschoolFas Ligand ProteinFemaleFlow CytometryHumansHypertrophyInfantLymphocyte CountMaleOtitis Media with EffusionT-Lymphocytesfas Receptor

Pub Type(s)

Journal Article

Language

pol

PubMed ID

18637423

Citation

Zelazowska-Rutkowska, Beata, et al. "[Expression Fas and FasL Antigen Protein of Lymphocytes T and B in Hypertrophied Adenoid in Children With Otitis Media With Effusion]." Otolaryngologia Polska = the Polish Otolaryngology, vol. 62, no. 1, 2008, pp. 59-64.
Zelazowska-Rutkowska B, Wysocka J, Ratomski K, et al. [Expression Fas and FasL antigen protein of lymphocytes T and B in hypertrophied adenoid in children with otitis media with effusion]. Otolaryngol Pol. 2008;62(1):59-64.
Zelazowska-Rutkowska, B., Wysocka, J., Ratomski, K., Skotnicka, B., Zak, J., & Mrugacz, M. (2008). [Expression Fas and FasL antigen protein of lymphocytes T and B in hypertrophied adenoid in children with otitis media with effusion]. Otolaryngologia Polska = the Polish Otolaryngology, 62(1), 59-64. https://doi.org/10.1016/S0030-6657(08)70210-5
Zelazowska-Rutkowska B, et al. [Expression Fas and FasL Antigen Protein of Lymphocytes T and B in Hypertrophied Adenoid in Children With Otitis Media With Effusion]. Otolaryngol Pol. 2008;62(1):59-64. PubMed PMID: 18637423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression Fas and FasL antigen protein of lymphocytes T and B in hypertrophied adenoid in children with otitis media with effusion]. AU - Zelazowska-Rutkowska,Beata, AU - Wysocka,Jolanta, AU - Ratomski,Karol, AU - Skotnicka,Bozena, AU - Zak,Janusz, AU - Mrugacz,Małgorzata, PY - 2008/7/22/pubmed PY - 2008/9/24/medline PY - 2008/7/22/entrez SP - 59 EP - 64 JF - Otolaryngologia polska = The Polish otolaryngology JO - Otolaryngol Pol VL - 62 IS - 1 N2 - INTRODUCTION: Apoptosis of lymphocytes can be induced by different factors mainly however the activation of antigen Fas (CD95) and his ligand FasL (CD95L) route. Apoptosis induced by Fas-FasL has large significance in elimination of lymphocytes T in final phase immune response designate AICD (activation induced cell death). Lymphocytes B undergo apoptosis induced by Fas-FasL in germinal center, which has special meaning in elimination of cells about low specificity and weak affinity of receptor BCR (B-cell antigen receptor). AIM OF STUDY: The aim of this study was evaluation of percentage of apoptotic lymphocytes and expression of Fas and FasL in CD4+, CD8+, CD19+ lymphocytes in hypertrophied adenoid and hypertrophied adenoid and otitis media with effusion. METHODS: CD4+Fas+, CD8+Fas+, CD19+Fas+CD4+FasL+, CD8+FasL+, CD19+FasL+ cells subpopulation were identified using monoclonal antibodies and flow cytometry method. RESULTS: The percentage of lymphocytes CD4+ Fas+, CD8+Fas+, CD19+Fas+ was higher in hypertrophied adenoid and otitis media with effusion compored to the control group. Their was no significant difference the percentage CD4+ Fas+, CD8+Fas+, CD19+Fas+CD4+FasL+, CD8+FasL+, CD19+FasL+ in hypertrophied adenoid and otitis media with effusion then the control group. The percentage of apoptotic lymphocytes was higher in hypertrophied adenoid and otitis media with effusion compored to the control group. CONCLUSIONS: The susceptibility the individual subpopulation of lymphocytes in hypertrophied adenoid seems to influence on change of relations of quantitative lymphocytes and upset the immunological function of tonsils which can influence on course otitis media with effusion at children. SN - 0030-6657 UR - https://www.unboundmedicine.com/medline/citation/18637423/[Expression_Fas_and_FasL_antigen_protein_of_lymphocytes_T_and_B_in_hypertrophied_adenoid_in_children_with_otitis_media_with_effusion]_ DB - PRIME DP - Unbound Medicine ER -