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Melatonin-evoked in vivo secretion of protein and amylase from the parotid gland of the anaesthetised rat.
J Pineal Res. 2008 Nov; 45(4):413-21.JP

Abstract

The intravenous infusion of melatonin (5 and 25 mg/kg over 10 min) evoked a dose-dependent output of protein and amylase but no overt fluid secretion from the parotid gland of the pentobarbitone-anaesthetised rat, as revealed by increased concentrations of protein and amylase activity in a subsequent wash-out flow of saliva in response to an intravenous bolus injection of methacholine (5 microg/kg) 10 min later. The secretory responses to melatonin occurred in the presence of alpha- and beta-adrenoceptor antagonists. They were not affected by the cholecystokinin A-receptor antagonist, lorglumide, and they were reproduced in eviscerated animals acutely subjected to postganglionic sympathetic and parasympathetic denervation of the gland. The responses to melatonin were partially dependent on nitric oxide generation, through the activity of nitric oxide synthase of the neuronal type. Immunoblotting showed both melatonin receptors of type 1 and type 2 to be expressed in parotid gland tissue. The relative specific melatonin 2-receptor antagonist luzindole prevented the expected secretory effects of melatonin. The results favour a direct action by melatonin on melatonin receptors of parotid secretory cells and suggest a potential physiological role for melatonin in the regulation of salivary glandular activities.

Authors+Show Affiliations

Section of Pharmacology, Institute of Neuroscience and Physiology, the Sahlgrenska Academy at Göteborg University, Göteborg, Sweden.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18637985

Citation

Aras, Hülya Cevik, and Jörgen Ekström. "Melatonin-evoked in Vivo Secretion of Protein and Amylase From the Parotid Gland of the Anaesthetised Rat." Journal of Pineal Research, vol. 45, no. 4, 2008, pp. 413-21.
Aras HC, Ekström J. Melatonin-evoked in vivo secretion of protein and amylase from the parotid gland of the anaesthetised rat. J Pineal Res. 2008;45(4):413-21.
Aras, H. C., & Ekström, J. (2008). Melatonin-evoked in vivo secretion of protein and amylase from the parotid gland of the anaesthetised rat. Journal of Pineal Research, 45(4), 413-21. https://doi.org/10.1111/j.1600-079X.2008.00609.x
Aras HC, Ekström J. Melatonin-evoked in Vivo Secretion of Protein and Amylase From the Parotid Gland of the Anaesthetised Rat. J Pineal Res. 2008;45(4):413-21. PubMed PMID: 18637985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melatonin-evoked in vivo secretion of protein and amylase from the parotid gland of the anaesthetised rat. AU - Aras,Hülya Cevik, AU - Ekström,Jörgen, Y1 - 2008/07/10/ PY - 2008/7/22/pubmed PY - 2008/12/25/medline PY - 2008/7/22/entrez SP - 413 EP - 21 JF - Journal of pineal research JO - J Pineal Res VL - 45 IS - 4 N2 - The intravenous infusion of melatonin (5 and 25 mg/kg over 10 min) evoked a dose-dependent output of protein and amylase but no overt fluid secretion from the parotid gland of the pentobarbitone-anaesthetised rat, as revealed by increased concentrations of protein and amylase activity in a subsequent wash-out flow of saliva in response to an intravenous bolus injection of methacholine (5 microg/kg) 10 min later. The secretory responses to melatonin occurred in the presence of alpha- and beta-adrenoceptor antagonists. They were not affected by the cholecystokinin A-receptor antagonist, lorglumide, and they were reproduced in eviscerated animals acutely subjected to postganglionic sympathetic and parasympathetic denervation of the gland. The responses to melatonin were partially dependent on nitric oxide generation, through the activity of nitric oxide synthase of the neuronal type. Immunoblotting showed both melatonin receptors of type 1 and type 2 to be expressed in parotid gland tissue. The relative specific melatonin 2-receptor antagonist luzindole prevented the expected secretory effects of melatonin. The results favour a direct action by melatonin on melatonin receptors of parotid secretory cells and suggest a potential physiological role for melatonin in the regulation of salivary glandular activities. SN - 1600-079X UR - https://www.unboundmedicine.com/medline/citation/18637985/Melatonin_evoked_in_vivo_secretion_of_protein_and_amylase_from_the_parotid_gland_of_the_anaesthetised_rat_ DB - PRIME DP - Unbound Medicine ER -