Tags

Type your tag names separated by a space and hit enter

Bioassay-guided isolation of anti-inflammatory and antinociceptive principles from a folk remedy, Rhododendron ponticum L. leaves.
J Ethnopharmacol. 2008 Sep 02; 119(1):172-8.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Rhododendron ponticum L. (Ericaceae) is used for the treatment of inflammatory diseases and to alleviate rheumatic pain and against toothache in Turkish traditional medicine.

AIM OF THE STUDY

To evaluate the anti-inflammatory and antinociceptive effects of Rhododendron ponticum leaves using in vivo models, and isolation and chemical characterization of the biologically active constituents through bioassay-guided fractionation procedures.

MATERIAL AND METHODS

Carrageenan-induced hind paw edema model was used for anti-inflammatory activity and p-benzoquinone induced abdominal contractions model for the antinociceptive activity assessment.

RESULTS

The ethylacetate fraction displayed marked anti-inflammatory (28.4-40.7% inhibition) and antinociceptive (50.7% inhibition) effects as compared to reference compounds. Through bioassay-guided fractionation and isolation procedures flavonol glycosides [a mixture of hyperoside and isoquercitrin (1) and quercitrin (2)] along with one flavanone glycoside [6-C-glycosylnaringenin (3)] were isolated as the active ingredients of ethylacetate extract against carrageenan-induced edema and p-benzoquinone-induced writhes and their structures were elucidated by spectral techniques. 1 and 2 also showed a significant anti-inflammatory activity against 12-O-tetradecanoyl-13-acetate (TPA)- induced mouse ear edema model.

CONCLUSION

Results of the present study supported the utilization of the plant in Turkish folk medicine and revealed that flavones are the major anti-inflammatory and antinociceptive principles of the leaves.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18638535

Citation

Erdemoglu, Nurgun, et al. "Bioassay-guided Isolation of Anti-inflammatory and Antinociceptive Principles From a Folk Remedy, Rhododendron Ponticum L. Leaves." Journal of Ethnopharmacology, vol. 119, no. 1, 2008, pp. 172-8.
Erdemoglu N, Akkol EK, Yesilada E, et al. Bioassay-guided isolation of anti-inflammatory and antinociceptive principles from a folk remedy, Rhododendron ponticum L. leaves. J Ethnopharmacol. 2008;119(1):172-8.
Erdemoglu, N., Akkol, E. K., Yesilada, E., & Caliş, I. (2008). Bioassay-guided isolation of anti-inflammatory and antinociceptive principles from a folk remedy, Rhododendron ponticum L. leaves. Journal of Ethnopharmacology, 119(1), 172-8. https://doi.org/10.1016/j.jep.2008.06.021
Erdemoglu N, et al. Bioassay-guided Isolation of Anti-inflammatory and Antinociceptive Principles From a Folk Remedy, Rhododendron Ponticum L. Leaves. J Ethnopharmacol. 2008 Sep 2;119(1):172-8. PubMed PMID: 18638535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioassay-guided isolation of anti-inflammatory and antinociceptive principles from a folk remedy, Rhododendron ponticum L. leaves. AU - Erdemoglu,Nurgun, AU - Akkol,Esra Küpeli, AU - Yesilada,Erdem, AU - Caliş,Ihsan, Y1 - 2008/06/28/ PY - 2007/10/16/received PY - 2008/06/04/revised PY - 2008/06/19/accepted PY - 2008/7/22/pubmed PY - 2009/1/22/medline PY - 2008/7/22/entrez SP - 172 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 119 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Rhododendron ponticum L. (Ericaceae) is used for the treatment of inflammatory diseases and to alleviate rheumatic pain and against toothache in Turkish traditional medicine. AIM OF THE STUDY: To evaluate the anti-inflammatory and antinociceptive effects of Rhododendron ponticum leaves using in vivo models, and isolation and chemical characterization of the biologically active constituents through bioassay-guided fractionation procedures. MATERIAL AND METHODS: Carrageenan-induced hind paw edema model was used for anti-inflammatory activity and p-benzoquinone induced abdominal contractions model for the antinociceptive activity assessment. RESULTS: The ethylacetate fraction displayed marked anti-inflammatory (28.4-40.7% inhibition) and antinociceptive (50.7% inhibition) effects as compared to reference compounds. Through bioassay-guided fractionation and isolation procedures flavonol glycosides [a mixture of hyperoside and isoquercitrin (1) and quercitrin (2)] along with one flavanone glycoside [6-C-glycosylnaringenin (3)] were isolated as the active ingredients of ethylacetate extract against carrageenan-induced edema and p-benzoquinone-induced writhes and their structures were elucidated by spectral techniques. 1 and 2 also showed a significant anti-inflammatory activity against 12-O-tetradecanoyl-13-acetate (TPA)- induced mouse ear edema model. CONCLUSION: Results of the present study supported the utilization of the plant in Turkish folk medicine and revealed that flavones are the major anti-inflammatory and antinociceptive principles of the leaves. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/18638535/Bioassay_guided_isolation_of_anti_inflammatory_and_antinociceptive_principles_from_a_folk_remedy_Rhododendron_ponticum_L__leaves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(08)00339-5 DB - PRIME DP - Unbound Medicine ER -