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Nr4a2 is essential for the differentiation of dopaminergic neurons during zebrafish embryogenesis.
Mol Cell Neurosci. 2008 Oct; 39(2):202-10.MC

Abstract

Nr4a2 is a member of the orphan nuclear receptor gene superfamily, which has been found to be critical for the development and maintenance of mesencephalic dopaminergic (DA) neurons. To uncover the molecular mechanisms by which Nr4a2 contributes to the development of DA neurons, we have applied zebrafish to study the topographic distribution of nr4a2b transcripts, as well as its correlation with neuronal progenitor marker (neurogenin 1) and DA neuron markers (tyrosine hydroxylase, TH and DA transporter, DAT) during neurogenesis. Our studies showed that although nr4a2b transcripts did not co-localize with TH and DAT transcripts in the posterior tuberculum (PT area), knockdown of Nr4a2 resulted in a significant decrease of TH(+) and DAT(+) DA neurons in the PT area, accompanied by a reduction of DA transmitter, which were partially rescued by the injection of mouse Nr4a2 mRNA. Surprisingly, the number of nr4a2b(+) cells in Nr4a2-deficient embryos was increased by 1.6 fold. These results suggest that Nr4a2 may play an important role in the differentiation and maturation rather than the survival of DA progenitors in the PT area during zebrafish early embryogenesis.

Authors+Show Affiliations

Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18638558

Citation

Luo, Guang Rui, et al. "Nr4a2 Is Essential for the Differentiation of Dopaminergic Neurons During Zebrafish Embryogenesis." Molecular and Cellular Neurosciences, vol. 39, no. 2, 2008, pp. 202-10.
Luo GR, Chen Y, Li XP, et al. Nr4a2 is essential for the differentiation of dopaminergic neurons during zebrafish embryogenesis. Mol Cell Neurosci. 2008;39(2):202-10.
Luo, G. R., Chen, Y., Li, X. P., Liu, T. X., & Le, W. D. (2008). Nr4a2 is essential for the differentiation of dopaminergic neurons during zebrafish embryogenesis. Molecular and Cellular Neurosciences, 39(2), 202-10. https://doi.org/10.1016/j.mcn.2008.06.010
Luo GR, et al. Nr4a2 Is Essential for the Differentiation of Dopaminergic Neurons During Zebrafish Embryogenesis. Mol Cell Neurosci. 2008;39(2):202-10. PubMed PMID: 18638558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nr4a2 is essential for the differentiation of dopaminergic neurons during zebrafish embryogenesis. AU - Luo,Guang Rui, AU - Chen,Yi, AU - Li,Xu Ping, AU - Liu,Ting Xi, AU - Le,Wei Dong, Y1 - 2008/06/25/ PY - 2007/09/26/received PY - 2008/06/16/revised PY - 2008/06/17/accepted PY - 2008/7/22/pubmed PY - 2009/4/9/medline PY - 2008/7/22/entrez SP - 202 EP - 10 JF - Molecular and cellular neurosciences JO - Mol. Cell. Neurosci. VL - 39 IS - 2 N2 - Nr4a2 is a member of the orphan nuclear receptor gene superfamily, which has been found to be critical for the development and maintenance of mesencephalic dopaminergic (DA) neurons. To uncover the molecular mechanisms by which Nr4a2 contributes to the development of DA neurons, we have applied zebrafish to study the topographic distribution of nr4a2b transcripts, as well as its correlation with neuronal progenitor marker (neurogenin 1) and DA neuron markers (tyrosine hydroxylase, TH and DA transporter, DAT) during neurogenesis. Our studies showed that although nr4a2b transcripts did not co-localize with TH and DAT transcripts in the posterior tuberculum (PT area), knockdown of Nr4a2 resulted in a significant decrease of TH(+) and DAT(+) DA neurons in the PT area, accompanied by a reduction of DA transmitter, which were partially rescued by the injection of mouse Nr4a2 mRNA. Surprisingly, the number of nr4a2b(+) cells in Nr4a2-deficient embryos was increased by 1.6 fold. These results suggest that Nr4a2 may play an important role in the differentiation and maturation rather than the survival of DA progenitors in the PT area during zebrafish early embryogenesis. SN - 1095-9327 UR - https://www.unboundmedicine.com/medline/citation/18638558/Nr4a2_is_essential_for_the_differentiation_of_dopaminergic_neurons_during_zebrafish_embryogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1044-7431(08)00163-2 DB - PRIME DP - Unbound Medicine ER -