Tags

Type your tag names separated by a space and hit enter

Genotoxic effects of Roundup on the fish Prochilodus lineatus.
Mutat Res. 2008 Aug-Sep; 655(1-2):41-6.MR

Abstract

Glyphosate-based herbicides, such as Roundup, represent the most extensively used herbicides worldwide, including Brazil. Despite its extensive use, the genotoxic effects of this herbicide are not completely understood and studies with Roundup show conflicting results with regard to the effects of this product on the genetic material. Thus, the aim of this study was to evaluate the genotoxic effects of acute exposures (6, 24 and 96 h) to 10 mg L(-1) of Roundup on the neotropical fish Prochilodus lineatus. Accordingly, fish erythrocytes were used in the comet assay, micronucleus test and for the analysis of the occurrence of nuclear abnormalities and the comet assay was adjusted for branchial cells. The results showed that Roundup produces genotoxic damage in erythrocytes and gill cells of P. lineatus. The comet scores obtained for P. lineatus erythrocytes after 6 and 96 h of exposure to Roundup were significantly higher than respective negative controls. For branchial cells comet scores were significantly higher than negative controls after 6 and 24 h exposures. The frequencies of micronucleus and other erythrocyte nuclear abnormalities (ENAs) were not significantly different between Roundup exposed fish and their respective negative controls, for all exposure periods. In conclusion, the results of this work showed that Roundup produced genotoxic effects on the fish species P. lineatus. The comet assay with gill cells showed to be an important complementary tool for detecting genotoxicity, given that it revealed DNA damage in periods of exposure that erythrocytes did not. ENAs frequency was not a good indicator of genotoxicity, but further studies are needed to better understand the origin of these abnormalities.

Authors+Show Affiliations

Departamento de Biologia Geral, Universidade Estadual de Londrina, Paraná, Brazil.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18638566

Citation

Cavalcante, D G S M., et al. "Genotoxic Effects of Roundup On the Fish Prochilodus Lineatus." Mutation Research, vol. 655, no. 1-2, 2008, pp. 41-6.
Cavalcante DG, Martinez CB, Sofia SH. Genotoxic effects of Roundup on the fish Prochilodus lineatus. Mutat Res. 2008;655(1-2):41-6.
Cavalcante, D. G., Martinez, C. B., & Sofia, S. H. (2008). Genotoxic effects of Roundup on the fish Prochilodus lineatus. Mutation Research, 655(1-2), 41-6. https://doi.org/10.1016/j.mrgentox.2008.06.010
Cavalcante DG, Martinez CB, Sofia SH. Genotoxic Effects of Roundup On the Fish Prochilodus Lineatus. Mutat Res. 2008 Aug-Sep;655(1-2):41-6. PubMed PMID: 18638566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic effects of Roundup on the fish Prochilodus lineatus. AU - Cavalcante,D G S M, AU - Martinez,C B R, AU - Sofia,S H, PY - 2008/04/15/received PY - 2008/06/09/revised PY - 2008/06/17/accepted PY - 2008/7/22/pubmed PY - 2008/12/30/medline PY - 2008/7/22/entrez SP - 41 EP - 6 JF - Mutation research JO - Mutat Res VL - 655 IS - 1-2 N2 - Glyphosate-based herbicides, such as Roundup, represent the most extensively used herbicides worldwide, including Brazil. Despite its extensive use, the genotoxic effects of this herbicide are not completely understood and studies with Roundup show conflicting results with regard to the effects of this product on the genetic material. Thus, the aim of this study was to evaluate the genotoxic effects of acute exposures (6, 24 and 96 h) to 10 mg L(-1) of Roundup on the neotropical fish Prochilodus lineatus. Accordingly, fish erythrocytes were used in the comet assay, micronucleus test and for the analysis of the occurrence of nuclear abnormalities and the comet assay was adjusted for branchial cells. The results showed that Roundup produces genotoxic damage in erythrocytes and gill cells of P. lineatus. The comet scores obtained for P. lineatus erythrocytes after 6 and 96 h of exposure to Roundup were significantly higher than respective negative controls. For branchial cells comet scores were significantly higher than negative controls after 6 and 24 h exposures. The frequencies of micronucleus and other erythrocyte nuclear abnormalities (ENAs) were not significantly different between Roundup exposed fish and their respective negative controls, for all exposure periods. In conclusion, the results of this work showed that Roundup produced genotoxic effects on the fish species P. lineatus. The comet assay with gill cells showed to be an important complementary tool for detecting genotoxicity, given that it revealed DNA damage in periods of exposure that erythrocytes did not. ENAs frequency was not a good indicator of genotoxicity, but further studies are needed to better understand the origin of these abnormalities. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/18638566/Genotoxic_effects_of_Roundup_on_the_fish_Prochilodus_lineatus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(08)00179-4 DB - PRIME DP - Unbound Medicine ER -