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Uptake and internalisation of copper by three marine microalgae: comparison of copper-sensitive and copper-tolerant species.
Aquat Toxicol. 2008 Aug 29; 89(2):82-93.AT

Abstract

Although it has been well established that different species of marine algae have different sensitivities to metals, our understanding of the physiological and biochemical basis for these differences is limited. This study investigated copper adsorption and internalisation in three algal species with differing sensitivities to copper. The diatom Phaeodactylum tricornutum was particularly sensitive to copper, with a 72-h IC50 (concentration of copper to inhibit growth rate by 50%) of 8.0 microg Cu L(-1), compared to the green algae Tetraselmis sp. (72-h IC50 47 microg Cu L(-1)) and Dunaliella tertiolecta (72-h IC50 530 microg Cu L(-1)). At these IC50 concentrations, Tetraselmis sp. had much higher intracellular copper (1.97+/-0.01 x 10(-13)g Cu cell(-1)) than P. tricornutum (0.23+/-0.19 x 10(-13)g Cu cell(-1)) and D. tertiolecta (0.59+/-0.05 x 10(-13)g Cu cell(-1)), suggesting that Tetraselmis sp. effectively detoxifies copper within the cell. By contrast, at the same external copper concentration (50 microg L(-1)), D. tertiolecta appears to better exclude copper than Tetraselmis sp. by having a slower copper internalisation rate and lower internal copper concentrations at equivalent extracellular concentrations. The results suggest that the use of internal copper concentrations and net uptake rates alone cannot explain differences in species-sensitivity for different algal species. Model prediction of copper toxicity to marine biota and understanding fundamental differences in species-sensitivity will require, not just an understanding of water quality parameters and copper-cell binding, but also further knowledge of cellular detoxification mechanisms.

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Authors+Show Affiliations

Levy JL
Centre for Environmental Contaminants Research, Land and Water, CSIRO, Private Mail Bag 7, Bangor, New South Wales 2234, Australia. jacqui.levy@gmail.com
Angel BM
No affiliation info available
Stauber JL
No affiliation info available
Poon WL
No affiliation info available
Simpson SL
No affiliation info available
Cheng SH
No affiliation info available
Jolley DF
No affiliation info available

MeSH

ChlorophytaCopperDiatomsMicroscopy, Electron, TransmissionSpecies SpecificityWater Pollutants, Chemical

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18639348

Citation

Levy, Jacqueline L., et al. "Uptake and Internalisation of Copper By Three Marine Microalgae: Comparison of Copper-sensitive and Copper-tolerant Species." Aquatic Toxicology (Amsterdam, Netherlands), vol. 89, no. 2, 2008, pp. 82-93.
Levy JL, Angel BM, Stauber JL, et al. Uptake and internalisation of copper by three marine microalgae: comparison of copper-sensitive and copper-tolerant species. Aquat Toxicol. 2008;89(2):82-93.
Levy, J. L., Angel, B. M., Stauber, J. L., Poon, W. L., Simpson, S. L., Cheng, S. H., & Jolley, D. F. (2008). Uptake and internalisation of copper by three marine microalgae: comparison of copper-sensitive and copper-tolerant species. Aquatic Toxicology (Amsterdam, Netherlands), 89(2), 82-93. https://doi.org/10.1016/j.aquatox.2008.06.003
Levy JL, et al. Uptake and Internalisation of Copper By Three Marine Microalgae: Comparison of Copper-sensitive and Copper-tolerant Species. Aquat Toxicol. 2008 Aug 29;89(2):82-93. PubMed PMID: 18639348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Uptake and internalisation of copper by three marine microalgae: comparison of copper-sensitive and copper-tolerant species. AU - Levy,Jacqueline L, AU - Angel,Brad M, AU - Stauber,Jennifer L, AU - Poon,Wing L, AU - Simpson,Stuart L, AU - Cheng,Shuk Han, AU - Jolley,Dianne F, Y1 - 2008/06/08/ PY - 2008/02/22/received PY - 2008/05/20/revised PY - 2008/06/04/accepted PY - 2008/7/22/pubmed PY - 2009/1/14/medline PY - 2008/7/22/entrez SP - 82 EP - 93 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 89 IS - 2 N2 - Although it has been well established that different species of marine algae have different sensitivities to metals, our understanding of the physiological and biochemical basis for these differences is limited. This study investigated copper adsorption and internalisation in three algal species with differing sensitivities to copper. The diatom Phaeodactylum tricornutum was particularly sensitive to copper, with a 72-h IC50 (concentration of copper to inhibit growth rate by 50%) of 8.0 microg Cu L(-1), compared to the green algae Tetraselmis sp. (72-h IC50 47 microg Cu L(-1)) and Dunaliella tertiolecta (72-h IC50 530 microg Cu L(-1)). At these IC50 concentrations, Tetraselmis sp. had much higher intracellular copper (1.97+/-0.01 x 10(-13)g Cu cell(-1)) than P. tricornutum (0.23+/-0.19 x 10(-13)g Cu cell(-1)) and D. tertiolecta (0.59+/-0.05 x 10(-13)g Cu cell(-1)), suggesting that Tetraselmis sp. effectively detoxifies copper within the cell. By contrast, at the same external copper concentration (50 microg L(-1)), D. tertiolecta appears to better exclude copper than Tetraselmis sp. by having a slower copper internalisation rate and lower internal copper concentrations at equivalent extracellular concentrations. The results suggest that the use of internal copper concentrations and net uptake rates alone cannot explain differences in species-sensitivity for different algal species. Model prediction of copper toxicity to marine biota and understanding fundamental differences in species-sensitivity will require, not just an understanding of water quality parameters and copper-cell binding, but also further knowledge of cellular detoxification mechanisms. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/18639348/Uptake_and_internalisation_of_copper_by_three_marine_microalgae:_comparison_of_copper_sensitive_and_copper_tolerant_species_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(08)00177-X DB - PRIME DP - Unbound Medicine ER -