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Co-circulations of two genotypes of dengue virus in 2006 out-break of dengue hemorrhagic fever in Karachi, Pakistan.
J Clin Virol. 2008 Oct; 43(2):176-9.JC

Abstract

BACKGROUND

The status of dengue genotypes involved in the recent epidemic out-breaks in Pakistan is not well defined.

OBJECTIVES

We sought to analyze the predominant genotype responsible for the most severe and largest out-break of dengue hemorrhagic fever (DHF) that hit Karachi in 2006.

STUDY DESIGN

Retrospective analysis of stored serum samples for dengue virus genotype by multiplex RT-PCR, anti-dengue IgM, IgG and review of clinical charts of patients admitted to Aga Khan University Hospital.

RESULTS

Viral RNA detection of 250 patients revealed positive results in 185 (74.0%) samples. DEN-2 was predominant genotype (n=104, 56.2%) Dengue specific antibodies were detected in 139 samples of which 81 were classified as primary cases. DEN-2 accounted for these. Within secondary cases, 63.2% were due to DEN-2 (total 57), the rest were positive for DEN-3. DHF (p=0.064) and abdominal pain (p=0.059) were more frequently associated with DEN-2 as compared to DEN-3. None of the samples were positive for DEN-1 or DEN-4.

CONCLUSION

Co-circulation of DEN-2 and DEN-3 was responsible for the 2006 out-break in Karachi. Primary and secondary cases were seen in both groups. Cases with DHF showed marginal association with DEN-2. Introduction of a new serotype (DEN-3) and or a genotypic shift of endemic serotype (DEN-2) are the probable factors for the recent out-break of DHF in this region.

Authors+Show Affiliations

Department of Pathology & Microbiology, The Aga Khan University Hospital, Stadium Road, P.O. Box 3500, Karachi 74800, Pakistan. erum.khan@aku.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18639489

Citation

Khan, E, et al. "Co-circulations of Two Genotypes of Dengue Virus in 2006 Out-break of Dengue Hemorrhagic Fever in Karachi, Pakistan." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 43, no. 2, 2008, pp. 176-9.
Khan E, Hasan R, Mehraj V, et al. Co-circulations of two genotypes of dengue virus in 2006 out-break of dengue hemorrhagic fever in Karachi, Pakistan. J Clin Virol. 2008;43(2):176-9.
Khan, E., Hasan, R., Mehraj, V., Nasir, A., Siddiqui, J., & Hewson, R. (2008). Co-circulations of two genotypes of dengue virus in 2006 out-break of dengue hemorrhagic fever in Karachi, Pakistan. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 43(2), 176-9. https://doi.org/10.1016/j.jcv.2008.06.003
Khan E, et al. Co-circulations of Two Genotypes of Dengue Virus in 2006 Out-break of Dengue Hemorrhagic Fever in Karachi, Pakistan. J Clin Virol. 2008;43(2):176-9. PubMed PMID: 18639489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-circulations of two genotypes of dengue virus in 2006 out-break of dengue hemorrhagic fever in Karachi, Pakistan. AU - Khan,E, AU - Hasan,R, AU - Mehraj,V, AU - Nasir,A, AU - Siddiqui,J, AU - Hewson,R, Y1 - 2008/07/18/ PY - 2008/01/25/received PY - 2008/05/13/revised PY - 2008/06/03/accepted PY - 2008/7/22/pubmed PY - 2008/12/17/medline PY - 2008/7/22/entrez SP - 176 EP - 9 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 43 IS - 2 N2 - BACKGROUND: The status of dengue genotypes involved in the recent epidemic out-breaks in Pakistan is not well defined. OBJECTIVES: We sought to analyze the predominant genotype responsible for the most severe and largest out-break of dengue hemorrhagic fever (DHF) that hit Karachi in 2006. STUDY DESIGN: Retrospective analysis of stored serum samples for dengue virus genotype by multiplex RT-PCR, anti-dengue IgM, IgG and review of clinical charts of patients admitted to Aga Khan University Hospital. RESULTS: Viral RNA detection of 250 patients revealed positive results in 185 (74.0%) samples. DEN-2 was predominant genotype (n=104, 56.2%) Dengue specific antibodies were detected in 139 samples of which 81 were classified as primary cases. DEN-2 accounted for these. Within secondary cases, 63.2% were due to DEN-2 (total 57), the rest were positive for DEN-3. DHF (p=0.064) and abdominal pain (p=0.059) were more frequently associated with DEN-2 as compared to DEN-3. None of the samples were positive for DEN-1 or DEN-4. CONCLUSION: Co-circulation of DEN-2 and DEN-3 was responsible for the 2006 out-break in Karachi. Primary and secondary cases were seen in both groups. Cases with DHF showed marginal association with DEN-2. Introduction of a new serotype (DEN-3) and or a genotypic shift of endemic serotype (DEN-2) are the probable factors for the recent out-break of DHF in this region. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/18639489/Co_circulations_of_two_genotypes_of_dengue_virus_in_2006_out_break_of_dengue_hemorrhagic_fever_in_Karachi_Pakistan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(08)00214-X DB - PRIME DP - Unbound Medicine ER -