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A compensatory increase in trehalose synthesis in response to desiccation stress in Saccharomyces cerevisiae cells lacking the heat shock protein Hsp12p.
Can J Microbiol. 2008 Jul; 54(7):559-68.CJ

Abstract

The effect of HSP12 deletion on the response of yeast to desiccation was investigated. The Deltahsp12 strain was found to be more desiccation tolerant than the wild-type strain. Furthermore, the increased intracellular trehalose levels in the Deltahsp12 strain suggested that this strain compensated for the lack of Hsp12p synthesis by increasing trehalose synthesis, which facilitated increased desiccation tolerance. Results obtained from flow cytometry using the membrane exclusion dye propidium iodide suggested that Hsp12p helped maintain plasma membrane integrity during desiccation. Analysis of the oxidative loads experienced by the wild-type and Deltahsp12 strains showed that during mid-exponential phase, the increased trehalose levels present in the Deltahsp12 cells resulted in increased protection of these cells against reactive oxygen species compared with wild-type cells. During stationary phase, lower levels of reactive oxygen species reduction by reduced glutathione was enhanced in the wild-type strain, which displayed lower intracellular trehalose concentrations. Comparison of the tolerance of the wild-type and Deltahsp12 strains with applied oxidative stress showed that the Deltahsp12 strain was more tolerant to exogenously applied H2O2, which we attributed to the higher intracellular trehalose concentration. Flow cytometry demonstrated that Hsp12p played a role in maintaining plasma membrane integrity during applied oxidative stress.

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Authors+Show Affiliations

Shamrock VJ
Department of Molecular and Cell Biology, University of Cape Town, Private Bag 7725, Rondebosch, Cape 7700, South Africa.
Lindsey GG
No affiliation info available

MeSH

Cell MembraneDesiccationHeat-Shock ProteinsOxidative StressReactive Oxygen SpeciesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrehalose

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18641702

Citation

Shamrock, Vanessa J., and George G. Lindsey. "A Compensatory Increase in Trehalose Synthesis in Response to Desiccation Stress in Saccharomyces Cerevisiae Cells Lacking the Heat Shock Protein Hsp12p." Canadian Journal of Microbiology, vol. 54, no. 7, 2008, pp. 559-68.
Shamrock VJ, Lindsey GG. A compensatory increase in trehalose synthesis in response to desiccation stress in Saccharomyces cerevisiae cells lacking the heat shock protein Hsp12p. Can J Microbiol. 2008;54(7):559-68.
Shamrock, V. J., & Lindsey, G. G. (2008). A compensatory increase in trehalose synthesis in response to desiccation stress in Saccharomyces cerevisiae cells lacking the heat shock protein Hsp12p. Canadian Journal of Microbiology, 54(7), 559-68. https://doi.org/10.1139/w08-044
Shamrock VJ, Lindsey GG. A Compensatory Increase in Trehalose Synthesis in Response to Desiccation Stress in Saccharomyces Cerevisiae Cells Lacking the Heat Shock Protein Hsp12p. Can J Microbiol. 2008;54(7):559-68. PubMed PMID: 18641702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A compensatory increase in trehalose synthesis in response to desiccation stress in Saccharomyces cerevisiae cells lacking the heat shock protein Hsp12p. AU - Shamrock,Vanessa J, AU - Lindsey,George G, PY - 2008/7/22/pubmed PY - 2008/10/8/medline PY - 2008/7/22/entrez SP - 559 EP - 68 JF - Canadian journal of microbiology JO - Can J Microbiol VL - 54 IS - 7 N2 - The effect of HSP12 deletion on the response of yeast to desiccation was investigated. The Deltahsp12 strain was found to be more desiccation tolerant than the wild-type strain. Furthermore, the increased intracellular trehalose levels in the Deltahsp12 strain suggested that this strain compensated for the lack of Hsp12p synthesis by increasing trehalose synthesis, which facilitated increased desiccation tolerance. Results obtained from flow cytometry using the membrane exclusion dye propidium iodide suggested that Hsp12p helped maintain plasma membrane integrity during desiccation. Analysis of the oxidative loads experienced by the wild-type and Deltahsp12 strains showed that during mid-exponential phase, the increased trehalose levels present in the Deltahsp12 cells resulted in increased protection of these cells against reactive oxygen species compared with wild-type cells. During stationary phase, lower levels of reactive oxygen species reduction by reduced glutathione was enhanced in the wild-type strain, which displayed lower intracellular trehalose concentrations. Comparison of the tolerance of the wild-type and Deltahsp12 strains with applied oxidative stress showed that the Deltahsp12 strain was more tolerant to exogenously applied H2O2, which we attributed to the higher intracellular trehalose concentration. Flow cytometry demonstrated that Hsp12p played a role in maintaining plasma membrane integrity during applied oxidative stress. SN - 0008-4166 UR - https://www.unboundmedicine.com/medline/citation/18641702/A_compensatory_increase_in_trehalose_synthesis_in_response_to_desiccation_stress_in_Saccharomyces_cerevisiae_cells_lacking_the_heat_shock_protein_Hsp12p_ L2 - https://cdnsciencepub.com/doi/10.1139/w08-044?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -