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Inhibition of nasopharyngeal carcinoma growth by RTA-expressing baculovirus vectors containing oriP.
J Gene Med. 2008 Oct; 10(10):1124-33.JG

Abstract

BACKGROUND

Nasopharyngeal carcinoma (NPC) is closely associated with latent Epstein-Barr virus (EBV) infection. Activation of latent EBV into lytic replication by introducing viral lytic gene BRLF1 (RTA) has been shown to be a potential approach to suppress the growth of EBV-associated NPC tumor.

METHODS

The baculovirus vectors with RTA expression cassette (BV-R), RTA and the EBV latent replication origin (OriP, BV-RO), or RTA, OriP and EBNA-1 gene (BV-ROE-CMV), were constructed and examined for their ability to mediate RTA expression, initiate lytic replication and induce cell death in EBV-positive cell lines Hone1-EBV, HK1-EBV and C666-1 in vitro. Their effect to inhibit the growth of EBV-positive NPC tumors was also evaluated in nude mice.

RESULTS

The baculovirus vectors BV-RO and BV-ROE-CMV mediated efficient expression of RTA in EBV-positive NPC cells. Lytic EBV replication and cell death were observed in these infected cells. Both vectors also significantly inhibited the growth of EBV-positive NPC tumor in nude mice. EBV early lytic gene expression and tumor cell death were observed in these treated tumors.

CONCLUSIONS

The presence of OriP improved the performance of the RTA-expressing baculovirus vectors to induce EBV lytic replication and cell death in vitro, and suppress the growth of EBV positive NPC tumors in vivo. By confining RTA and EBNA-1 expression to EBV-positive cells, BV-RO is expected to be a better candidate in application than BV-ROE-CMV in the long term.

Authors+Show Affiliations

Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18642396

Citation

Wang, Leyao, et al. "Inhibition of Nasopharyngeal Carcinoma Growth By RTA-expressing Baculovirus Vectors Containing OriP." The Journal of Gene Medicine, vol. 10, no. 10, 2008, pp. 1124-33.
Wang L, Shan L, Lo KW, et al. Inhibition of nasopharyngeal carcinoma growth by RTA-expressing baculovirus vectors containing oriP. J Gene Med. 2008;10(10):1124-33.
Wang, L., Shan, L., Lo, K. W., Yin, J., Zhang, Y., Sun, R., & Zhong, J. (2008). Inhibition of nasopharyngeal carcinoma growth by RTA-expressing baculovirus vectors containing oriP. The Journal of Gene Medicine, 10(10), 1124-33. https://doi.org/10.1002/jgm.1237
Wang L, et al. Inhibition of Nasopharyngeal Carcinoma Growth By RTA-expressing Baculovirus Vectors Containing OriP. J Gene Med. 2008;10(10):1124-33. PubMed PMID: 18642396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of nasopharyngeal carcinoma growth by RTA-expressing baculovirus vectors containing oriP. AU - Wang,Leyao, AU - Shan,Liang, AU - Lo,Kwok Wai, AU - Yin,Juan, AU - Zhang,Yuanyuan, AU - Sun,Ren, AU - Zhong,Jiang, PY - 2008/7/22/pubmed PY - 2008/12/17/medline PY - 2008/7/22/entrez SP - 1124 EP - 33 JF - The journal of gene medicine JO - J Gene Med VL - 10 IS - 10 N2 - BACKGROUND: Nasopharyngeal carcinoma (NPC) is closely associated with latent Epstein-Barr virus (EBV) infection. Activation of latent EBV into lytic replication by introducing viral lytic gene BRLF1 (RTA) has been shown to be a potential approach to suppress the growth of EBV-associated NPC tumor. METHODS: The baculovirus vectors with RTA expression cassette (BV-R), RTA and the EBV latent replication origin (OriP, BV-RO), or RTA, OriP and EBNA-1 gene (BV-ROE-CMV), were constructed and examined for their ability to mediate RTA expression, initiate lytic replication and induce cell death in EBV-positive cell lines Hone1-EBV, HK1-EBV and C666-1 in vitro. Their effect to inhibit the growth of EBV-positive NPC tumors was also evaluated in nude mice. RESULTS: The baculovirus vectors BV-RO and BV-ROE-CMV mediated efficient expression of RTA in EBV-positive NPC cells. Lytic EBV replication and cell death were observed in these infected cells. Both vectors also significantly inhibited the growth of EBV-positive NPC tumor in nude mice. EBV early lytic gene expression and tumor cell death were observed in these treated tumors. CONCLUSIONS: The presence of OriP improved the performance of the RTA-expressing baculovirus vectors to induce EBV lytic replication and cell death in vitro, and suppress the growth of EBV positive NPC tumors in vivo. By confining RTA and EBNA-1 expression to EBV-positive cells, BV-RO is expected to be a better candidate in application than BV-ROE-CMV in the long term. SN - 1521-2254 UR - https://www.unboundmedicine.com/medline/citation/18642396/Inhibition_of_nasopharyngeal_carcinoma_growth_by_RTA_expressing_baculovirus_vectors_containing_oriP_ L2 - https://doi.org/10.1002/jgm.1237 DB - PRIME DP - Unbound Medicine ER -