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Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil.
Plant Biotechnol J. 2008 Oct; 6(8):819-31.PB

Abstract

SUMMARY

A central goal of green chemistry is to produce industrially useful fatty acids in oilseed crops. Although genes encoding suitable fatty acid-modifying enzymes are available from many wild species, progress has been limited because the expression of these genes in transgenic plants produces low yields of the desired products. For example, Ricinus communis fatty acid hydroxylase 12 (FAH12) produces a maximum of only 17% hydroxy fatty acids (HFAs) when expressed in Arabidopsis. cDNA clones encoding R. communis enzymes for additional steps in the seed oil biosynthetic pathway were identified. Expression of these cDNAs in FAH12 transgenic plants revealed that the R. communis type-2 acyl-coenzyme A:diacylglycerol acyltransferase (RcDGAT2) could increase HFAs from 17% to nearly 30%. Detailed comparisons of seed neutral lipids from the single- and double-transgenic lines indicated that RcDGAT2 substantially modified the triacylglycerol (TAG) pool, with significant increases in most of the major TAG species observed in native castor bean oil. These data suggest that RcDGAT2 prefers acyl-coenzyme A and diacylglycerol substrates containing HFAs, and biochemical analyses of RcDGAT2 expressed in yeast cells confirmed a strong preference for HFA-containing diacylglycerol substrates. Our results demonstrate that pathway engineering approaches can be used successfully to increase the yields of industrial feedstocks in plants, and that members of the DGAT2 gene family probably play a key role in this process.

Authors+Show Affiliations

Institute of Biological Chemistry, Clark Hall, Washington State University, Pullman, WA 99164-6340, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18643899

Citation

Burgal, Julie, et al. "Metabolic Engineering of Hydroxy Fatty Acid Production in Plants: RcDGAT2 Drives Dramatic Increases in Ricinoleate Levels in Seed Oil." Plant Biotechnology Journal, vol. 6, no. 8, 2008, pp. 819-31.
Burgal J, Shockey J, Lu C, et al. Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil. Plant Biotechnol J. 2008;6(8):819-31.
Burgal, J., Shockey, J., Lu, C., Dyer, J., Larson, T., Graham, I., & Browse, J. (2008). Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil. Plant Biotechnology Journal, 6(8), 819-31. https://doi.org/10.1111/j.1467-7652.2008.00361.x
Burgal J, et al. Metabolic Engineering of Hydroxy Fatty Acid Production in Plants: RcDGAT2 Drives Dramatic Increases in Ricinoleate Levels in Seed Oil. Plant Biotechnol J. 2008;6(8):819-31. PubMed PMID: 18643899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil. AU - Burgal,Julie, AU - Shockey,Jay, AU - Lu,Chaofu, AU - Dyer,John, AU - Larson,Tony, AU - Graham,Ian, AU - Browse,John, Y1 - 2008/07/14/ PY - 2008/7/23/pubmed PY - 2008/11/6/medline PY - 2008/7/23/entrez SP - 819 EP - 31 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 6 IS - 8 N2 - SUMMARY: A central goal of green chemistry is to produce industrially useful fatty acids in oilseed crops. Although genes encoding suitable fatty acid-modifying enzymes are available from many wild species, progress has been limited because the expression of these genes in transgenic plants produces low yields of the desired products. For example, Ricinus communis fatty acid hydroxylase 12 (FAH12) produces a maximum of only 17% hydroxy fatty acids (HFAs) when expressed in Arabidopsis. cDNA clones encoding R. communis enzymes for additional steps in the seed oil biosynthetic pathway were identified. Expression of these cDNAs in FAH12 transgenic plants revealed that the R. communis type-2 acyl-coenzyme A:diacylglycerol acyltransferase (RcDGAT2) could increase HFAs from 17% to nearly 30%. Detailed comparisons of seed neutral lipids from the single- and double-transgenic lines indicated that RcDGAT2 substantially modified the triacylglycerol (TAG) pool, with significant increases in most of the major TAG species observed in native castor bean oil. These data suggest that RcDGAT2 prefers acyl-coenzyme A and diacylglycerol substrates containing HFAs, and biochemical analyses of RcDGAT2 expressed in yeast cells confirmed a strong preference for HFA-containing diacylglycerol substrates. Our results demonstrate that pathway engineering approaches can be used successfully to increase the yields of industrial feedstocks in plants, and that members of the DGAT2 gene family probably play a key role in this process. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/18643899/Metabolic_engineering_of_hydroxy_fatty_acid_production_in_plants:_RcDGAT2_drives_dramatic_increases_in_ricinoleate_levels_in_seed_oil_ L2 - https://doi.org/10.1111/j.1467-7652.2008.00361.x DB - PRIME DP - Unbound Medicine ER -