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Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7.
Biosens Bioelectron. 2008 Dec 01; 24(4):558-65.BB

Abstract

This work demonstrated the feasibility of detecting 250zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.

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Publisher Full Text

Authors+Show Affiliations

Liu YJ
Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
Yao DJ
No affiliation info available
Chang HY
No affiliation info available
Liu CM
No affiliation info available
Chen C
No affiliation info available

MeSH

Biosensing TechniquesDNA, BacterialEquipment DesignEquipment Failure AnalysisEscherichia coli O157MagneticsQuantum DotsReproducibility of ResultsSensitivity and SpecificitySpectrometry, FluorescenceStaining and Labeling

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18644710

Citation

Liu, Yi-Ju, et al. "Magnetic Bead-based DNA Detection With Multi-layers Quantum Dots Labeling for Rapid Detection of Escherichia Coli O157:H7." Biosensors & Bioelectronics, vol. 24, no. 4, 2008, pp. 558-65.
Liu YJ, Yao DJ, Chang HY, et al. Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7. Biosens Bioelectron. 2008;24(4):558-65.
Liu, Y. J., Yao, D. J., Chang, H. Y., Liu, C. M., & Chen, C. (2008). Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7. Biosensors & Bioelectronics, 24(4), 558-65. https://doi.org/10.1016/j.bios.2008.06.019
Liu YJ, et al. Magnetic Bead-based DNA Detection With Multi-layers Quantum Dots Labeling for Rapid Detection of Escherichia Coli O157:H7. Biosens Bioelectron. 2008 Dec 1;24(4):558-65. PubMed PMID: 18644710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7. AU - Liu,Yi-Ju, AU - Yao,Da-Jeng, AU - Chang,Hwan-You, AU - Liu,Chien-Ming, AU - Chen,Chih, Y1 - 2008/06/20/ PY - 2008/03/16/received PY - 2008/05/05/revised PY - 2008/06/04/accepted PY - 2008/7/23/pubmed PY - 2009/2/13/medline PY - 2008/7/23/entrez SP - 558 EP - 65 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 4 N2 - This work demonstrated the feasibility of detecting 250zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18644710/Magnetic_bead_based_DNA_detection_with_multi_layers_quantum_dots_labeling_for_rapid_detection_of_Escherichia_coli_O157:H7_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00262-5 DB - PRIME DP - Unbound Medicine ER -