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Real-time protein biosensor arrays based on surface plasmon resonance differential phase imaging.
Biosens Bioelectron. 2008 Dec 01; 24(4):606-12.BB

Abstract

This paper reports the application of differential phase surface plasmon resonance (SPR) imaging in two-dimensional (2D) protein biosensor arrays. Our phase imaging approach offers a distinct advantage over the conventional angular SPR technique in terms of utilization efficiency of optical sensor elements in the imaging device. In the angular approach, each biosensor site in the biosensor array requires a linear array of optical detector elements to locate the SPR angular dip. The maximum biosensor density that a two-dimensional imaging device can offer is a one-dimensional SPR biosensor array. On the other hand, the phase-sensitive SPR approach captures data in the time domain instead of the spatial domain. It is possible that each pixel in the captured interferogram represents one sensor site, thus offering high-density two-dimensional biosensor arrays. In addition, our differential phase approach improves detection resolution through removing common-mode disturbances. Experimental results demonstrate a system resolution of 8.8 x 10(-7)RIU (refractive index unit). Real-time monitoring of bovine serum albumin (BSA)/anti-BSA binding interactions at various concentration levels was achieved using a biosensor array. The detection limit was 0.77 microg/ml. The reported two-dimensional SPR biosensor array offers a real-time and non-labeling detection tool for high-throughput protein array analysis. It may find promising applications in protein therapeutics, drug screening and clinical diagnostics.

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Authors+Show Affiliations

Wong CL
Department of Electronic Engineering, The Chinese University of Hong Kong, Hong Kong, Hong Kong, China.
Ho HP
No affiliation info available
Suen YK
No affiliation info available
Kong SK
No affiliation info available
Chen QL
No affiliation info available
Yuan W
No affiliation info available
Wu SY
No affiliation info available

MeSH

Biosensing TechniquesComputer SystemsEquipment DesignEquipment Failure AnalysisProtein Array AnalysisReproducibility of ResultsSensitivity and SpecificitySerum AlbuminSurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18644712

Citation

Wong, C L., et al. "Real-time Protein Biosensor Arrays Based On Surface Plasmon Resonance Differential Phase Imaging." Biosensors & Bioelectronics, vol. 24, no. 4, 2008, pp. 606-12.
Wong CL, Ho HP, Suen YK, et al. Real-time protein biosensor arrays based on surface plasmon resonance differential phase imaging. Biosens Bioelectron. 2008;24(4):606-12.
Wong, C. L., Ho, H. P., Suen, Y. K., Kong, S. K., Chen, Q. L., Yuan, W., & Wu, S. Y. (2008). Real-time protein biosensor arrays based on surface plasmon resonance differential phase imaging. Biosensors & Bioelectronics, 24(4), 606-12. https://doi.org/10.1016/j.bios.2008.06.013
Wong CL, et al. Real-time Protein Biosensor Arrays Based On Surface Plasmon Resonance Differential Phase Imaging. Biosens Bioelectron. 2008 Dec 1;24(4):606-12. PubMed PMID: 18644712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time protein biosensor arrays based on surface plasmon resonance differential phase imaging. AU - Wong,C L, AU - Ho,H P, AU - Suen,Y K, AU - Kong,S K, AU - Chen,Q L, AU - Yuan,W, AU - Wu,S Y, Y1 - 2008/06/17/ PY - 2008/02/29/received PY - 2008/04/18/revised PY - 2008/06/04/accepted PY - 2008/7/23/pubmed PY - 2009/2/13/medline PY - 2008/7/23/entrez SP - 606 EP - 12 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 4 N2 - This paper reports the application of differential phase surface plasmon resonance (SPR) imaging in two-dimensional (2D) protein biosensor arrays. Our phase imaging approach offers a distinct advantage over the conventional angular SPR technique in terms of utilization efficiency of optical sensor elements in the imaging device. In the angular approach, each biosensor site in the biosensor array requires a linear array of optical detector elements to locate the SPR angular dip. The maximum biosensor density that a two-dimensional imaging device can offer is a one-dimensional SPR biosensor array. On the other hand, the phase-sensitive SPR approach captures data in the time domain instead of the spatial domain. It is possible that each pixel in the captured interferogram represents one sensor site, thus offering high-density two-dimensional biosensor arrays. In addition, our differential phase approach improves detection resolution through removing common-mode disturbances. Experimental results demonstrate a system resolution of 8.8 x 10(-7)RIU (refractive index unit). Real-time monitoring of bovine serum albumin (BSA)/anti-BSA binding interactions at various concentration levels was achieved using a biosensor array. The detection limit was 0.77 microg/ml. The reported two-dimensional SPR biosensor array offers a real-time and non-labeling detection tool for high-throughput protein array analysis. It may find promising applications in protein therapeutics, drug screening and clinical diagnostics. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18644712/Real_time_protein_biosensor_arrays_based_on_surface_plasmon_resonance_differential_phase_imaging_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00254-6 DB - PRIME DP - Unbound Medicine ER -