TY - JOUR T1 - Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting. AU - Hettick,Justin M, AU - Green,Brett J, AU - Buskirk,Amanda D, AU - Kashon,Michael L, AU - Slaven,James E, AU - Janotka,Erika, AU - Blachere,Francoise M, AU - Schmechel,Detlef, AU - Beezhold,Donald H, PY - 2008/7/23/pubmed PY - 2008/9/13/medline PY - 2008/7/23/entrez SP - 2555 EP - 60 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 16 N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral 'fingerprints' for twelve Penicillium species. Prior to MALDI-TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one-minute bead-beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5000-20 000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13 900. Discriminant analysis using the MALDI-TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18646251/Discrimination_of_Penicillium_isolates_by_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_fingerprinting_ L2 - https://doi.org/10.1002/rcm.3649 DB - PRIME DP - Unbound Medicine ER -