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Effect of a carbon source on polyphosphate accumulation in Saccharomyces cerevisiae.
FEMS Yeast Res. 2008 Sep; 8(6):877-82.FY

Abstract

The cells of Saccharomyces cerevisiae accumulate inorganic polyphosphate (polyP) when reinoculated on a phosphate-containing medium after phosphorus starvation. Total polyP accumulation was similar at cultivation on both glucose and ethanol. Five separate fractions of polyP: acid-soluble fraction polyP1, salt-soluble fraction polyP2, weakly alkali-soluble fraction polyP3, alkali-soluble fraction polyP4, and polyP5, have been obtained from the cells grown on glucose and ethanol under phosphate overplus. The dynamics of polyP fractions depend on a carbon source. The accumulation rates for fractions polyP2 and polyP4 were independent of the carbon source. The accumulation rates of polyP1 and polyP3 were higher on glucose, while fraction polyP5 accumulated faster on ethanol. As to the maximal polyP levels, they were independent of the carbon source for fractions polyP2, polyP3, and polyP4. The maximal level of fraction polyP1 was higher on glucose than on ethanol, but the level of fraction polyP5 was higher on ethanol. It was assumed that accumulation of separate polyP fractions has a metabolic interrelation with different energy-providing pathways. The polyphosphate nature of fraction polyP5 was demonstrated for the first time by (31)P nuclear magnetic resonance spectroscopy, enzymatic assay, and electrophoresis.

Authors+Show Affiliations

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow, Russia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18647178

Citation

Vagabov, Vladimir M., et al. "Effect of a Carbon Source On Polyphosphate Accumulation in Saccharomyces Cerevisiae." FEMS Yeast Research, vol. 8, no. 6, 2008, pp. 877-82.
Vagabov VM, Trilisenko LV, Kulakovskaya TV, et al. Effect of a carbon source on polyphosphate accumulation in Saccharomyces cerevisiae. FEMS Yeast Res. 2008;8(6):877-82.
Vagabov, V. M., Trilisenko, L. V., Kulakovskaya, T. V., & Kulaev, I. S. (2008). Effect of a carbon source on polyphosphate accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 8(6), 877-82. https://doi.org/10.1111/j.1567-1364.2008.00420.x
Vagabov VM, et al. Effect of a Carbon Source On Polyphosphate Accumulation in Saccharomyces Cerevisiae. FEMS Yeast Res. 2008;8(6):877-82. PubMed PMID: 18647178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of a carbon source on polyphosphate accumulation in Saccharomyces cerevisiae. AU - Vagabov,Vladimir M, AU - Trilisenko,Ludmila V, AU - Kulakovskaya,Tatiana V, AU - Kulaev,Igor S, Y1 - 2008/07/17/ PY - 2008/7/24/pubmed PY - 2008/12/17/medline PY - 2008/7/24/entrez SP - 877 EP - 82 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 6 N2 - The cells of Saccharomyces cerevisiae accumulate inorganic polyphosphate (polyP) when reinoculated on a phosphate-containing medium after phosphorus starvation. Total polyP accumulation was similar at cultivation on both glucose and ethanol. Five separate fractions of polyP: acid-soluble fraction polyP1, salt-soluble fraction polyP2, weakly alkali-soluble fraction polyP3, alkali-soluble fraction polyP4, and polyP5, have been obtained from the cells grown on glucose and ethanol under phosphate overplus. The dynamics of polyP fractions depend on a carbon source. The accumulation rates for fractions polyP2 and polyP4 were independent of the carbon source. The accumulation rates of polyP1 and polyP3 were higher on glucose, while fraction polyP5 accumulated faster on ethanol. As to the maximal polyP levels, they were independent of the carbon source for fractions polyP2, polyP3, and polyP4. The maximal level of fraction polyP1 was higher on glucose than on ethanol, but the level of fraction polyP5 was higher on ethanol. It was assumed that accumulation of separate polyP fractions has a metabolic interrelation with different energy-providing pathways. The polyphosphate nature of fraction polyP5 was demonstrated for the first time by (31)P nuclear magnetic resonance spectroscopy, enzymatic assay, and electrophoresis. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/18647178/Effect_of_a_carbon_source_on_polyphosphate_accumulation_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2008.00420.x DB - PRIME DP - Unbound Medicine ER -