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Sodium nitroprusside induces mild oxidative stress in Saccharomyces cerevisiae.
Redox Rep. 2008; 13(4):144-52.RR

Abstract

Nitric oxide is known to be a messenger in animals and plants. It may act either as a pro-oxidant or antioxidant. In the present work, the yeast Saccharomyces cerevisiae was treated under aerobic conditions with the nitric oxide donor, sodium nitroprusside (SNP), at concentrations of 1, 5 and 10 mM. The activities of antioxidant enzymes as well as concentrations of protein carbonyls and cellular thiols were measured. Yeast incubation with SNP increased the activities of catalase and superoxide dismutase. Cycloheximide, an inhibitor of translation, blocked SNP-induced catalase activation, but not SOD activation. Incubation with SNP increased the activity of peroxisomal catalase, whereas cytosolic catalase was not affected. SNP treatment inactivated aconitase in a dose-dependent manner. Surprisingly, in cells incubated with 1 mM SNP, the levels of low-molecular weight thiols were significantly higher, whereas the concentrations of protein carbonyl groups were lower than those in untreated cells. The incubation of yeast cells either with decomposed SNP or with SNP under anaerobic conditions did not result in SOD and catalase activation. It is suggested, that under aerobic conditions, the SNP effects are connected with induction of mild oxidative/nitrosative stress.

Authors+Show Affiliations

Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18647484

Citation

Lushchak, Oleh V., and Volodymyr I. Lushchak. "Sodium Nitroprusside Induces Mild Oxidative Stress in Saccharomyces Cerevisiae." Redox Report : Communications in Free Radical Research, vol. 13, no. 4, 2008, pp. 144-52.
Lushchak OV, Lushchak VI. Sodium nitroprusside induces mild oxidative stress in Saccharomyces cerevisiae. Redox Rep. 2008;13(4):144-52.
Lushchak, O. V., & Lushchak, V. I. (2008). Sodium nitroprusside induces mild oxidative stress in Saccharomyces cerevisiae. Redox Report : Communications in Free Radical Research, 13(4), 144-52. https://doi.org/10.1179/135100008X308885
Lushchak OV, Lushchak VI. Sodium Nitroprusside Induces Mild Oxidative Stress in Saccharomyces Cerevisiae. Redox Rep. 2008;13(4):144-52. PubMed PMID: 18647484.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sodium nitroprusside induces mild oxidative stress in Saccharomyces cerevisiae. AU - Lushchak,Oleh V, AU - Lushchak,Volodymyr I, PY - 2008/7/24/pubmed PY - 2008/12/17/medline PY - 2008/7/24/entrez SP - 144 EP - 52 JF - Redox report : communications in free radical research JO - Redox Rep VL - 13 IS - 4 N2 - Nitric oxide is known to be a messenger in animals and plants. It may act either as a pro-oxidant or antioxidant. In the present work, the yeast Saccharomyces cerevisiae was treated under aerobic conditions with the nitric oxide donor, sodium nitroprusside (SNP), at concentrations of 1, 5 and 10 mM. The activities of antioxidant enzymes as well as concentrations of protein carbonyls and cellular thiols were measured. Yeast incubation with SNP increased the activities of catalase and superoxide dismutase. Cycloheximide, an inhibitor of translation, blocked SNP-induced catalase activation, but not SOD activation. Incubation with SNP increased the activity of peroxisomal catalase, whereas cytosolic catalase was not affected. SNP treatment inactivated aconitase in a dose-dependent manner. Surprisingly, in cells incubated with 1 mM SNP, the levels of low-molecular weight thiols were significantly higher, whereas the concentrations of protein carbonyl groups were lower than those in untreated cells. The incubation of yeast cells either with decomposed SNP or with SNP under anaerobic conditions did not result in SOD and catalase activation. It is suggested, that under aerobic conditions, the SNP effects are connected with induction of mild oxidative/nitrosative stress. SN - 1743-2928 UR - https://www.unboundmedicine.com/medline/citation/18647484/Sodium_nitroprusside_induces_mild_oxidative_stress_in_Saccharomyces_cerevisiae_ L2 - https://www.tandfonline.com/doi/full/10.1179/135100008X308885 DB - PRIME DP - Unbound Medicine ER -