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Long-term genotoxic effect of monocrotophos in different tissues of freshwater fish Channa punctatus (Bloch) using alkaline single cell gel electrophoresis.
Sci Total Environ. 2008 Nov 01; 405(1-3):345-50.ST

Abstract

Monocrotophos, commonly known as azodrin, is one of the organophosphate pesticides extensively used in agricultural practices throughout the world. However, little information is available on its long-term genotoxic effects in different tissues of fish using genotoxicity biomarkers. The objective of the present study was to detect DNA damage, induced by monocrotophos in freshwater teleost fish Channa punctatus using the comet assay. The LC(50)-96 h value of technical-grade monocrotophos was estimated for the fish species in a semi-static system. On the basis of the LC(50) value, the sublethal and nonlethal concentrations were determined. The DNA damage was measured in the gill, kidney and lymphocytes as the percentage of DNA in comet tails of fish exposed to different sublethal and nonlethal concentrations of monocrotophos. In general, significant effects (P<0.01) from both concentration and time of exposure were observed in exposed fish. It was found that the tissues at all concentrations exhibited the highest DNA damage on day 4, after which there was a decline in percentage tail DNA. The comparison of DNA damage among tissues at different concentrations indicated that the gill cells were more sensitive to the pesticide than the kidney cells and lymphocytes. This study also explored the utility of the comet assay for in vivo laboratory studies using fish for screening the genotoxic potential of various agents.

Authors+Show Affiliations

Molecular & Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow, India.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

18649923

Citation

Ali, Daoud, and Sudhir Kumar. "Long-term Genotoxic Effect of Monocrotophos in Different Tissues of Freshwater Fish Channa Punctatus (Bloch) Using Alkaline Single Cell Gel Electrophoresis." The Science of the Total Environment, vol. 405, no. 1-3, 2008, pp. 345-50.
Ali D, Kumar S. Long-term genotoxic effect of monocrotophos in different tissues of freshwater fish Channa punctatus (Bloch) using alkaline single cell gel electrophoresis. Sci Total Environ. 2008;405(1-3):345-50.
Ali, D., & Kumar, S. (2008). Long-term genotoxic effect of monocrotophos in different tissues of freshwater fish Channa punctatus (Bloch) using alkaline single cell gel electrophoresis. The Science of the Total Environment, 405(1-3), 345-50. https://doi.org/10.1016/j.scitotenv.2008.05.037
Ali D, Kumar S. Long-term Genotoxic Effect of Monocrotophos in Different Tissues of Freshwater Fish Channa Punctatus (Bloch) Using Alkaline Single Cell Gel Electrophoresis. Sci Total Environ. 2008 Nov 1;405(1-3):345-50. PubMed PMID: 18649923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term genotoxic effect of monocrotophos in different tissues of freshwater fish Channa punctatus (Bloch) using alkaline single cell gel electrophoresis. AU - Ali,Daoud, AU - Kumar,Sudhir, Y1 - 2008/07/22/ PY - 2008/03/20/received PY - 2008/05/22/revised PY - 2008/05/27/accepted PY - 2008/7/25/pubmed PY - 2009/1/16/medline PY - 2008/7/25/entrez SP - 345 EP - 50 JF - The Science of the total environment JO - Sci Total Environ VL - 405 IS - 1-3 N2 - Monocrotophos, commonly known as azodrin, is one of the organophosphate pesticides extensively used in agricultural practices throughout the world. However, little information is available on its long-term genotoxic effects in different tissues of fish using genotoxicity biomarkers. The objective of the present study was to detect DNA damage, induced by monocrotophos in freshwater teleost fish Channa punctatus using the comet assay. The LC(50)-96 h value of technical-grade monocrotophos was estimated for the fish species in a semi-static system. On the basis of the LC(50) value, the sublethal and nonlethal concentrations were determined. The DNA damage was measured in the gill, kidney and lymphocytes as the percentage of DNA in comet tails of fish exposed to different sublethal and nonlethal concentrations of monocrotophos. In general, significant effects (P<0.01) from both concentration and time of exposure were observed in exposed fish. It was found that the tissues at all concentrations exhibited the highest DNA damage on day 4, after which there was a decline in percentage tail DNA. The comparison of DNA damage among tissues at different concentrations indicated that the gill cells were more sensitive to the pesticide than the kidney cells and lymphocytes. This study also explored the utility of the comet assay for in vivo laboratory studies using fish for screening the genotoxic potential of various agents. SN - 0048-9697 UR - https://www.unboundmedicine.com/medline/citation/18649923/Long_term_genotoxic_effect_of_monocrotophos_in_different_tissues_of_freshwater_fish_Channa_punctatus__Bloch__using_alkaline_single_cell_gel_electrophoresis_ DB - PRIME DP - Unbound Medicine ER -