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Establishment of a liquid chromatographic/mass spectrometry method for quantification of tetrandrine in rat plasma and its application to pharmacokinetic study.
J Pharm Biomed Anal. 2008 Nov 04; 48(3):974-9.JP

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometric method (LC/MS/MS) for the determination of tetrandrine in rat plasma has been developed, fully validated and successfully applied to pharmacokinetic study in Sprague-Dawley (SD) rats after a single oral administration. Sample preparation involves a liquid-liquid extraction with n-hexane-dichlormethane (65:35, containing 1% 2-propanol isopropyl alcohol, v/v). Tetrandrine and brodimoprim (internal standard) were well separated by LC with a Dikma C(18) column using acetonitrile-methanol-ammonium formate aqueous solution (20mM) containing 0.3% formic acid (20:30:50, v/v/v) as mobile phase. Detection was performed on a triple quadrupole mass spectrometer in multiple reaction monitoring mode. The ionization was optimized using ESI(+) and selectivity was achieved using MS/MS analysis, m/z 623.0-->381.0 and m/z 339.0-->281.0 for tetrandrine and I.S., respectively. The present method exhibited good linearity over the concentration range of 5-2,000 ng/mL for tetrandrine in rat plasma with a lower limit of quantification of 5 ng/mL. The intra- and inter-day precision were 2.0-9.2% and 4.5-9.4%, and the intra- and inter-day accuracy ranged from -7.6 to 10.3% and -6.0 to 5.3%, respectively. No endogenous compounds were found to interfere with the analysis, and tetrandrine was stable during the whole assay period. The method was successfully applied to a pharmacokinetic study after an intragastric administration (i.g.) of tetrandrine to SD rats with a single dose of 50mg/kg. The results confirm that the assay is suitable for the pharmacokinetic study of tetrandrine.

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Authors+Show Affiliations

Song N
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Zhang S
No affiliation info available
Li Q
No affiliation info available
Liu C
No affiliation info available

MeSH

AnimalsArea Under CurveBenzylisoquinolinesCalibrationChromatography, LiquidDrug StabilityFastingFemaleHalf-LifeImmunosuppressive AgentsMaleMass SpectrometryMolecular StructureQuality ControlRatsRats, Sprague-DawleyReference StandardsReproducibility of ResultsSensitivity and SpecificityTime FactorsTrimethoprim

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18650047

Citation

Song, Naining, et al. "Establishment of a Liquid Chromatographic/mass Spectrometry Method for Quantification of Tetrandrine in Rat Plasma and Its Application to Pharmacokinetic Study." Journal of Pharmaceutical and Biomedical Analysis, vol. 48, no. 3, 2008, pp. 974-9.
Song N, Zhang S, Li Q, et al. Establishment of a liquid chromatographic/mass spectrometry method for quantification of tetrandrine in rat plasma and its application to pharmacokinetic study. J Pharm Biomed Anal. 2008;48(3):974-9.
Song, N., Zhang, S., Li, Q., & Liu, C. (2008). Establishment of a liquid chromatographic/mass spectrometry method for quantification of tetrandrine in rat plasma and its application to pharmacokinetic study. Journal of Pharmaceutical and Biomedical Analysis, 48(3), 974-9. https://doi.org/10.1016/j.jpba.2008.06.002
Song N, et al. Establishment of a Liquid Chromatographic/mass Spectrometry Method for Quantification of Tetrandrine in Rat Plasma and Its Application to Pharmacokinetic Study. J Pharm Biomed Anal. 2008 Nov 4;48(3):974-9. PubMed PMID: 18650047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishment of a liquid chromatographic/mass spectrometry method for quantification of tetrandrine in rat plasma and its application to pharmacokinetic study. AU - Song,Naining, AU - Zhang,Shaoyu, AU - Li,Quansheng, AU - Liu,Changxiao, Y1 - 2008/06/11/ PY - 2008/03/13/received PY - 2008/06/04/revised PY - 2008/06/05/accepted PY - 2008/7/25/pubmed PY - 2009/2/27/medline PY - 2008/7/25/entrez SP - 974 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 48 IS - 3 N2 - A rapid and sensitive liquid chromatography-tandem mass spectrometric method (LC/MS/MS) for the determination of tetrandrine in rat plasma has been developed, fully validated and successfully applied to pharmacokinetic study in Sprague-Dawley (SD) rats after a single oral administration. Sample preparation involves a liquid-liquid extraction with n-hexane-dichlormethane (65:35, containing 1% 2-propanol isopropyl alcohol, v/v). Tetrandrine and brodimoprim (internal standard) were well separated by LC with a Dikma C(18) column using acetonitrile-methanol-ammonium formate aqueous solution (20mM) containing 0.3% formic acid (20:30:50, v/v/v) as mobile phase. Detection was performed on a triple quadrupole mass spectrometer in multiple reaction monitoring mode. The ionization was optimized using ESI(+) and selectivity was achieved using MS/MS analysis, m/z 623.0-->381.0 and m/z 339.0-->281.0 for tetrandrine and I.S., respectively. The present method exhibited good linearity over the concentration range of 5-2,000 ng/mL for tetrandrine in rat plasma with a lower limit of quantification of 5 ng/mL. The intra- and inter-day precision were 2.0-9.2% and 4.5-9.4%, and the intra- and inter-day accuracy ranged from -7.6 to 10.3% and -6.0 to 5.3%, respectively. No endogenous compounds were found to interfere with the analysis, and tetrandrine was stable during the whole assay period. The method was successfully applied to a pharmacokinetic study after an intragastric administration (i.g.) of tetrandrine to SD rats with a single dose of 50mg/kg. The results confirm that the assay is suitable for the pharmacokinetic study of tetrandrine. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/18650047/Establishment_of_a_liquid_chromatographic/mass_spectrometry_method_for_quantification_of_tetrandrine_in_rat_plasma_and_its_application_to_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(08)00347-6 DB - PRIME DP - Unbound Medicine ER -
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