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Extracellular matrix proteoglycan decorin-mediated myogenic satellite cell responsiveness to transforming growth factor-beta1 during cell proliferation and differentiation Decorin and transforming growth factor-beta1 in satellite cells.
Domest Anim Endocrinol. 2008 Oct; 35(3):263-73.DA

Abstract

Transforming growth factor-beta1 (TGF-beta1) is a potent inhibitor of muscle cell proliferation and differentiation. Decorin, a small proteoglycan in the extracellular matrix, binds to TGF-beta1 and modulates the activity of TGF-beta1 during muscle cell growth and development. However, its interaction with TGF-beta1 and involvement in myogenesis is not well characterized. In the present study, chicken myogenic satellite cells, myogenic precursors for muscle growth and repair, were isolated from the pectoralis major muscle and used to investigate the biological function of TGF-beta1 and decorin during myogenesis. The over-expression of decorin in satellite cells significantly increased cell proliferation, compared to the control cells. Consistent with this result, reducing decorin expression decreased cell proliferation, which suggests a decorin-mediated mechanism is involved in the regulation of myogenic satellite cell proliferation. Satellite cells over-expressing decorin were less sensitive to TGF-beta1 during proliferation, which indicates that decorin may sequester TGF-beta1 leading to increased proliferation. During satellite cell differentiation, the over-expression of decorin induced differentiation by increasing the muscle specific creatine kinase concentration. However, the addition of TGF-beta1 diminished decorin-mediated cell responsiveness to TGF-beta1 during differentiation. Taken together, these results suggest that decorin induces myogenic satellite cell proliferation and differentiation by regulating cellular responsiveness to TGF-beta1. An alternative TGF-beta1-independent pathway may be involved in the regulation of satellite cells by decorin.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18650056

Citation

Li, Xuehui, et al. "Extracellular Matrix Proteoglycan Decorin-mediated Myogenic Satellite Cell Responsiveness to Transforming Growth Factor-beta1 During Cell Proliferation and Differentiation Decorin and Transforming Growth Factor-beta1 in Satellite Cells." Domestic Animal Endocrinology, vol. 35, no. 3, 2008, pp. 263-73.
Li X, McFarland DC, Velleman SG. Extracellular matrix proteoglycan decorin-mediated myogenic satellite cell responsiveness to transforming growth factor-beta1 during cell proliferation and differentiation Decorin and transforming growth factor-beta1 in satellite cells. Domest Anim Endocrinol. 2008;35(3):263-73.
Li, X., McFarland, D. C., & Velleman, S. G. (2008). Extracellular matrix proteoglycan decorin-mediated myogenic satellite cell responsiveness to transforming growth factor-beta1 during cell proliferation and differentiation Decorin and transforming growth factor-beta1 in satellite cells. Domestic Animal Endocrinology, 35(3), 263-73. https://doi.org/10.1016/j.domaniend.2008.06.002
Li X, McFarland DC, Velleman SG. Extracellular Matrix Proteoglycan Decorin-mediated Myogenic Satellite Cell Responsiveness to Transforming Growth Factor-beta1 During Cell Proliferation and Differentiation Decorin and Transforming Growth Factor-beta1 in Satellite Cells. Domest Anim Endocrinol. 2008;35(3):263-73. PubMed PMID: 18650056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extracellular matrix proteoglycan decorin-mediated myogenic satellite cell responsiveness to transforming growth factor-beta1 during cell proliferation and differentiation Decorin and transforming growth factor-beta1 in satellite cells. AU - Li,Xuehui, AU - McFarland,Douglas C, AU - Velleman,Sandra G, Y1 - 2008/07/14/ PY - 2008/04/25/received PY - 2008/06/06/revised PY - 2008/06/07/accepted PY - 2008/7/25/pubmed PY - 2008/10/22/medline PY - 2008/7/25/entrez SP - 263 EP - 73 JF - Domestic animal endocrinology JO - Domest Anim Endocrinol VL - 35 IS - 3 N2 - Transforming growth factor-beta1 (TGF-beta1) is a potent inhibitor of muscle cell proliferation and differentiation. Decorin, a small proteoglycan in the extracellular matrix, binds to TGF-beta1 and modulates the activity of TGF-beta1 during muscle cell growth and development. However, its interaction with TGF-beta1 and involvement in myogenesis is not well characterized. In the present study, chicken myogenic satellite cells, myogenic precursors for muscle growth and repair, were isolated from the pectoralis major muscle and used to investigate the biological function of TGF-beta1 and decorin during myogenesis. The over-expression of decorin in satellite cells significantly increased cell proliferation, compared to the control cells. Consistent with this result, reducing decorin expression decreased cell proliferation, which suggests a decorin-mediated mechanism is involved in the regulation of myogenic satellite cell proliferation. Satellite cells over-expressing decorin were less sensitive to TGF-beta1 during proliferation, which indicates that decorin may sequester TGF-beta1 leading to increased proliferation. During satellite cell differentiation, the over-expression of decorin induced differentiation by increasing the muscle specific creatine kinase concentration. However, the addition of TGF-beta1 diminished decorin-mediated cell responsiveness to TGF-beta1 during differentiation. Taken together, these results suggest that decorin induces myogenic satellite cell proliferation and differentiation by regulating cellular responsiveness to TGF-beta1. An alternative TGF-beta1-independent pathway may be involved in the regulation of satellite cells by decorin. SN - 0739-7240 UR - https://www.unboundmedicine.com/medline/citation/18650056/Extracellular_matrix_proteoglycan_decorin_mediated_myogenic_satellite_cell_responsiveness_to_transforming_growth_factor_beta1_during_cell_proliferation_and_differentiation_Decorin_and_transforming_growth_factor_beta1_in_satellite_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0739-7240(08)00063-5 DB - PRIME DP - Unbound Medicine ER -