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Antioxidant potential of thyme extract: alleviation of N-nitrosodiethylamine-induced oxidative stress.
Hum Exp Toxicol 2008; 27(3):215-21HE

Abstract

Protective role of thyme extract against N-nitrosodiethylamine (NDEA)-induced oxidative stress has been evaluated in albino rats. For this, one group of rats were fed diet supplemented with thyme extract (0.5%) and served as the test group, whereas animals of the other group fed on normal diet served as the control group. The rats were fed on respective diets for a period of 2 weeks after which stress was induced to half the animals of each group by i.p. administration of NDEA at 200 mg/kg body weight. Animals were killed 48 h post stress-induction period. Feed intake and body weight decreased significantly in both test and control groups, the effect being less in test group. Increase in osmotic fragility and in-vitro lipid peroxidation (LPO) on stress induction was of lower degree in the test group. NDEA toxicity was mainly reflected in liver as evidenced by increased activities of plasma aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase. The effect was of lower degree in test group as compared with that in the control group. Increase in urea levels observed following NDEA administration was also of lower degree in test groups. Blood glutathione (GSH) levels increased more so in test group compared with control group on stress induction. The activities of superoxide dismutase (SOD), peroxidase (Px), and catalase (CAT) activities decreased significantly on stress induction in erythrocytes. LPO increased in all the tissues through varying degree, and the increase was appreciably of lower degree in test group. The activity of SOD increased significantly in both test and control group on stress induction, whereas activities of Px and CAT decreased following NDEA treatment, and the effects were of lower degree in test group. Thus, supplementation of diet with thyme extract can improve antioxygenic potential and hence help to prevent oxidative stress.

Authors+Show Affiliations

Department of Biochemistry and Chemistry, Punjab Agricultural University, Ludhiana, Punjab, India.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18650253

Citation

Rana, P, and G Soni. "Antioxidant Potential of Thyme Extract: Alleviation of N-nitrosodiethylamine-induced Oxidative Stress." Human & Experimental Toxicology, vol. 27, no. 3, 2008, pp. 215-21.
Rana P, Soni G. Antioxidant potential of thyme extract: alleviation of N-nitrosodiethylamine-induced oxidative stress. Hum Exp Toxicol. 2008;27(3):215-21.
Rana, P., & Soni, G. (2008). Antioxidant potential of thyme extract: alleviation of N-nitrosodiethylamine-induced oxidative stress. Human & Experimental Toxicology, 27(3), pp. 215-21. doi:10.1177/0960327108088970.
Rana P, Soni G. Antioxidant Potential of Thyme Extract: Alleviation of N-nitrosodiethylamine-induced Oxidative Stress. Hum Exp Toxicol. 2008;27(3):215-21. PubMed PMID: 18650253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant potential of thyme extract: alleviation of N-nitrosodiethylamine-induced oxidative stress. AU - Rana,P, AU - Soni,G, PY - 2008/7/25/pubmed PY - 2009/1/7/medline PY - 2008/7/25/entrez SP - 215 EP - 21 JF - Human & experimental toxicology JO - Hum Exp Toxicol VL - 27 IS - 3 N2 - Protective role of thyme extract against N-nitrosodiethylamine (NDEA)-induced oxidative stress has been evaluated in albino rats. For this, one group of rats were fed diet supplemented with thyme extract (0.5%) and served as the test group, whereas animals of the other group fed on normal diet served as the control group. The rats were fed on respective diets for a period of 2 weeks after which stress was induced to half the animals of each group by i.p. administration of NDEA at 200 mg/kg body weight. Animals were killed 48 h post stress-induction period. Feed intake and body weight decreased significantly in both test and control groups, the effect being less in test group. Increase in osmotic fragility and in-vitro lipid peroxidation (LPO) on stress induction was of lower degree in the test group. NDEA toxicity was mainly reflected in liver as evidenced by increased activities of plasma aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase. The effect was of lower degree in test group as compared with that in the control group. Increase in urea levels observed following NDEA administration was also of lower degree in test groups. Blood glutathione (GSH) levels increased more so in test group compared with control group on stress induction. The activities of superoxide dismutase (SOD), peroxidase (Px), and catalase (CAT) activities decreased significantly on stress induction in erythrocytes. LPO increased in all the tissues through varying degree, and the increase was appreciably of lower degree in test group. The activity of SOD increased significantly in both test and control group on stress induction, whereas activities of Px and CAT decreased following NDEA treatment, and the effects were of lower degree in test group. Thus, supplementation of diet with thyme extract can improve antioxygenic potential and hence help to prevent oxidative stress. SN - 0960-3271 UR - https://www.unboundmedicine.com/medline/citation/18650253/Antioxidant_potential_of_thyme_extract:_alleviation_of_N_nitrosodiethylamine_induced_oxidative_stress_ L2 - http://journals.sagepub.com/doi/full/10.1177/0960327108088970?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -