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Potency of an inactivated avian influenza vaccine prepared from a non-pathogenic H5N1 reassortant virus generated between isolates from migratory ducks in Asia.
Arch Virol. 2008; 153(9):1685-92.AV

Abstract

A reassortant influenza virus, A/duck/Hokkaido/Vac-1/2004 (H5N1) (Dk/Vac-1/04), was generated between non-pathogenic avian influenza viruses isolated from migratory ducks in Asia. Dk/Vac-1/04 (H5N1) virus particles propagated in embryonated chicken eggs were inactivated with formalin and adjuvanted with mineral oil to form a water-in-oil emulsion. The resulting vaccine was injected intramuscularly into chickens. The chickens were challenged with either of the highly pathogenic avian influenza virus strains A/chicken/Yamaguchi/7/2004 (H5N1) or A/swan/Mongolia/3/2005 (H5N1) at 21 days post-vaccination (p. v.), when the geometric mean serum HI titers of the birds was 64 with the challenge virus strains. The vaccinated chickens were protected from manifestation of disease signs upon challenge with either of the highly pathogenic avian influenza viruses. However, challenge virus was recovered at low titers from the birds at 2 and 4 days post-challenge (p.c.). All 3 chickens challenged at 6 days p.v. died, whereas 3 chickens challenged at 8 days p.v. survived. These results indicate that the present vaccine confers clinical protection and reduction of virus shedding against highly pathogenic avian influenza virus challenge and should be useful as an optional tool in emergency cases.

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Authors+Show Affiliations

Isoda N
Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18-jyo Nishi-9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
Sakoda Y
No affiliation info available
Kishida N
No affiliation info available
Soda K
No affiliation info available
Sakabe S
No affiliation info available
Sakamoto R
No affiliation info available
Imamura T
No affiliation info available
Sakaguchi M
No affiliation info available
Sasaki T
No affiliation info available
Kokumai N
No affiliation info available
Ohgitani T
No affiliation info available
Saijo K
No affiliation info available
Sawata A
No affiliation info available
Hagiwara J
No affiliation info available
Lin Z
No affiliation info available
Kida H
No affiliation info available

MeSH

Animal MigrationAnimalsAnimals, WildAntibodies, ViralAsiaChick EmbryoChickensDucksInfluenza A Virus, H5N1 SubtypeInfluenza A virusInfluenza VaccinesInfluenza in BirdsReassortant VirusesVaccines, InactivatedVirus Shedding

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18651092

Citation

Isoda, Norikazu, et al. "Potency of an Inactivated Avian Influenza Vaccine Prepared From a Non-pathogenic H5N1 Reassortant Virus Generated Between Isolates From Migratory Ducks in Asia." Archives of Virology, vol. 153, no. 9, 2008, pp. 1685-92.
Isoda N, Sakoda Y, Kishida N, et al. Potency of an inactivated avian influenza vaccine prepared from a non-pathogenic H5N1 reassortant virus generated between isolates from migratory ducks in Asia. Arch Virol. 2008;153(9):1685-92.
Isoda, N., Sakoda, Y., Kishida, N., Soda, K., Sakabe, S., Sakamoto, R., Imamura, T., Sakaguchi, M., Sasaki, T., Kokumai, N., Ohgitani, T., Saijo, K., Sawata, A., Hagiwara, J., Lin, Z., & Kida, H. (2008). Potency of an inactivated avian influenza vaccine prepared from a non-pathogenic H5N1 reassortant virus generated between isolates from migratory ducks in Asia. Archives of Virology, 153(9), 1685-92. https://doi.org/10.1007/s00705-008-0171-1
Isoda N, et al. Potency of an Inactivated Avian Influenza Vaccine Prepared From a Non-pathogenic H5N1 Reassortant Virus Generated Between Isolates From Migratory Ducks in Asia. Arch Virol. 2008;153(9):1685-92. PubMed PMID: 18651092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potency of an inactivated avian influenza vaccine prepared from a non-pathogenic H5N1 reassortant virus generated between isolates from migratory ducks in Asia. AU - Isoda,Norikazu, AU - Sakoda,Yoshihiro, AU - Kishida,Noriko, AU - Soda,Kosuke, AU - Sakabe,Saori, AU - Sakamoto,Ryuichi, AU - Imamura,Takashi, AU - Sakaguchi,Masashi, AU - Sasaki,Takashi, AU - Kokumai,Norihide, AU - Ohgitani,Toshiaki, AU - Saijo,Kazue, AU - Sawata,Akira, AU - Hagiwara,Junko, AU - Lin,Zhifeng, AU - Kida,Hiroshi, Y1 - 2008/07/25/ PY - 2008/02/29/received PY - 2008/06/20/accepted PY - 2008/7/25/pubmed PY - 2008/11/1/medline PY - 2008/7/25/entrez SP - 1685 EP - 92 JF - Archives of virology JO - Arch Virol VL - 153 IS - 9 N2 - A reassortant influenza virus, A/duck/Hokkaido/Vac-1/2004 (H5N1) (Dk/Vac-1/04), was generated between non-pathogenic avian influenza viruses isolated from migratory ducks in Asia. Dk/Vac-1/04 (H5N1) virus particles propagated in embryonated chicken eggs were inactivated with formalin and adjuvanted with mineral oil to form a water-in-oil emulsion. The resulting vaccine was injected intramuscularly into chickens. The chickens were challenged with either of the highly pathogenic avian influenza virus strains A/chicken/Yamaguchi/7/2004 (H5N1) or A/swan/Mongolia/3/2005 (H5N1) at 21 days post-vaccination (p. v.), when the geometric mean serum HI titers of the birds was 64 with the challenge virus strains. The vaccinated chickens were protected from manifestation of disease signs upon challenge with either of the highly pathogenic avian influenza viruses. However, challenge virus was recovered at low titers from the birds at 2 and 4 days post-challenge (p.c.). All 3 chickens challenged at 6 days p.v. died, whereas 3 chickens challenged at 8 days p.v. survived. These results indicate that the present vaccine confers clinical protection and reduction of virus shedding against highly pathogenic avian influenza virus challenge and should be useful as an optional tool in emergency cases. SN - 0304-8608 UR - https://www.unboundmedicine.com/medline/citation/18651092/Potency_of_an_inactivated_avian_influenza_vaccine_prepared_from_a_non_pathogenic_H5N1_reassortant_virus_generated_between_isolates_from_migratory_ducks_in_Asia_ L2 - https://dx.doi.org/10.1007/s00705-008-0171-1 DB - PRIME DP - Unbound Medicine ER -