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Essential role of ADF/cofilin for assembly of contractile actin networks in the C. elegans somatic gonad.
J Cell Sci. 2008 Aug 15; 121(Pt 16):2662-70.JC

Abstract

The somatic gonad of the nematode Caenorhabditis elegans contains a myoepithelial sheath, which surrounds oocytes and provides contractile forces during ovulation. Contractile apparatuses of the myoepithelial-sheath cells are non-striated and similar to those of smooth muscle. We report the identification of a specific isoform of actin depolymerizing factor (ADF)/cofilin as an essential factor for assembly of contractile actin networks in the gonadal myoepithelial sheath. Two ADF/cofilin isoforms, UNC-60A and UNC-60B, are expressed from the unc-60 gene by alternative splicing. RNA interference of UNC-60A caused disorganization of the actin networks in the myoepithelial sheath. UNC-60B, which is known to function in the body-wall muscle, was not necessary or sufficient for actin organization in the myoepithelial sheath. However, mutant forms of UNC-60B with reduced actin-filament-severing activity rescued the UNC-60A-depletion phenotype. UNC-60A has a much weaker filament-severing activity than UNC-60B, suggesting that an ADF/cofilin with weak severing activity is optimal for assembly of actin networks in the myoepithelial sheath. By contrast, strong actin-filament-severing activity of UNC-60B was required for assembly of striated myofibrils in the body-wall muscle. Our results suggest that an optimal level of actin-filament-severing activity of ADF/cofilin is required for assembly of actin networks in the somatic gonad.

Authors+Show Affiliations

Department of Pathology, Emory University, Atlanta, GA 30322, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18653537

Citation

Ono, Kanako, et al. "Essential Role of ADF/cofilin for Assembly of Contractile Actin Networks in the C. Elegans Somatic Gonad." Journal of Cell Science, vol. 121, no. Pt 16, 2008, pp. 2662-70.
Ono K, Yamashiro S, Ono S. Essential role of ADF/cofilin for assembly of contractile actin networks in the C. elegans somatic gonad. J Cell Sci. 2008;121(Pt 16):2662-70.
Ono, K., Yamashiro, S., & Ono, S. (2008). Essential role of ADF/cofilin for assembly of contractile actin networks in the C. elegans somatic gonad. Journal of Cell Science, 121(Pt 16), 2662-70. https://doi.org/10.1242/jcs.034215
Ono K, Yamashiro S, Ono S. Essential Role of ADF/cofilin for Assembly of Contractile Actin Networks in the C. Elegans Somatic Gonad. J Cell Sci. 2008 Aug 15;121(Pt 16):2662-70. PubMed PMID: 18653537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Essential role of ADF/cofilin for assembly of contractile actin networks in the C. elegans somatic gonad. AU - Ono,Kanako, AU - Yamashiro,Sawako, AU - Ono,Shoichiro, Y1 - 2008/07/24/ PY - 2008/7/26/pubmed PY - 2008/11/5/medline PY - 2008/7/26/entrez SP - 2662 EP - 70 JF - Journal of cell science JO - J Cell Sci VL - 121 IS - Pt 16 N2 - The somatic gonad of the nematode Caenorhabditis elegans contains a myoepithelial sheath, which surrounds oocytes and provides contractile forces during ovulation. Contractile apparatuses of the myoepithelial-sheath cells are non-striated and similar to those of smooth muscle. We report the identification of a specific isoform of actin depolymerizing factor (ADF)/cofilin as an essential factor for assembly of contractile actin networks in the gonadal myoepithelial sheath. Two ADF/cofilin isoforms, UNC-60A and UNC-60B, are expressed from the unc-60 gene by alternative splicing. RNA interference of UNC-60A caused disorganization of the actin networks in the myoepithelial sheath. UNC-60B, which is known to function in the body-wall muscle, was not necessary or sufficient for actin organization in the myoepithelial sheath. However, mutant forms of UNC-60B with reduced actin-filament-severing activity rescued the UNC-60A-depletion phenotype. UNC-60A has a much weaker filament-severing activity than UNC-60B, suggesting that an ADF/cofilin with weak severing activity is optimal for assembly of actin networks in the myoepithelial sheath. By contrast, strong actin-filament-severing activity of UNC-60B was required for assembly of striated myofibrils in the body-wall muscle. Our results suggest that an optimal level of actin-filament-severing activity of ADF/cofilin is required for assembly of actin networks in the somatic gonad. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/18653537/Essential_role_of_ADF/cofilin_for_assembly_of_contractile_actin_networks_in_the_C__elegans_somatic_gonad_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=18653537 DB - PRIME DP - Unbound Medicine ER -