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Essential fatty acid deficiency in mice impairs lactose digestion.
Am J Physiol Gastrointest Liver Physiol. 2008 Sep; 295(3):G605-13.AJ

Abstract

Essential fatty acid (EFA) deficiency in mice induces fat malabsorption. We previously reported indications that the underlying mechanism is located at the level of the intestinal mucosa. We have investigated the effects of EFA deficiency on small intestinal morphology and function. Mice were fed an EFA-deficient or control diet for 8 wk. A 72-h fat balance, the EFA status, and small intestinal histology were determined. Carbohydrate absorptive and digestive capacities were assessed by stable isotope methodology after administration of [U-(13)C]glucose and [1-(13)C]lactose. The mRNA expression and enzyme activity of lactase, and concentrations of the EFA linoleic acid (LA) were measured in small intestinal mucosa. Mice fed the EFA-deficient diet were markedly EFA-deficient with a profound fat malabsorption. EFA deficiency did not affect the histology or proliferative capacity of the small intestine. Blood [13C6]glucose appearance and disappearance were similar in both groups, indicating unaffected monosaccharide absorption. In contrast, blood appearance of [13C]glucose, originating from [1-(13)C]lactose, was delayed in EFA-deficient mice. EFA deficiency profoundly reduced lactase activity (-58%, P<0.01) and mRNA expression (-55%, P<0.01) in mid-small intestine. Both lactase activity and its mRNA expression strongly correlated with mucosal LA concentrations (r=0.77 and 0.79, respectively, P<0.01). EFA deficiency in mice inhibits the capacity to digest lactose but does not affect small intestinal histology. These data underscore the observation that EFA deficiency functionally impairs the small intestine, which in part may be mediated by low LA levels in the enterocytes.

Authors+Show Affiliations

Pediatric Gastroenterology, Department of Pediatrics, Beatrix Children's Hospital, Groningen University Institute for Drug Exploration, Center for Liver, Digestive and Metabolic Diseases, Groningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18653724

Citation

Lukovac, S, et al. "Essential Fatty Acid Deficiency in Mice Impairs Lactose Digestion." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 295, no. 3, 2008, pp. G605-13.
Lukovac S, Los EL, Stellaard F, et al. Essential fatty acid deficiency in mice impairs lactose digestion. Am J Physiol Gastrointest Liver Physiol. 2008;295(3):G605-13.
Lukovac, S., Los, E. L., Stellaard, F., Rings, E. H., & Verkade, H. J. (2008). Essential fatty acid deficiency in mice impairs lactose digestion. American Journal of Physiology. Gastrointestinal and Liver Physiology, 295(3), G605-13. https://doi.org/10.1152/ajpgi.90206.2008
Lukovac S, et al. Essential Fatty Acid Deficiency in Mice Impairs Lactose Digestion. Am J Physiol Gastrointest Liver Physiol. 2008;295(3):G605-13. PubMed PMID: 18653724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Essential fatty acid deficiency in mice impairs lactose digestion. AU - Lukovac,S, AU - Los,E L, AU - Stellaard,F, AU - Rings,E H H M, AU - Verkade,H J, Y1 - 2008/07/24/ PY - 2008/7/26/pubmed PY - 2008/10/10/medline PY - 2008/7/26/entrez SP - G605 EP - 13 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 295 IS - 3 N2 - Essential fatty acid (EFA) deficiency in mice induces fat malabsorption. We previously reported indications that the underlying mechanism is located at the level of the intestinal mucosa. We have investigated the effects of EFA deficiency on small intestinal morphology and function. Mice were fed an EFA-deficient or control diet for 8 wk. A 72-h fat balance, the EFA status, and small intestinal histology were determined. Carbohydrate absorptive and digestive capacities were assessed by stable isotope methodology after administration of [U-(13)C]glucose and [1-(13)C]lactose. The mRNA expression and enzyme activity of lactase, and concentrations of the EFA linoleic acid (LA) were measured in small intestinal mucosa. Mice fed the EFA-deficient diet were markedly EFA-deficient with a profound fat malabsorption. EFA deficiency did not affect the histology or proliferative capacity of the small intestine. Blood [13C6]glucose appearance and disappearance were similar in both groups, indicating unaffected monosaccharide absorption. In contrast, blood appearance of [13C]glucose, originating from [1-(13)C]lactose, was delayed in EFA-deficient mice. EFA deficiency profoundly reduced lactase activity (-58%, P<0.01) and mRNA expression (-55%, P<0.01) in mid-small intestine. Both lactase activity and its mRNA expression strongly correlated with mucosal LA concentrations (r=0.77 and 0.79, respectively, P<0.01). EFA deficiency in mice inhibits the capacity to digest lactose but does not affect small intestinal histology. These data underscore the observation that EFA deficiency functionally impairs the small intestine, which in part may be mediated by low LA levels in the enterocytes. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/18653724/Essential_fatty_acid_deficiency_in_mice_impairs_lactose_digestion_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.90206.2008?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -