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Rapid exercise-induced changes in PGC-1alpha mRNA and protein in human skeletal muscle.
J Appl Physiol (1985). 2008 Oct; 105(4):1098-105.JA

Abstract

The mRNA of the nuclear coactivator peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) increases during prolonged exercise and is influenced by carbohydrate availability. It is unknown if the increases in mRNA reflect the PGC-1alpha protein or if glycogen stores are an important regulator. Seven male subjects [23 +/- 1.3 yr old, maximum oxygen uptake (Vo(2 max)) 48.4 +/- 0.8 ml.kg(-1).min(-1)] exercised to exhaustion (approximately 2 h) at 65% Vo(2 max) followed by ingestion of either a high-carbohydrate (HC) or low-carbohydrate (LC) diet (7 or 2.9 g.kg(-1).day(-1), respectively) for 52 h of recovery. Glycogen remained depressed in LC (P < 0.05) while returning to resting levels by 24 h in HC. PGC-1alpha mRNA increased both at exhaustion (3-fold) and 2 h later (6.2-fold) (P < 0.05) but returned to rest levels by 24 h. PGC-1alpha protein increased (P < 0.05) 23% at exhaustion and remained elevated for at least 24 h (P < 0.05). While there was no direct treatment effect (HC vs. LC) for PGC-1alpha mRNA or protein, there was a linear relationship between the changes in glycogen and those in PGC-1alpha protein during exercise and recovery (r = -0.68, P < 0.05). In contrast, PGC-1beta did not increase with exercise but rather decreased (P < 0.05) below rest level at 24 and 52 h, and the decrease was greater (P < 0.05) in LC. PGC-1alpha protein content increased in prolonged exercise and remained upregulated for 24 h, but this could not have been predicted by the changes in mRNA. The beta-isoform declined rather than increasing, and this was greater when glycogen was not resynthesized to rest levels.

Authors+Show Affiliations

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18653753

Citation

Mathai, Anila S., et al. "Rapid Exercise-induced Changes in PGC-1alpha mRNA and Protein in Human Skeletal Muscle." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 105, no. 4, 2008, pp. 1098-105.
Mathai AS, Bonen A, Benton CR, et al. Rapid exercise-induced changes in PGC-1alpha mRNA and protein in human skeletal muscle. J Appl Physiol. 2008;105(4):1098-105.
Mathai, A. S., Bonen, A., Benton, C. R., Robinson, D. L., & Graham, T. E. (2008). Rapid exercise-induced changes in PGC-1alpha mRNA and protein in human skeletal muscle. Journal of Applied Physiology (Bethesda, Md. : 1985), 105(4), 1098-105. https://doi.org/10.1152/japplphysiol.00847.2007
Mathai AS, et al. Rapid Exercise-induced Changes in PGC-1alpha mRNA and Protein in Human Skeletal Muscle. J Appl Physiol. 2008;105(4):1098-105. PubMed PMID: 18653753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid exercise-induced changes in PGC-1alpha mRNA and protein in human skeletal muscle. AU - Mathai,Anila S, AU - Bonen,Arend, AU - Benton,Carley R, AU - Robinson,D L, AU - Graham,Terry E, Y1 - 2008/07/24/ PY - 2008/7/26/pubmed PY - 2008/11/7/medline PY - 2008/7/26/entrez SP - 1098 EP - 105 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J. Appl. Physiol. VL - 105 IS - 4 N2 - The mRNA of the nuclear coactivator peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) increases during prolonged exercise and is influenced by carbohydrate availability. It is unknown if the increases in mRNA reflect the PGC-1alpha protein or if glycogen stores are an important regulator. Seven male subjects [23 +/- 1.3 yr old, maximum oxygen uptake (Vo(2 max)) 48.4 +/- 0.8 ml.kg(-1).min(-1)] exercised to exhaustion (approximately 2 h) at 65% Vo(2 max) followed by ingestion of either a high-carbohydrate (HC) or low-carbohydrate (LC) diet (7 or 2.9 g.kg(-1).day(-1), respectively) for 52 h of recovery. Glycogen remained depressed in LC (P < 0.05) while returning to resting levels by 24 h in HC. PGC-1alpha mRNA increased both at exhaustion (3-fold) and 2 h later (6.2-fold) (P < 0.05) but returned to rest levels by 24 h. PGC-1alpha protein increased (P < 0.05) 23% at exhaustion and remained elevated for at least 24 h (P < 0.05). While there was no direct treatment effect (HC vs. LC) for PGC-1alpha mRNA or protein, there was a linear relationship between the changes in glycogen and those in PGC-1alpha protein during exercise and recovery (r = -0.68, P < 0.05). In contrast, PGC-1beta did not increase with exercise but rather decreased (P < 0.05) below rest level at 24 and 52 h, and the decrease was greater (P < 0.05) in LC. PGC-1alpha protein content increased in prolonged exercise and remained upregulated for 24 h, but this could not have been predicted by the changes in mRNA. The beta-isoform declined rather than increasing, and this was greater when glycogen was not resynthesized to rest levels. SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/18653753/Rapid_exercise_induced_changes_in_PGC_1alpha_mRNA_and_protein_in_human_skeletal_muscle_ L2 - http://www.physiology.org/doi/full/10.1152/japplphysiol.00847.2007?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -