TY - JOUR T1 - Identification and quantitation of auxins in plants by liquid chromatography/electrospray ionization ion trap mass spectrometry. AU - Lu,Qiaomei, AU - Zhang,Lan, AU - Chen,Tianwen, AU - Lu,Minghua, AU - Ping,Tong, AU - Chen,Guonan, PY - 2008/7/26/pubmed PY - 2008/9/13/medline PY - 2008/7/26/entrez SP - 2565 EP - 72 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 16 N2 - Auxin is an important phylohormone, which regulates specific physiological responses such as division, elongation and differentiation of cells. A new method using liquid chromatography/electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) has been developed for identification and quantitation of four auxins. Under the optimum conditions, four auxins (indole-3-acetic acid, indole-3-propionic acid, indole-3-butyric acid and 1-naphthylacetic acid) were completely separated and quantitated within 7 min with a minimum detection limit of 8.0 ng mL(-1) with relative standard deviations lower than 5.0%. This method also has been applied to analysis of auxins in Chinese cabbage where, even with a complicated serious background perturbation due to the natural biological matrix, the mean recoveries ranged from 77.5% to 99.8%. Finally, we discuss the MS-relevant properties of the identified auxins in detail. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18655000/Identification_and_quantitation_of_auxins_in_plants_by_liquid_chromatography/electrospray_ionization_ion_trap_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3642 DB - PRIME DP - Unbound Medicine ER -