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Simultaneous detection of six human diarrheal pathogens by using DNA microarray combined with tyramide signal amplification.
J Microbiol Methods. 2008 Oct; 75(2):365-8.JM

Abstract

Multiplex PCR and DNA microarray were combined with tyramide signal amplification (TSA) to develop a reliable method suitable for simultaneous detection of six species of human diarrheal pathogens (Yersinia enterocolitica, Shigella spp, Salmonella typhi, Brucella spp, Vibrio cholera and Escherichia coli O157:H7). Meanwhile, our method could distinguish V. cholera serotype O1 from O139, and O157:H7 from O157: non-H7. This assay conferred a specificity of 100% for target pathogens. The limit of detection was 103 degrees CFU/mL approximately. The results of 98.6% (357/362) clinical specimens and 100% (5/5) mocked double-blind samples were the same to that from conventional assay. Consequently this assay is sensitive and a specific tool suitable for diagnostic detection and surveillance of multiple human pathogens.

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Authors+Show Affiliations

Jin D
Beijing Institute of Radiation Medicine, No. 27 Taiping road, Beijing, China. dazhijin_y@hotmail.com
Qi H
No affiliation info available
Chen S
No affiliation info available
Zeng T
No affiliation info available
Liu Q
No affiliation info available
Wang S
No affiliation info available

MeSH

DNA ProbesDiarrheaGram-Negative BacteriaGram-Negative Bacterial InfectionsHumansOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionSensitivity and SpecificityTyramine

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18655810

Citation

Jin, Dazhi, et al. "Simultaneous Detection of Six Human Diarrheal Pathogens By Using DNA Microarray Combined With Tyramide Signal Amplification." Journal of Microbiological Methods, vol. 75, no. 2, 2008, pp. 365-8.
Jin D, Qi H, Chen S, et al. Simultaneous detection of six human diarrheal pathogens by using DNA microarray combined with tyramide signal amplification. J Microbiol Methods. 2008;75(2):365-8.
Jin, D., Qi, H., Chen, S., Zeng, T., Liu, Q., & Wang, S. (2008). Simultaneous detection of six human diarrheal pathogens by using DNA microarray combined with tyramide signal amplification. Journal of Microbiological Methods, 75(2), 365-8. https://doi.org/10.1016/j.mimet.2008.06.020
Jin D, et al. Simultaneous Detection of Six Human Diarrheal Pathogens By Using DNA Microarray Combined With Tyramide Signal Amplification. J Microbiol Methods. 2008;75(2):365-8. PubMed PMID: 18655810.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous detection of six human diarrheal pathogens by using DNA microarray combined with tyramide signal amplification. AU - Jin,Dazhi, AU - Qi,Hongjuan, AU - Chen,Suhong, AU - Zeng,Ting, AU - Liu,Qiqi, AU - Wang,Shengqi, Y1 - 2008/07/02/ PY - 2007/12/21/received PY - 2008/06/18/revised PY - 2008/06/20/accepted PY - 2008/7/29/pubmed PY - 2008/12/17/medline PY - 2008/7/29/entrez SP - 365 EP - 8 JF - Journal of microbiological methods JO - J Microbiol Methods VL - 75 IS - 2 N2 - Multiplex PCR and DNA microarray were combined with tyramide signal amplification (TSA) to develop a reliable method suitable for simultaneous detection of six species of human diarrheal pathogens (Yersinia enterocolitica, Shigella spp, Salmonella typhi, Brucella spp, Vibrio cholera and Escherichia coli O157:H7). Meanwhile, our method could distinguish V. cholera serotype O1 from O139, and O157:H7 from O157: non-H7. This assay conferred a specificity of 100% for target pathogens. The limit of detection was 103 degrees CFU/mL approximately. The results of 98.6% (357/362) clinical specimens and 100% (5/5) mocked double-blind samples were the same to that from conventional assay. Consequently this assay is sensitive and a specific tool suitable for diagnostic detection and surveillance of multiple human pathogens. SN - 0167-7012 UR - https://www.unboundmedicine.com/medline/citation/18655810/Simultaneous_detection_of_six_human_diarrheal_pathogens_by_using_DNA_microarray_combined_with_tyramide_signal_amplification_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-7012(08)00245-5 DB - PRIME DP - Unbound Medicine ER -