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Caffeic acid phenethyl ester prevents cerebellar granule neurons (CGNs) against glutamate-induced neurotoxicity.
Neuroscience. 2008 Sep 09; 155(4):1098-105.N

Abstract

Caffeic acid phenethyl ester (CAPE) is an active component of propolis obtained from honeybee hives and is found to have the following properties: anti-mitogenic, anti-carcinogenic, anti-inflammatory, immunomodulatory, and antioxidant. Recent reports suggest that CAPE also has a neuronal protective property against ischemic injury. Since excitotoxicity may play an important role in ischemia, in this study, we investigated whether CAPE could directly protect neurons against excitotoxic insult. We treated cultured rat cerebellar granule neurons (CGNs) with excitotoxic concentrations of glutamate in the presence or absence of CAPE and found that CAPE markedly protected neurons against glutamate-induced neuronal death in a concentration-dependent fashion. Glutamate-induced CGNs death is associated with time-dependent activation of caspase-3 and phosphorylation of p38, both events of which can be blocked by CAPE. Treating CGNs with specific inhibitors of these two enzymes together exerts a synergistic neuroprotective effect, similar to the neuroprotective effect of CAPE exposure. These results suggest that CAPE is able to block glutamate-induced excitotoxicity by inhibiting phosphorylation of p38 and caspase-3 activation. This finding may further help understanding of the mechanism of glutamate-induced neuronal death and CAPE-induced neuroprotection against excitotoxicity.

Authors+Show Affiliations

Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18657598

Citation

Wei, X, et al. "Caffeic Acid Phenethyl Ester Prevents Cerebellar Granule Neurons (CGNs) Against Glutamate-induced Neurotoxicity." Neuroscience, vol. 155, no. 4, 2008, pp. 1098-105.
Wei X, Ma Z, Fontanilla CV, et al. Caffeic acid phenethyl ester prevents cerebellar granule neurons (CGNs) against glutamate-induced neurotoxicity. Neuroscience. 2008;155(4):1098-105.
Wei, X., Ma, Z., Fontanilla, C. V., Zhao, L., Xu, Z. C., Taggliabraci, V., Johnstone, B. H., Dodel, R. C., Farlow, M. R., & Du, Y. (2008). Caffeic acid phenethyl ester prevents cerebellar granule neurons (CGNs) against glutamate-induced neurotoxicity. Neuroscience, 155(4), 1098-105. https://doi.org/10.1016/j.neuroscience.2008.06.056
Wei X, et al. Caffeic Acid Phenethyl Ester Prevents Cerebellar Granule Neurons (CGNs) Against Glutamate-induced Neurotoxicity. Neuroscience. 2008 Sep 9;155(4):1098-105. PubMed PMID: 18657598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caffeic acid phenethyl ester prevents cerebellar granule neurons (CGNs) against glutamate-induced neurotoxicity. AU - Wei,X, AU - Ma,Z, AU - Fontanilla,C V, AU - Zhao,L, AU - Xu,Z C, AU - Taggliabraci,V, AU - Johnstone,B H, AU - Dodel,R C, AU - Farlow,M R, AU - Du,Y, Y1 - 2008/07/03/ PY - 2008/04/03/received PY - 2008/05/27/revised PY - 2008/06/03/accepted PY - 2008/7/29/pubmed PY - 2009/2/7/medline PY - 2008/7/29/entrez SP - 1098 EP - 105 JF - Neuroscience JO - Neuroscience VL - 155 IS - 4 N2 - Caffeic acid phenethyl ester (CAPE) is an active component of propolis obtained from honeybee hives and is found to have the following properties: anti-mitogenic, anti-carcinogenic, anti-inflammatory, immunomodulatory, and antioxidant. Recent reports suggest that CAPE also has a neuronal protective property against ischemic injury. Since excitotoxicity may play an important role in ischemia, in this study, we investigated whether CAPE could directly protect neurons against excitotoxic insult. We treated cultured rat cerebellar granule neurons (CGNs) with excitotoxic concentrations of glutamate in the presence or absence of CAPE and found that CAPE markedly protected neurons against glutamate-induced neuronal death in a concentration-dependent fashion. Glutamate-induced CGNs death is associated with time-dependent activation of caspase-3 and phosphorylation of p38, both events of which can be blocked by CAPE. Treating CGNs with specific inhibitors of these two enzymes together exerts a synergistic neuroprotective effect, similar to the neuroprotective effect of CAPE exposure. These results suggest that CAPE is able to block glutamate-induced excitotoxicity by inhibiting phosphorylation of p38 and caspase-3 activation. This finding may further help understanding of the mechanism of glutamate-induced neuronal death and CAPE-induced neuroprotection against excitotoxicity. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/18657598/Caffeic_acid_phenethyl_ester_prevents_cerebellar_granule_neurons__CGNs__against_glutamate_induced_neurotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(08)00877-4 DB - PRIME DP - Unbound Medicine ER -