Tags

Type your tag names separated by a space and hit enter

[Study on HPLC fingerprint of Gentiana macrophylla].
Zhong Yao Cai. 2008 Apr; 31(4):498-501.ZY

Abstract

OBJECTIVE

To establish HPLC fingerprint of Gentiana macrophylla, which was expected to be standards of quality control and identification of the Chinese crude drug.

METHODS

The HPLC method was used, chromatography conditions were Shimadzu C18 (150 mm x 4.6 mm, 5 microm) column with gradient mobile phase of methanol-0.4% Phosphoric acid, UV detection wavelength was at 230 nm and the column temperature was at 25 degrees C with the flow rate of 1.0 ml/min.

RESULTS

5 peaks were identified as the characteristic fingerprints of Gentiana macrophylla and the fingerprints of the 14 samples had high similarities. The similarities of disqualified and fake medicinal materials were low.

CONCLUSION

This HPLC fingerprint of Gentiana macrophylla can be used as a standard of quality control and identification of the Chinese crude drug.

Authors+Show Affiliations

Cao XY
Key Laboratory of Medicinal Plant Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shanxi Normal University, Xi'an 710062, China. caoxiaoyan@snnu.edu.cn
Li YH
No affiliation info available
Wang ZZ
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDrug ContaminationDrugs, Chinese HerbalGentianaceaeMethanolPlant RootsPlants, MedicinalQuality ControlReproducibility of Results

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

18661818

Citation

Cao, Xiao-Yan, et al. "[Study On HPLC Fingerprint of Gentiana Macrophylla]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 31, no. 4, 2008, pp. 498-501.
Cao XY, Li YH, Wang ZZ. [Study on HPLC fingerprint of Gentiana macrophylla]. Zhong Yao Cai. 2008;31(4):498-501.
Cao, X. Y., Li, Y. H., & Wang, Z. Z. (2008). [Study on HPLC fingerprint of Gentiana macrophylla]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 31(4), 498-501.
Cao XY, Li YH, Wang ZZ. [Study On HPLC Fingerprint of Gentiana Macrophylla]. Zhong Yao Cai. 2008;31(4):498-501. PubMed PMID: 18661818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on HPLC fingerprint of Gentiana macrophylla]. AU - Cao,Xiao-Yan, AU - Li,Yong-Hui, AU - Wang,Zhe-Zhi, PY - 2008/7/30/pubmed PY - 2009/10/14/medline PY - 2008/7/30/entrez SP - 498 EP - 501 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 31 IS - 4 N2 - OBJECTIVE: To establish HPLC fingerprint of Gentiana macrophylla, which was expected to be standards of quality control and identification of the Chinese crude drug. METHODS: The HPLC method was used, chromatography conditions were Shimadzu C18 (150 mm x 4.6 mm, 5 microm) column with gradient mobile phase of methanol-0.4% Phosphoric acid, UV detection wavelength was at 230 nm and the column temperature was at 25 degrees C with the flow rate of 1.0 ml/min. RESULTS: 5 peaks were identified as the characteristic fingerprints of Gentiana macrophylla and the fingerprints of the 14 samples had high similarities. The similarities of disqualified and fake medicinal materials were low. CONCLUSION: This HPLC fingerprint of Gentiana macrophylla can be used as a standard of quality control and identification of the Chinese crude drug. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/18661818/[Study_on_HPLC_fingerprint_of_Gentiana_macrophylla]_ DB - PRIME DP - Unbound Medicine ER -