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Glycyrrhizin reduces secondary inflammatory process after spinal cord compression injury in mice.
Shock. 2009 Apr; 31(4):367-75.S

Abstract

Glycyrrhizin, a major active constituent of liquorice root (Glycyrrhiza glabra), has a free radical scavenging property, and its effects were evaluated on an animal model of spinal cord injury (SCI) induced by the application of vascular clips (force of 24 g) to the dura via a four-level T5-T8 laminectomy. Spinal cord injury in mice resulted in severe trauma characterized by edema, tissue damage, and apoptosis (measured by terminal deoxynucleotidyltransferase-mediated dUTP-biotin end labeling staining, Bax, and Bcl-2 expression). Immunohistochemical examination demonstrated a marked increase in immunoreactivity for nitrotyrosine, iNOS, and poly(adenosine diphosphate-ribose) in the spinal cord tissue. Additionally, we demonstrate that these inflammatory events were associated with the activation of nuclear factor-kappaB. In contrast, the degree of (1) spinal cord inflammation and tissue injury (histological score), (2) nitrotyrosine and poly(adenosine diphosphate [ADP] ribose) formation, (3) iNOS expression, (4) nuclear factor-kappaB activation, and (5) apoptosis (terminal deoxynucleotidyltransferase-mediated dUTP-biotin end labeling, Bax, and Bcl-2) was markedly reduced in spinal cord tissue obtained from mice treated with glycyrrhizin extract (10 mg/kg, i.p., 30 min before and 1 and 6 h after SCI). In a separate set of experiments, we have clearly demonstrated that glycyrrhizin extract treatment significantly ameliorated the recovery of limb function (evaluated by motor recovery score). Taken together, our results clearly demonstrate that treatment with glycyrrhizin extract reduces the development of inflammation and tissue injury events associated with spinal cord trauma.

Authors+Show Affiliations

IRCCS Centro Neurolesi Bonino-Pulejo, Messina, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18665052

Citation

Genovese, Tiziana, et al. "Glycyrrhizin Reduces Secondary Inflammatory Process After Spinal Cord Compression Injury in Mice." Shock (Augusta, Ga.), vol. 31, no. 4, 2009, pp. 367-75.
Genovese T, Menegazzi M, Mazzon E, et al. Glycyrrhizin reduces secondary inflammatory process after spinal cord compression injury in mice. Shock. 2009;31(4):367-75.
Genovese, T., Menegazzi, M., Mazzon, E., Crisafulli, C., Di Paola, R., Dal Bosco, M., Zou, Z., Suzuki, H., & Cuzzocrea, S. (2009). Glycyrrhizin reduces secondary inflammatory process after spinal cord compression injury in mice. Shock (Augusta, Ga.), 31(4), 367-75. https://doi.org/10.1097/SHK.0b013e3181833b08
Genovese T, et al. Glycyrrhizin Reduces Secondary Inflammatory Process After Spinal Cord Compression Injury in Mice. Shock. 2009;31(4):367-75. PubMed PMID: 18665052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glycyrrhizin reduces secondary inflammatory process after spinal cord compression injury in mice. AU - Genovese,Tiziana, AU - Menegazzi,Marta, AU - Mazzon,Emanuela, AU - Crisafulli,Concetta, AU - Di Paola,Rosanna, AU - Dal Bosco,Martina, AU - Zou,Zhenzhen, AU - Suzuki,Hisanori, AU - Cuzzocrea,Salvatore, PY - 2008/7/31/pubmed PY - 2009/5/21/medline PY - 2008/7/31/entrez SP - 367 EP - 75 JF - Shock (Augusta, Ga.) JO - Shock VL - 31 IS - 4 N2 - Glycyrrhizin, a major active constituent of liquorice root (Glycyrrhiza glabra), has a free radical scavenging property, and its effects were evaluated on an animal model of spinal cord injury (SCI) induced by the application of vascular clips (force of 24 g) to the dura via a four-level T5-T8 laminectomy. Spinal cord injury in mice resulted in severe trauma characterized by edema, tissue damage, and apoptosis (measured by terminal deoxynucleotidyltransferase-mediated dUTP-biotin end labeling staining, Bax, and Bcl-2 expression). Immunohistochemical examination demonstrated a marked increase in immunoreactivity for nitrotyrosine, iNOS, and poly(adenosine diphosphate-ribose) in the spinal cord tissue. Additionally, we demonstrate that these inflammatory events were associated with the activation of nuclear factor-kappaB. In contrast, the degree of (1) spinal cord inflammation and tissue injury (histological score), (2) nitrotyrosine and poly(adenosine diphosphate [ADP] ribose) formation, (3) iNOS expression, (4) nuclear factor-kappaB activation, and (5) apoptosis (terminal deoxynucleotidyltransferase-mediated dUTP-biotin end labeling, Bax, and Bcl-2) was markedly reduced in spinal cord tissue obtained from mice treated with glycyrrhizin extract (10 mg/kg, i.p., 30 min before and 1 and 6 h after SCI). In a separate set of experiments, we have clearly demonstrated that glycyrrhizin extract treatment significantly ameliorated the recovery of limb function (evaluated by motor recovery score). Taken together, our results clearly demonstrate that treatment with glycyrrhizin extract reduces the development of inflammation and tissue injury events associated with spinal cord trauma. SN - 1540-0514 UR - https://www.unboundmedicine.com/medline/citation/18665052/Glycyrrhizin_reduces_secondary_inflammatory_process_after_spinal_cord_compression_injury_in_mice_ L2 - https://doi.org/10.1097/SHK.0b013e3181833b08 DB - PRIME DP - Unbound Medicine ER -