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SPE and HPLC/UV of resin acids in colophonium-containing products.
J Sep Sci. 2008 Aug; 31(15):2784-90.JS

Abstract

A new method, involving SPE and HPLC/UV diode-array detection (DAD), was developed for the quantification of colophonium components in different consumer products, such as cosmetics. Colophonium is a common cause of contact dermatitis since its components can oxidize into allergens on exposure to air. Three different resin acids were used as markers for native and oxidized colophonium, abietic acid (AbA), dehydroabietic acid (DeA), and 7-oxodehydroabietic acid (7-O-DeA). The SPE method, utilizing a mixed-mode hydrophobic and anion exchange retention mechanism, was shown to yield very clean extracts. The use of a urea-embedded C(12) HPLC stationary phase improved the separation of the resin acids compared to common C(18). Concentrations higher than 2 mg/g of both AbA and DeA were detected in wax strips. In this product also 7-O-DeA, a marker for oxidized colophonium, was detected at a level of 28 microg/g. The LODs were in the range of 7-19 microg/g and the LOQs 22-56 microg/g. The method is simple to use and can be applied on many types of technical products, not only cosmetics. For the first time, a method for technical products was developed, which separates AbA from pimaric acid.

Authors+Show Affiliations

Department of Analytical Chemistry, Stockholm University, Stockholm, Sweden. ulrika.nilsson@anchem.su.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18666177

Citation

Nilsson, Ulrika, et al. "SPE and HPLC/UV of Resin Acids in Colophonium-containing Products." Journal of Separation Science, vol. 31, no. 15, 2008, pp. 2784-90.
Nilsson U, Berglund N, Lindahl F, et al. SPE and HPLC/UV of resin acids in colophonium-containing products. J Sep Sci. 2008;31(15):2784-90.
Nilsson, U., Berglund, N., Lindahl, F., Axelsson, S., Redeby, T., Lassen, P., & Karlberg, A. T. (2008). SPE and HPLC/UV of resin acids in colophonium-containing products. Journal of Separation Science, 31(15), 2784-90. https://doi.org/10.1002/jssc.200800210
Nilsson U, et al. SPE and HPLC/UV of Resin Acids in Colophonium-containing Products. J Sep Sci. 2008;31(15):2784-90. PubMed PMID: 18666177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SPE and HPLC/UV of resin acids in colophonium-containing products. AU - Nilsson,Ulrika, AU - Berglund,Naghmeh, AU - Lindahl,Fredrik, AU - Axelsson,Sara, AU - Redeby,Theres, AU - Lassen,Pia, AU - Karlberg,Ann-Therese, PY - 2008/7/31/pubmed PY - 2008/11/8/medline PY - 2008/7/31/entrez SP - 2784 EP - 90 JF - Journal of separation science JO - J Sep Sci VL - 31 IS - 15 N2 - A new method, involving SPE and HPLC/UV diode-array detection (DAD), was developed for the quantification of colophonium components in different consumer products, such as cosmetics. Colophonium is a common cause of contact dermatitis since its components can oxidize into allergens on exposure to air. Three different resin acids were used as markers for native and oxidized colophonium, abietic acid (AbA), dehydroabietic acid (DeA), and 7-oxodehydroabietic acid (7-O-DeA). The SPE method, utilizing a mixed-mode hydrophobic and anion exchange retention mechanism, was shown to yield very clean extracts. The use of a urea-embedded C(12) HPLC stationary phase improved the separation of the resin acids compared to common C(18). Concentrations higher than 2 mg/g of both AbA and DeA were detected in wax strips. In this product also 7-O-DeA, a marker for oxidized colophonium, was detected at a level of 28 microg/g. The LODs were in the range of 7-19 microg/g and the LOQs 22-56 microg/g. The method is simple to use and can be applied on many types of technical products, not only cosmetics. For the first time, a method for technical products was developed, which separates AbA from pimaric acid. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/18666177/SPE_and_HPLC/UV_of_resin_acids_in_colophonium_containing_products_ L2 - https://doi.org/10.1002/jssc.200800210 DB - PRIME DP - Unbound Medicine ER -