Tags

Type your tag names separated by a space and hit enter

A new aptameric biosensor for cocaine based on surface-enhanced Raman scattering spectroscopy.
Chemistry. 2008; 14(27):8374-82.C

Abstract

The present study reports the proof of principle of a reagentless aptameric sensor based on surface-enhanced Raman scattering (SERS) spectroscopy with "signal-on" architecture using a model target of cocaine. This new aptameric sensor is based on the conformational change of the surface-tethered aptamer on a binding target that draws a certain Raman reporter in close proximity to the SERS substrate, thereby increasing the Raman scattering signal due to the local enhancement effect of SERS. To improve the response performance, the sensor is fabricated from a cocaine-templated mixed self-assembly of a 3'-terminal tetramethylrhodamine (TMR)-labeled DNA aptamer on a silver colloid film by means of an alkanethiol moiety at the 5' end. This immobilization strategy optimizes the orientation of the aptamer on the surface and facilitates the folding on the binding target. Under optimized assay conditions, one can determine cocaine at a concentration of 1 muM, which compares favorably with analogous aptameric sensors based on electrochemical and fluorescence techniques. The sensor can be readily regenerated by being washed with a buffer. These results suggest that the SERS-based transducer might create a new dimension for future development of aptameric sensors for sensitive determination in biochemical and biomedical studies.

Authors+Show Affiliations

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18666292

Citation

Chen, Jiwei, et al. "A New Aptameric Biosensor for Cocaine Based On Surface-enhanced Raman Scattering Spectroscopy." Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 14, no. 27, 2008, pp. 8374-82.
Chen J, Jiang J, Gao X, et al. A new aptameric biosensor for cocaine based on surface-enhanced Raman scattering spectroscopy. Chemistry. 2008;14(27):8374-82.
Chen, J., Jiang, J., Gao, X., Liu, G., Shen, G., & Yu, R. (2008). A new aptameric biosensor for cocaine based on surface-enhanced Raman scattering spectroscopy. Chemistry (Weinheim an Der Bergstrasse, Germany), 14(27), 8374-82. https://doi.org/10.1002/chem.200701307
Chen J, et al. A New Aptameric Biosensor for Cocaine Based On Surface-enhanced Raman Scattering Spectroscopy. Chemistry. 2008;14(27):8374-82. PubMed PMID: 18666292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new aptameric biosensor for cocaine based on surface-enhanced Raman scattering spectroscopy. AU - Chen,Jiwei, AU - Jiang,Jianhui, AU - Gao,Xing, AU - Liu,Guokun, AU - Shen,Guoli, AU - Yu,Ruqin, PY - 2008/7/31/pubmed PY - 2008/12/17/medline PY - 2008/7/31/entrez SP - 8374 EP - 82 JF - Chemistry (Weinheim an der Bergstrasse, Germany) JO - Chemistry VL - 14 IS - 27 N2 - The present study reports the proof of principle of a reagentless aptameric sensor based on surface-enhanced Raman scattering (SERS) spectroscopy with "signal-on" architecture using a model target of cocaine. This new aptameric sensor is based on the conformational change of the surface-tethered aptamer on a binding target that draws a certain Raman reporter in close proximity to the SERS substrate, thereby increasing the Raman scattering signal due to the local enhancement effect of SERS. To improve the response performance, the sensor is fabricated from a cocaine-templated mixed self-assembly of a 3'-terminal tetramethylrhodamine (TMR)-labeled DNA aptamer on a silver colloid film by means of an alkanethiol moiety at the 5' end. This immobilization strategy optimizes the orientation of the aptamer on the surface and facilitates the folding on the binding target. Under optimized assay conditions, one can determine cocaine at a concentration of 1 muM, which compares favorably with analogous aptameric sensors based on electrochemical and fluorescence techniques. The sensor can be readily regenerated by being washed with a buffer. These results suggest that the SERS-based transducer might create a new dimension for future development of aptameric sensors for sensitive determination in biochemical and biomedical studies. SN - 0947-6539 UR - https://www.unboundmedicine.com/medline/citation/18666292/A_new_aptameric_biosensor_for_cocaine_based_on_surface_enhanced_Raman_scattering_spectroscopy_ L2 - https://doi.org/10.1002/chem.200701307 DB - PRIME DP - Unbound Medicine ER -