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Comparison of tandem-in-space and tandem-in-time mass spectrometry in gas chromatography determination of pesticides: application to simple and complex food samples.
J Chromatogr A. 2008 Sep 05; 1203(2):229-38.JC

Abstract

Gas chromatography coupled to tandem mass spectrometry (GC-MS/MS) is one of the most powerful techniques in pesticide residue analysis. MS/MS can be conceived in two ways: tandem in space (e.g. triple quadrupole, QqQ) or in time (e.g. ion trap, IT). QqQ and IT are commonly interfaced to GC; however, there has not been any direct comparison between them in pesticide residue analysis so far. In the present work, the performance of GC coupled to these two analyzers (GC-QqQ-MS/MS and GC-IT-MS/MS) was studied and compared for pesticide residue analysis as well as its application in food analysis. The large volume injection (LVI) technique together with programmed-temperature vaporization (PTV) was applied. For this purpose, 19 pesticides, including organochlorine and organophophorus pesticides and pyrethroids, were analyzed in both systems. Mass spectrometric data, performance characteristics (linearity, intra-day and inter-day precision) and the influence of the matrix nature on the analysis of low concentrations were compared. The target compounds were analyzed in solvent and in two representative food matrices such as cucumber (high water content) and egg (high fat content). MS data and intra-day precision were similar in QqQ and IT, whereas inter-day precision was significantly worse in QqQ. Linearity (expressed as determination coefficient, R(2)) in the range 10-150 microg L(-1) was adequate in both systems; however, better R(2) values were obtained with the QqQ analyzer in high and low concentration ranges (1-50 and 1-750 microg L(-1), respectively). The influence of the matrix nature on the analysis of low concentrations of each analyzer was also evaluated. The QqQ and IT performance was similar in cucumber and solvent. However, QqQ provided better sensitivity in egg working in selected reaction monitoring (SRM).

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Authors+Show Affiliations

Garrido Frenich A
Research Group Analytical Chemistry of Contaminants, Department of Analytical Chemistry, University of Almeria, 04120 Almeria, Spain. agarrido@ual.es
Plaza-Bolaños P
No affiliation info available
Martínez Vidal JL
No affiliation info available

MeSH

EggsFood ContaminationGas Chromatography-Mass SpectrometryPesticide ResiduesPesticidesTandem Mass SpectrometryVegetables

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

18667209

Citation

Garrido Frenich, A, et al. "Comparison of Tandem-in-space and Tandem-in-time Mass Spectrometry in Gas Chromatography Determination of Pesticides: Application to Simple and Complex Food Samples." Journal of Chromatography. A, vol. 1203, no. 2, 2008, pp. 229-38.
Garrido Frenich A, Plaza-Bolaños P, Martínez Vidal JL. Comparison of tandem-in-space and tandem-in-time mass spectrometry in gas chromatography determination of pesticides: application to simple and complex food samples. J Chromatogr A. 2008;1203(2):229-38.
Garrido Frenich, A., Plaza-Bolaños, P., & Martínez Vidal, J. L. (2008). Comparison of tandem-in-space and tandem-in-time mass spectrometry in gas chromatography determination of pesticides: application to simple and complex food samples. Journal of Chromatography. A, 1203(2), 229-38. https://doi.org/10.1016/j.chroma.2008.07.041
Garrido Frenich A, Plaza-Bolaños P, Martínez Vidal JL. Comparison of Tandem-in-space and Tandem-in-time Mass Spectrometry in Gas Chromatography Determination of Pesticides: Application to Simple and Complex Food Samples. J Chromatogr A. 2008 Sep 5;1203(2):229-38. PubMed PMID: 18667209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of tandem-in-space and tandem-in-time mass spectrometry in gas chromatography determination of pesticides: application to simple and complex food samples. AU - Garrido Frenich,A, AU - Plaza-Bolaños,P, AU - Martínez Vidal,J L, Y1 - 2008/07/19/ PY - 2008/04/17/received PY - 2008/07/10/revised PY - 2008/07/16/accepted PY - 2008/8/1/pubmed PY - 2008/10/16/medline PY - 2008/8/1/entrez SP - 229 EP - 38 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1203 IS - 2 N2 - Gas chromatography coupled to tandem mass spectrometry (GC-MS/MS) is one of the most powerful techniques in pesticide residue analysis. MS/MS can be conceived in two ways: tandem in space (e.g. triple quadrupole, QqQ) or in time (e.g. ion trap, IT). QqQ and IT are commonly interfaced to GC; however, there has not been any direct comparison between them in pesticide residue analysis so far. In the present work, the performance of GC coupled to these two analyzers (GC-QqQ-MS/MS and GC-IT-MS/MS) was studied and compared for pesticide residue analysis as well as its application in food analysis. The large volume injection (LVI) technique together with programmed-temperature vaporization (PTV) was applied. For this purpose, 19 pesticides, including organochlorine and organophophorus pesticides and pyrethroids, were analyzed in both systems. Mass spectrometric data, performance characteristics (linearity, intra-day and inter-day precision) and the influence of the matrix nature on the analysis of low concentrations were compared. The target compounds were analyzed in solvent and in two representative food matrices such as cucumber (high water content) and egg (high fat content). MS data and intra-day precision were similar in QqQ and IT, whereas inter-day precision was significantly worse in QqQ. Linearity (expressed as determination coefficient, R(2)) in the range 10-150 microg L(-1) was adequate in both systems; however, better R(2) values were obtained with the QqQ analyzer in high and low concentration ranges (1-50 and 1-750 microg L(-1), respectively). The influence of the matrix nature on the analysis of low concentrations of each analyzer was also evaluated. The QqQ and IT performance was similar in cucumber and solvent. However, QqQ provided better sensitivity in egg working in selected reaction monitoring (SRM). SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18667209/Comparison_of_tandem_in_space_and_tandem_in_time_mass_spectrometry_in_gas_chromatography_determination_of_pesticides:_application_to_simple_and_complex_food_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)01219-3 DB - PRIME DP - Unbound Medicine ER -