Tags

Type your tag names separated by a space and hit enter

Mechanism of different spatial distributions of Caenorhabditis elegans and human STIM1 at resting state.
Cell Calcium. 2009 Jan; 45(1):77-88.CC

Abstract

Recent studies have identified STIM1 and Orai1 as essential and conserved components of the Ca2+ release-activated Ca2+ (CRAC) channel. However, the reason STIM1 exhibits different distributions in nematode Caenorhabditis elegans and in human cells before endoplasmic reticulum (ER) calcium store depletion has not been clarified. Compared to the diffuse ER distribution of human STIM1 (H.STIM1), we found that C. elegans STIM1 (C.STIM1) was pre-oligomerized in puncta at the cell periphery before Ca2+ store depletion when expressed in HEK293 cells. Interestingly, these C.STIM1 puncta failed to induce aggregation of C. elegans Orai1 (C.Orai1), and no CRAC current was detected in quiescent cells. However, upon store depletion, C.Orai1 and C.STIM1 functioned as a pair to locally sense the store depletion signal and to activate the CRAC channel. We substituted the N-terminus of H.STIM1 for the N-terminus of C.STIM1 (H_C.STIM1), which resulted in pre-puncta resting localization. In contrast, by replacing the C-terminus of C.STIM1 with that of H.STIM1, we made a chimeric protein (C.STIM1_H) that exhibited two distribution profiles at resting state, a diffuse ER pattern like H.STIM1, and large aggregates. Taken together, our results suggest that (1) despite highly conserved functional domains, C. elegans STIM1 and human STIM1 display different spatial distributions in HEK293 cells before store depletion; (2) the C.STIM1 puncta at peripheral sites are not sufficient for the aggregation and activation of C.Orai1 in the absence of store depletion; (3) the distinct distributions of C.STIM1 and H.STIM1 at resting state are determined by the cytoplasmic region of STIM1.

Authors+Show Affiliations

Joint Laboratory of the Institute of Biophysics & Huazhong University of Science and Technology, College of life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18667236

Citation

Gao, Shangbang, et al. "Mechanism of Different Spatial Distributions of Caenorhabditis Elegans and Human STIM1 at Resting State." Cell Calcium, vol. 45, no. 1, 2009, pp. 77-88.
Gao S, Fan Y, Chen L, et al. Mechanism of different spatial distributions of Caenorhabditis elegans and human STIM1 at resting state. Cell Calcium. 2009;45(1):77-88.
Gao, S., Fan, Y., Chen, L., Lu, J., Xu, T., & Xu, P. (2009). Mechanism of different spatial distributions of Caenorhabditis elegans and human STIM1 at resting state. Cell Calcium, 45(1), 77-88. https://doi.org/10.1016/j.ceca.2008.06.005
Gao S, et al. Mechanism of Different Spatial Distributions of Caenorhabditis Elegans and Human STIM1 at Resting State. Cell Calcium. 2009;45(1):77-88. PubMed PMID: 18667236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of different spatial distributions of Caenorhabditis elegans and human STIM1 at resting state. AU - Gao,Shangbang, AU - Fan,Yong, AU - Chen,Liangyi, AU - Lu,Jingze, AU - Xu,Tao, AU - Xu,Pingyong, Y1 - 2008/07/29/ PY - 2008/01/11/received PY - 2008/06/13/revised PY - 2008/06/16/accepted PY - 2008/8/1/pubmed PY - 2009/3/10/medline PY - 2008/8/1/entrez SP - 77 EP - 88 JF - Cell calcium JO - Cell Calcium VL - 45 IS - 1 N2 - Recent studies have identified STIM1 and Orai1 as essential and conserved components of the Ca2+ release-activated Ca2+ (CRAC) channel. However, the reason STIM1 exhibits different distributions in nematode Caenorhabditis elegans and in human cells before endoplasmic reticulum (ER) calcium store depletion has not been clarified. Compared to the diffuse ER distribution of human STIM1 (H.STIM1), we found that C. elegans STIM1 (C.STIM1) was pre-oligomerized in puncta at the cell periphery before Ca2+ store depletion when expressed in HEK293 cells. Interestingly, these C.STIM1 puncta failed to induce aggregation of C. elegans Orai1 (C.Orai1), and no CRAC current was detected in quiescent cells. However, upon store depletion, C.Orai1 and C.STIM1 functioned as a pair to locally sense the store depletion signal and to activate the CRAC channel. We substituted the N-terminus of H.STIM1 for the N-terminus of C.STIM1 (H_C.STIM1), which resulted in pre-puncta resting localization. In contrast, by replacing the C-terminus of C.STIM1 with that of H.STIM1, we made a chimeric protein (C.STIM1_H) that exhibited two distribution profiles at resting state, a diffuse ER pattern like H.STIM1, and large aggregates. Taken together, our results suggest that (1) despite highly conserved functional domains, C. elegans STIM1 and human STIM1 display different spatial distributions in HEK293 cells before store depletion; (2) the C.STIM1 puncta at peripheral sites are not sufficient for the aggregation and activation of C.Orai1 in the absence of store depletion; (3) the distinct distributions of C.STIM1 and H.STIM1 at resting state are determined by the cytoplasmic region of STIM1. SN - 1532-1991 UR - https://www.unboundmedicine.com/medline/citation/18667236/Mechanism_of_different_spatial_distributions_of_Caenorhabditis_elegans_and_human_STIM1_at_resting_state_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4160(08)00108-5 DB - PRIME DP - Unbound Medicine ER -