Tags

Type your tag names separated by a space and hit enter

Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type beta-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005--the new 'MRSAs'?
J Antimicrob Chemother. 2008 Nov; 62(5):978-85.JA

Abstract

OBJECTIVES

To investigate the molecular epidemiology of ciprofloxacin-resistant CTX-M-15-producing Klebsiella pneumoniae epidemic clones (ECs) isolated from six nosocomial outbreaks and sporadic cases during 2005 in Hungary.

METHODS

Two hundred and eighty-one extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae clinical isolates collected from 41 centres were submitted to the National ESBL Reference Laboratory for further investigations. Of the 281 strains, 75 isolates proved to be SHV producers, whereas 6 isolates were ciprofloxacin-susceptible CTX-M-type ESBL producers. One hundred and ninety-six ciprofloxacin-resistant CTX-M-type beta-lactamase-producing isolates collected from 35 centres were subjected to macrorestriction profile analysis. Furthermore, molecular typing was performed by PCR and sequencing of several antibiotic resistance genes, plasmid profile analysis, transfer of resistance determinants and multilocus sequence typing (MLST).

RESULTS

PFGE revealed the existence of three genetic clusters defined as ECs, where 129 isolates belonged to the previously described Hungarian EC (HEC), 46 isolates to epidemic clone II (EC II) and 21 isolates to epidemic clone III (EC III), respectively. All isolates harboured plasmids ranging from 2.0 to 230 kb. PstI digestion of plasmid DNA from transconjugants/transformants revealed diverse restriction patterns from distinct ECs. Sequence analysis of beta-lactamase genes from 19 selected isolates detected bla(CTX-M-15) and bla(OXA-1) in strains from all three ECs and bla(TEM-1) in EC III isolates located on large plasmids. ISEcpI associated with CTX-M-15 was detected only on a 50 kb non-conjugative plasmid from EC III. MLST identified three allelic profiles: ST 15 (HEC), ST 11 (EC III) and the novel ST 147 (EC II), which correspond to the PFGE clusters, respectively.

CONCLUSIONS

In 2005, 97% of all CTX-M-producing K. pneumoniae isolates detected across Hungary were highly ciprofloxacin-resistant CTX-M-15 producers and represented just three stable genetic clones.

Authors+Show Affiliations

Department of Phage Typing and Molecular Epidemiology, National Center for Epidemiology, Gyáli út 2-6, 1097 Budapest, Hungary. damjanova.ivelina@oek.antsz.huNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18667450

Citation

Damjanova, Ivelina, et al. "Expansion and Countrywide Dissemination of ST11, ST15 and ST147 Ciprofloxacin-resistant CTX-M-15-type Beta-lactamase-producing Klebsiella Pneumoniae Epidemic Clones in Hungary in 2005--the New 'MRSAs'?" The Journal of Antimicrobial Chemotherapy, vol. 62, no. 5, 2008, pp. 978-85.
Damjanova I, Tóth A, Pászti J, et al. Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type beta-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005--the new 'MRSAs'? J Antimicrob Chemother. 2008;62(5):978-85.
Damjanova, I., Tóth, A., Pászti, J., Hajbel-Vékony, G., Jakab, M., Berta, J., Milch, H., & Füzi, M. (2008). Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type beta-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005--the new 'MRSAs'? The Journal of Antimicrobial Chemotherapy, 62(5), 978-85. https://doi.org/10.1093/jac/dkn287
Damjanova I, et al. Expansion and Countrywide Dissemination of ST11, ST15 and ST147 Ciprofloxacin-resistant CTX-M-15-type Beta-lactamase-producing Klebsiella Pneumoniae Epidemic Clones in Hungary in 2005--the New 'MRSAs'. J Antimicrob Chemother. 2008;62(5):978-85. PubMed PMID: 18667450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type beta-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005--the new 'MRSAs'? AU - Damjanova,Ivelina, AU - Tóth,Akos, AU - Pászti,Judit, AU - Hajbel-Vékony,Gabriella, AU - Jakab,Melinda, AU - Berta,Judit, AU - Milch,Hedda, AU - Füzi,Miklós, Y1 - 2008/07/30/ PY - 2008/8/1/pubmed PY - 2008/12/17/medline PY - 2008/8/1/entrez SP - 978 EP - 85 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 62 IS - 5 N2 - OBJECTIVES: To investigate the molecular epidemiology of ciprofloxacin-resistant CTX-M-15-producing Klebsiella pneumoniae epidemic clones (ECs) isolated from six nosocomial outbreaks and sporadic cases during 2005 in Hungary. METHODS: Two hundred and eighty-one extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae clinical isolates collected from 41 centres were submitted to the National ESBL Reference Laboratory for further investigations. Of the 281 strains, 75 isolates proved to be SHV producers, whereas 6 isolates were ciprofloxacin-susceptible CTX-M-type ESBL producers. One hundred and ninety-six ciprofloxacin-resistant CTX-M-type beta-lactamase-producing isolates collected from 35 centres were subjected to macrorestriction profile analysis. Furthermore, molecular typing was performed by PCR and sequencing of several antibiotic resistance genes, plasmid profile analysis, transfer of resistance determinants and multilocus sequence typing (MLST). RESULTS: PFGE revealed the existence of three genetic clusters defined as ECs, where 129 isolates belonged to the previously described Hungarian EC (HEC), 46 isolates to epidemic clone II (EC II) and 21 isolates to epidemic clone III (EC III), respectively. All isolates harboured plasmids ranging from 2.0 to 230 kb. PstI digestion of plasmid DNA from transconjugants/transformants revealed diverse restriction patterns from distinct ECs. Sequence analysis of beta-lactamase genes from 19 selected isolates detected bla(CTX-M-15) and bla(OXA-1) in strains from all three ECs and bla(TEM-1) in EC III isolates located on large plasmids. ISEcpI associated with CTX-M-15 was detected only on a 50 kb non-conjugative plasmid from EC III. MLST identified three allelic profiles: ST 15 (HEC), ST 11 (EC III) and the novel ST 147 (EC II), which correspond to the PFGE clusters, respectively. CONCLUSIONS: In 2005, 97% of all CTX-M-producing K. pneumoniae isolates detected across Hungary were highly ciprofloxacin-resistant CTX-M-15 producers and represented just three stable genetic clones. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/18667450/Expansion_and_countrywide_dissemination_of_ST11_ST15_and_ST147_ciprofloxacin_resistant_CTX_M_15_type_beta_lactamase_producing_Klebsiella_pneumoniae_epidemic_clones_in_Hungary_in_2005__the_new_'MRSAs' L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkn287 DB - PRIME DP - Unbound Medicine ER -