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Functional characterization of ergothioneine transport by rat organic cation/carnitine transporter Octn1 (slc22a4).
Biol Pharm Bull. 2008 Aug; 31(8):1580-4.BP

Abstract

It has been reported that organic cation/carnitine transporter 1 (OCTN1) is associated with rheumatoid arthritis and Crohn's disease. Additionally, we reported that OCTN1 is expressed in hematopoietic cells, and is associated with proliferation and differentiation of erythroid cells. However, physiological role of OCTN1 is still unclear. Ergothioneine, an anti-oxidant, was recently reported to be a good substrate of human OCTN1. However, the transport characteristics of ergothioneine in rat remains to be clarified. The present study, is to further investigate the role of rat Octn1 on transport of ergothioneine in rat Octn1 transfected cells and natively expressing cell line PC12 derived from rat adrenal pheochromocytoma. [(3)H]Ergothioneine uptake by rat Octn1 stably transfected HEK293 cells was saturable, sodium dependent with 1 : 1 stoichiometry of ergothioneine, and pH dependent. Since ergothioneine was reported to presumably play a protective role against oxidative stress-induced apoptosis in PC12 cells, its transport in this cell line was investigated. The expression of rat Octn1 and a saturable and Na(+)-dependent transport of ergothioneine were observed in PC12 cells, suggesting that ergothioneine transport in this cell line may be mediated by rat Octn1. These findings suggested that rat Octn1 may act as a survival factor by taking up ergothioneine to suppress oxidative stress in this cell line. In conclusion, functional characteristics of ergothioneine transport by rat Octn1 is similar to that of human OCTN1 and it is suggested that rat Octn1 is important by transporting anti-oxidant ergothioneine in PC12 cells, though its role in vivo is to be investigated.

Authors+Show Affiliations

Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18670092

Citation

Nakamura, Toshimichi, et al. "Functional Characterization of Ergothioneine Transport By Rat Organic Cation/carnitine Transporter Octn1 (slc22a4)." Biological & Pharmaceutical Bulletin, vol. 31, no. 8, 2008, pp. 1580-4.
Nakamura T, Yoshida K, Yabuuchi H, et al. Functional characterization of ergothioneine transport by rat organic cation/carnitine transporter Octn1 (slc22a4). Biol Pharm Bull. 2008;31(8):1580-4.
Nakamura, T., Yoshida, K., Yabuuchi, H., Maeda, T., & Tamai, I. (2008). Functional characterization of ergothioneine transport by rat organic cation/carnitine transporter Octn1 (slc22a4). Biological & Pharmaceutical Bulletin, 31(8), 1580-4.
Nakamura T, et al. Functional Characterization of Ergothioneine Transport By Rat Organic Cation/carnitine Transporter Octn1 (slc22a4). Biol Pharm Bull. 2008;31(8):1580-4. PubMed PMID: 18670092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional characterization of ergothioneine transport by rat organic cation/carnitine transporter Octn1 (slc22a4). AU - Nakamura,Toshimichi, AU - Yoshida,Kenji, AU - Yabuuchi,Hikaru, AU - Maeda,Tomoji, AU - Tamai,Ikumi, PY - 2008/8/2/pubmed PY - 2008/10/1/medline PY - 2008/8/2/entrez SP - 1580 EP - 4 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 31 IS - 8 N2 - It has been reported that organic cation/carnitine transporter 1 (OCTN1) is associated with rheumatoid arthritis and Crohn's disease. Additionally, we reported that OCTN1 is expressed in hematopoietic cells, and is associated with proliferation and differentiation of erythroid cells. However, physiological role of OCTN1 is still unclear. Ergothioneine, an anti-oxidant, was recently reported to be a good substrate of human OCTN1. However, the transport characteristics of ergothioneine in rat remains to be clarified. The present study, is to further investigate the role of rat Octn1 on transport of ergothioneine in rat Octn1 transfected cells and natively expressing cell line PC12 derived from rat adrenal pheochromocytoma. [(3)H]Ergothioneine uptake by rat Octn1 stably transfected HEK293 cells was saturable, sodium dependent with 1 : 1 stoichiometry of ergothioneine, and pH dependent. Since ergothioneine was reported to presumably play a protective role against oxidative stress-induced apoptosis in PC12 cells, its transport in this cell line was investigated. The expression of rat Octn1 and a saturable and Na(+)-dependent transport of ergothioneine were observed in PC12 cells, suggesting that ergothioneine transport in this cell line may be mediated by rat Octn1. These findings suggested that rat Octn1 may act as a survival factor by taking up ergothioneine to suppress oxidative stress in this cell line. In conclusion, functional characteristics of ergothioneine transport by rat Octn1 is similar to that of human OCTN1 and it is suggested that rat Octn1 is important by transporting anti-oxidant ergothioneine in PC12 cells, though its role in vivo is to be investigated. SN - 0918-6158 UR - https://www.unboundmedicine.com/medline/citation/18670092/Functional_characterization_of_ergothioneine_transport_by_rat_organic_cation/carnitine_transporter_Octn1__slc22a4__ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/bpb/31.1580?from=PubMed DB - PRIME DP - Unbound Medicine ER -