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Genotype and mating type analysis of Cryptococcus neoformans and Cryptococcus gattii isolates from China that mainly originated from non-HIV-infected patients.
FEMS Yeast Res. 2008 Sep; 8(6):930-8.FY

Abstract

Cryptococcosis has been reported to be mostly associated with non-HIV-related patients in China. However, little is known about the molecular characteristics of clinical isolates from the Cryptococcus neoformans species complex in this country. In this study, 115 clinical isolates were included. Molecular type VNI was the most representative (n=103), followed by VGI (n=8), VNIII (n=2), VNIV (n=1), and VGII (n=1). With the exception of a serotype D mating type a isolate, all possessed the MATalpha locus. Multilocus sequence typing (MLST) revealed that most Cryptococcus gattii isolates from China shared identical MLST profiles with the most common MLST genotype reported in the VGI group, and the only one VGII isolate resembled the Vancouver Island outbreak minor genotype. The C. gattii strains involved in this study were successfully grouped according to their molecular type and mating types by PCR-restriction fragment length polymorphism (RFLP) analysis of the GEF1 gene. Our results suggest that (1) in China, cryptococcosis is mostly caused by C. neoformans var. grubii (molecular type VNI), and mating type alpha; (2) The most common causative agents of C. gattii infection in China are closely related to a widely distributed MLST genotype; and (3) The PCR-RFLP analysis of the GEF1 gene has the potential to identify the molecular and mating types of C. gattii simultaneously.

Links

Publisher Full Text
academic.oup.com
onlinelibrary.wiley.com
onlinelibrary.wiley.com

Authors+Show Affiliations

Feng X
Medical Mycology Laboratory, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Yao Z
No affiliation info available
Ren D
No affiliation info available
Liao W
No affiliation info available
Wu J
No affiliation info available

MeSH

ChinaCryptococcosisCryptococcusCryptococcus neoformansFungal ProteinsGenes, Mating Type, FungalGenotypeHumansMolecular EpidemiologyPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSequence Analysis, DNASerotyping

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18671745

Citation

Feng, Xiaobo, et al. "Genotype and Mating Type Analysis of Cryptococcus Neoformans and Cryptococcus Gattii Isolates From China That Mainly Originated From non-HIV-infected Patients." FEMS Yeast Research, vol. 8, no. 6, 2008, pp. 930-8.
Feng X, Yao Z, Ren D, et al. Genotype and mating type analysis of Cryptococcus neoformans and Cryptococcus gattii isolates from China that mainly originated from non-HIV-infected patients. FEMS Yeast Res. 2008;8(6):930-8.
Feng, X., Yao, Z., Ren, D., Liao, W., & Wu, J. (2008). Genotype and mating type analysis of Cryptococcus neoformans and Cryptococcus gattii isolates from China that mainly originated from non-HIV-infected patients. FEMS Yeast Research, 8(6), 930-8. https://doi.org/10.1111/j.1567-1364.2008.00422.x
Feng X, et al. Genotype and Mating Type Analysis of Cryptococcus Neoformans and Cryptococcus Gattii Isolates From China That Mainly Originated From non-HIV-infected Patients. FEMS Yeast Res. 2008;8(6):930-8. PubMed PMID: 18671745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotype and mating type analysis of Cryptococcus neoformans and Cryptococcus gattii isolates from China that mainly originated from non-HIV-infected patients. AU - Feng,Xiaobo, AU - Yao,Zhirong, AU - Ren,Daming, AU - Liao,Wanqing, AU - Wu,Jingsong, Y1 - 2008/07/29/ PY - 2008/8/2/pubmed PY - 2008/12/17/medline PY - 2008/8/2/entrez SP - 930 EP - 8 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 6 N2 - Cryptococcosis has been reported to be mostly associated with non-HIV-related patients in China. However, little is known about the molecular characteristics of clinical isolates from the Cryptococcus neoformans species complex in this country. In this study, 115 clinical isolates were included. Molecular type VNI was the most representative (n=103), followed by VGI (n=8), VNIII (n=2), VNIV (n=1), and VGII (n=1). With the exception of a serotype D mating type a isolate, all possessed the MATalpha locus. Multilocus sequence typing (MLST) revealed that most Cryptococcus gattii isolates from China shared identical MLST profiles with the most common MLST genotype reported in the VGI group, and the only one VGII isolate resembled the Vancouver Island outbreak minor genotype. The C. gattii strains involved in this study were successfully grouped according to their molecular type and mating types by PCR-restriction fragment length polymorphism (RFLP) analysis of the GEF1 gene. Our results suggest that (1) in China, cryptococcosis is mostly caused by C. neoformans var. grubii (molecular type VNI), and mating type alpha; (2) The most common causative agents of C. gattii infection in China are closely related to a widely distributed MLST genotype; and (3) The PCR-RFLP analysis of the GEF1 gene has the potential to identify the molecular and mating types of C. gattii simultaneously. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/18671745/Genotype_and_mating_type_analysis_of_Cryptococcus_neoformans_and_Cryptococcus_gattii_isolates_from_China_that_mainly_originated_from_non_HIV_infected_patients_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/j.1567-1364.2008.00422.x DB - PRIME DP - Unbound Medicine ER -