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HPLC determination of extractable and unextractable proanthocyanidins in plant materials.
J Agric Food Chem. 2008 Sep 10; 56(17):7617-24.JA

Abstract

This study developed a method for the determination of extractable and unextractable proanthocyanidins. Extractable proanthocyanidins were separated according to their degree of polymerization using normal phase HPLC. Unextractable proanthocyanidins were measured after acid-catalyzed depolymerization as flavan-3-ols (terminal units) and benzylthioethers (external units). Electrospray ionization mass spectrometry (ESI-MS) was used for the identification of proanthocyanidins in the samples. Hubaux-Vos detection limits were 0.01-0.15 ng/injection for extractable proanthocyanidins, with recovery rates from 69 to 91%. Detection limits for unextractable proanthocyanidin derivatives were 0.002-0.035 ng/injection with 80% recovery. The developed method was applied to the analysis of several fruit and berry samples. Results showed great variation in the proportion of unextractable proanthocyanidins in total proanthocyanidin content between samples, being highest in the green variety of table grape (63%) and lowest in the apple cultivar 'Valkeakuulas' (4.1%). The method reported herein is reliable and gives valuable information on the nature of proanthocyanidins in plant-derived foods.

Authors+Show Affiliations

Food Research, MTT Agrifood Research Finland, Myllytie 1, FI 31600, Jokioinen, Finland. jarkko.hellstrom@mtt.fiNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18672884

Citation

Hellström, Jarkko K., and Pirjo H. Mattila. "HPLC Determination of Extractable and Unextractable Proanthocyanidins in Plant Materials." Journal of Agricultural and Food Chemistry, vol. 56, no. 17, 2008, pp. 7617-24.
Hellström JK, Mattila PH. HPLC determination of extractable and unextractable proanthocyanidins in plant materials. J Agric Food Chem. 2008;56(17):7617-24.
Hellström, J. K., & Mattila, P. H. (2008). HPLC determination of extractable and unextractable proanthocyanidins in plant materials. Journal of Agricultural and Food Chemistry, 56(17), 7617-24. https://doi.org/10.1021/jf801336s
Hellström JK, Mattila PH. HPLC Determination of Extractable and Unextractable Proanthocyanidins in Plant Materials. J Agric Food Chem. 2008 Sep 10;56(17):7617-24. PubMed PMID: 18672884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC determination of extractable and unextractable proanthocyanidins in plant materials. AU - Hellström,Jarkko K, AU - Mattila,Pirjo H, Y1 - 2008/08/02/ PY - 2008/8/5/pubmed PY - 2008/11/1/medline PY - 2008/8/5/entrez SP - 7617 EP - 24 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 56 IS - 17 N2 - This study developed a method for the determination of extractable and unextractable proanthocyanidins. Extractable proanthocyanidins were separated according to their degree of polymerization using normal phase HPLC. Unextractable proanthocyanidins were measured after acid-catalyzed depolymerization as flavan-3-ols (terminal units) and benzylthioethers (external units). Electrospray ionization mass spectrometry (ESI-MS) was used for the identification of proanthocyanidins in the samples. Hubaux-Vos detection limits were 0.01-0.15 ng/injection for extractable proanthocyanidins, with recovery rates from 69 to 91%. Detection limits for unextractable proanthocyanidin derivatives were 0.002-0.035 ng/injection with 80% recovery. The developed method was applied to the analysis of several fruit and berry samples. Results showed great variation in the proportion of unextractable proanthocyanidins in total proanthocyanidin content between samples, being highest in the green variety of table grape (63%) and lowest in the apple cultivar 'Valkeakuulas' (4.1%). The method reported herein is reliable and gives valuable information on the nature of proanthocyanidins in plant-derived foods. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/18672884/HPLC_determination_of_extractable_and_unextractable_proanthocyanidins_in_plant_materials_ L2 - https://dx.doi.org/10.1021/jf801336s DB - PRIME DP - Unbound Medicine ER -